################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvrII BamHI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaBI BsaI BsaWI BseAI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI MaeIII MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 622 GTCTAC GT^[AC][GT]AC AfaI 348 GTAC GT^AC Alw44I 514 GTGCAC G^TGCAC Aor51HI 1025 AGCGCT AGC^GCT ApaI 319 GGGCCC GGGCC^C ApaLI 514 GTGCAC G^TGCAC AspI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 191 TGCGCA TGC^GCA BanI 726 GGCGCC G^G[CT][GA]CC BanII 319 GGGCCC G[GA]GC[CT]^C BbeI 726 GGCGCC GGCGC^C BbrPI 477 CACGTG CAC^GTG BcnI 61 CCGGG CC^[GC]GG BfaI 616 CTAG C^TAG BglII 677 AGATCT A^GATCT BlpI 159 GCTGAGC GC^T[ACGT]AGC Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC BsaAI 477 CACGTG [CT]AC^GT[GA] BseRI 1140 GAGGAG GAGGAG(10/8) BsmBI 1075 GAGACG CGTCTC(1/5) BsmI 168 GAATGC GAATGC(1/-1) BspHI 230 TCATGA T^CATGA BsrBI 162 GAGCGG CCGCTC(-3/-3) BsrFI 633 GCCGGT [GA]^CCGG[CT] BsrI 23 ACTGG ACTGG(1/-1) BssSI 1018 CTCGTG CACGAG(-5/-1) BstAPI 684 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 159 GCTGAGC GC^T[ACGT]AGC Cfr10I 633 GCCGGT [GA]^CCGG[CT] EagI 719 CGGCCG C^GGCCG EclXI 719 CGGCCG C^GGCCG Eco47III 1025 AGCGCT AGC^GCT Eco52I 719 CGGCCG C^GGCCG FspI 191 TGCGCA TGC^GCA HphI 302 TCACC GGTGA(8/7) KasI 726 GGCGCC G^GCGCC KspI 94 CCGCGG CCGC^GG MaeI 616 CTAG C^TAG MaeII 478 ACGT A^CGT NarI 726 GGCGCC GG^CGCC NciI 61 CCGGG CC^[GC]GG NheI 615 GCTAGC G^CTAGC PflFI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 477 CACGTG CAC^GTG PmlI 477 CACGTG CAC^GTG PpuMI 585 GGGACCC [GA]G^G[AT]CC[CT] PstI 707 CTGCAG CTGCA^G PvuII 963 CAGCTG CAG^CTG RcaI 230 TCATGA T^CATGA RsaI 348 GTAC GT^AC SacII 94 CCGCGG CCGC^GG ScaI 347 AGTACT AGT^ACT SexAI 254 ACCTGGT A^CC[AT]GGT SfcI 707 CTGCAG C^T[GA][CT]AG SfoI 726 GGCGCC GGC^GCC SmlI 697 CTCAAG C^T[CT][GA]AG StuI 593 AGGCCT AGG^CCT TaqI 554 TCGA T^CGA Tth111I 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 554 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BbsI 550 GTCTTC GAAGAC(2/6) 568 GTCTTC BpmI 1131 CTCCAG CTGGAG(16/14) 24 CTGGAG BpuAI 550 GTCTTC GAAGAC(2/6) 568 GTCTTC BsgI 880 GTGCAG GTGCAG(16/14) 1021 GTGCAG BsiEI 456 CGACCG CG[GA][CT]^CG 719 CGGCCG BsmAI 1075 GAGAC GTCTC(1/5) 140 GTCTC DraII 107 AGGCCCT [GA]G^G[ACGT]CC[CT] 585 GGGACCC DsaI 94 CCGCGG C^C[GA][CT]GG 1160 CCACGG EarI 220 CTCTTC CTCTTC(1/4) 1006 CTCTTC EcoO109I 107 AGGCCCT [GA]G^G[ACGT]CC[CT] 585 GGGACCC FauI 93 CCCGC CCCGC(4/6) 164 GCGGG Ksp632I 