Restriction map for
pFN21AE1950
(Circular) (Six-base) MAPSORT of: pFN21AE1950.seq Check: 3821 from: 1 to: 6226
pFN21AE1950 6226 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4036 278
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3569 1937
Size: 1632 4594
Acc65I G'GTAC_C
Cuts at: 3547 3547
Size: 6226
AclI AA'CG_TT
Cuts at: 1563 3417 4353 4726 1563
Size: 1854 936 373 3063
Fragments arranged by size:
3063 1854 936 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 2883 3465 4289 5673 5941 1632
Size: 1067 184 582 824 1384 268 1917
Fragments arranged by size:
1917 1384 1067 824 582 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 3382 5125 6102 1203
Size: 2179 1743 977 1327
Fragments arranged by size:
2179 1743 1327 977
AgeI A'CCGG_T
Cuts at: 5057 5057
Size: 6226
AhdI GACnn_n'nnGTC
Cuts at: 2492 4955 2492
Size: 2463 3763
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2175 1184
Size: 991 5235
AlwNI CAG_nnn'CTG
Cuts at: 2946 3969 5541 2946
Size: 1023 1572 3631
Fragments arranged by size:
3631 1572 1023
ApaLI G'TGCA_C
Cuts at: 4283 5439 4283
Size: 1156 5070
ApoI r'AATT_y
Cuts at: 1493 2458 3535 3761 3913 6022 1493
Size: 965 1077 226 152 2109 1697
Fragments arranged by size:
2109 1697 1077 965 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2409 2478 2603 4780 160
Size: 872 1377 69 125 2177 1606
Fragments arranged by size:
2177 1606 1377 872 125 69
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 3551 1870
Size: 73 1608 4545
Fragments arranged by size:
4545 1608 73
BamHI G'GATC_C
Cuts at: 3245 3556 3923 3245
Size: 311 367 5548
Fragments arranged by size:
5548 367 311
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3547 5002 618
Size: 325 217 627 1760 1455 1842
Fragments arranged by size:
1842 1760 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3545 1391
Size: 2154 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 3419 928
Size: 971 465 1055 3735
Fragments arranged by size:
3735 1055 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4417 4451 1903
Size: 34 2480 34 3678
Fragments arranged by size:
3678 2480 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3394 4118 5328 3394
Size: 724 1210 4292
Fragments arranged by size:
4292 1210 724
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4837 136
Size: 107 122 71 631 605 3165 1525
Fragments arranged by size:
3165 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 3643 3643
Size: 6226
Bme1580I G_kGCm'C
Cuts at: 1790 4287 5443 1790
Size: 2497 1156 2573
Fragments arranged by size:
2573 2497 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3003 3957 4915
Size: 416 53 83 399 24 1586 954 958
Cuts at: 4915 442
Size: 1753
Fragments arranged by size:
1753 1586 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4886 1239
Size: 3647 2579
Bpu10I CC'TnA_GC
Cuts at: 2939 2939
Size: 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2655 3324 3447 4351 5216 5514 2655
Size: 669 123 904 865 298 3367
Fragments arranged by size:
3367 904 865 669 298 123
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 4889 882
Size: 617 703 2687 2219
Fragments arranged by size:
2687 2219 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 3385 3455 4017 493
Size: 711 2181 70 562 2702
Fragments arranged by size:
2702 2181 711 562 70
BsaBI GATnn'nnATC
Cuts at: 3057 6220 3057
Size: 3163 3063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4033 4415 5050 275
Size: 53 83 186 936 2500 382 635 1451
Fragments arranged by size:
2500 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4659 5057 5331 5478 5889 4659
Size: 398 274 147 411 4996
Fragments arranged by size:
4996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2118 2148 1178
Size: 30 165 30 715 30 5256
Fragments arranged by size:
5256 715 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6226
BseYI C'CCAG_C
Cuts at: 5429 5925 5429
Size: 496 5730
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3289 1163
Size: 573 1553 4100
Fragments arranged by size:
4100 1553 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3676 4437 5465 1540
Size: 275 186 1675 761 1028 2301
Fragments arranged by size:
2301 1675 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4287 4372 5443 4287
Size: 85 1071 5070
Fragments arranged by size:
5070 1071 85
BsmI GAATG_Cn'
Cuts at: 3381 3737 3830 3381
Size: 356 93 5777
Fragments arranged by size:
5777 356 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3545 4287 4372 5443 1391
Size: 399 1755 742 85 1071 2174
Fragments arranged by size:
2174 1755 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5889 5889
Size: 6226
BspHI T'CATG_A
Cuts at: 2053 4115 5845 2053
Size: 2062 1730 2434
Fragments arranged by size:
2434 2062 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3581 844
Size: 2737 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 1394
Size: 429 2290 3507
Fragments arranged by size:
3507 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2716 2850 3090 3521 4721 4895 65
Size: 1183 1468 134 240 431 1200 174 1396
Fragments arranged by size:
1468 1396 1200 1183 431 240 174 134
BsrFI r'CCGG_y
Cuts at: 1700 1810 4870 5057 1700
Size: 110 3060 187 2869
Fragments arranged by size:
3060 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2071 96
Size: 1975 4251
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6226
BssSI C'ACGA_G
Cuts at: 4286 5298 4286
Size: 1012 5214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6226
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6226
BstXI CCAn_nnnn'nTGG
Cuts at: 3047 3047
Size: 6226
BstYI r'GATC_y
Cuts at: 1972 3245 3556 3923 4308 4325 5777 5863 5875
Size: 1273 311 367 385 17 1452 86 12
Cuts at: 5875 6221 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1273 385 367 346 311 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2212 2378 513
Size: 552 1147 166 4361
Fragments arranged by size:
4361 1147 552 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5487
Fragments arranged by size:
5487 683 56
BtsI GCAGTG_nn'
Cuts at: 3742 4536 4556 3742
Size: 794 20 5412
Fragments arranged by size:
5412 794 20
ClaI AT'CG_AT
Cuts at: 1554 2502 1554
Size: 948 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6191
DraI TTT'AAA
Cuts at: 2422 3530 2422
Size: 1108 5118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5233 809
Size: 507 3917 1802
Fragments arranged by size:
3917 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3673 4562 6191
Size: 54 1425 50 275 18 1843 889 1629
Cuts at: 6191 8
Size: 43
Fragments arranged by size:
1843 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3673 1537
Size: 275 1861 4090
Fragments arranged by size:
4090 1861 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2859 3248 4156 1353
Size: 1506 389 908 3423
Fragments arranged by size:
3423 1506 908 389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4813 5187 5333 387
Size: 1064 138 3224 374 146 1280
Fragments arranged by size:
3224 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2699 2883 3465 4289 4886 5673 5941
Size: 393 1067 184 582 824 597 787 268
Cuts at: 5941 1239
Size: 1524
Fragments arranged by size:
1524 1067 824 787 597 582 393 268
184
EcoNI CCTnn'n_nnAGG
Cuts at: 3315 3315
Size: 6226
EcoRI G'AATT_C
Cuts at: 2458 3535 3913 2458
Size: 1077 378 4771
Fragments arranged by size:
4771 1077 378
EcoRV GAT'ATC
Cuts at: 3172 3172
Size: 6226
FspI TGC'GCA
Cuts at: 4732 4732
Size: 6226
HaeII r_GCGC'y
Cuts at: 1678 5373 1678
Size: 3695 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2478 2510 2579 2606 2611
Size: 32 135 32 906 32 69 27 5
Cuts at: 2611 2633 2638 2665 4867 4899 4941 4973 1373
Size: 22 5 27 2202 32 42 32 2626
Fragments arranged by size:
2626 2202 906 135 69 42 32 32
32 32 32 27 27 22 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3570 3816 669
Size: 988 281 1632 246 3079
Fragments arranged by size:
3079 1632 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3465 748
Size: 2717 3509
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KpnI G_GTAC'C
Cuts at: 3551 3551
Size: 6226
MfeI C'AATT_G
Cuts at: 3825 3825
Size: 6226
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5340 5524 1305
Size: 4035 184 2007
Fragments arranged by size:
4035 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4404
Fragments arranged by size:
4404 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2832 4184 4543 4702 518
Size: 648 1071 595 1352 359 159 2042
Fragments arranged by size:
2042 1352 1071 648 595 359 159
MspA1I CmG'CkG
Cuts at: 1606 2159 3642 4319 5467 5712 1606
Size: 553 1483 677 1148 245 2120
Fragments arranged by size:
2120 1483 1148 677 553 245
NcoI C'CATG_G
Cuts at: 513 1065 2378 513
Size: 552 1313 4361
Fragments arranged by size:
4361 1313 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6226
NotI GC'GGCC_GC
Cuts at: 3673 3673
Size: 6226
NspI r_CATG'y
Cuts at: 1132 1229 2697 3584 5129 1132
Size: 97 1468 887 1545 2229
Fragments arranged by size:
2229 1545 1468 887 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6226
PciI A'CATG_T
Cuts at: 5125 5125
Size: 6226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2987 3024 1108
Size: 1879 37 4310
Fragments arranged by size:
4310 1879 37
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6226
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PstI C_TGCA'G
Cuts at: 830 2164 3439 3578 830
Size: 1334 1275 139 3478
Fragments arranged by size:
3478 1334 1275 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
PvuII CAG'CTG
Cuts at: 2159 2159
Size: 6226
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SbfI CC_TGCA'GG
Cuts at: 3578 3578
Size: 6226
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SfcI C'TryA_G
Cuts at: 826 1045 2160 3435 3574 4709 5390 5581 826
Size: 219 1115 1275 139 1135 681 191 1471
Fragments arranged by size:
1471 1275 1135 1115 681 219 191 139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6226
SmaI CCC'GGG
Cuts at: 1872 3553 1872
Size: 1681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2670 3339 3462 4330 5231 5493
Size: 197 926 727 669 123 868 901 262
Cuts at: 5493 820
Size: 1553
Fragments arranged by size:
1553 926 901 868 727 669 262 197
123
SnaBI TAC'GTA
Cuts at: 493 3455 4017 493
Size: 2962 562 2702
Fragments arranged by size:
2962 2702 562
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 3584 1132
Size: 2452 3774
SspI AAT'ATT
Cuts at: 5 52 4150 5
Size: 47 4098 2081
Fragments arranged by size:
4098 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6226
StyI C'CwwG_G
Cuts at: 513 1065 2378 3360 3665 513
Size: 552 1313 982 305 3074
Fragments arranged by size:
3074 1313 982 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3400 4253 4270 4423 4608 5053 325
Size: 1255 1820 853 17 153 185 445 1498
Fragments arranged by size:
1820 1498 1255 853 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2071 3328 4472
Size: 274 80 33 51 1443 94 1257 1144
Cuts at: 4472 6184 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1257 1144 274 138 94 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6186 6218 6186
Size: 32 6194
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6226
XbaI T'CTAG_A
Cuts at: 3562 3562
Size: 6226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 6226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6226
XmaI C'CCGG_G
Cuts at: 1870 3551 1870
Size: 1681 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 4355 2340
Size: 2015 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4034 276
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI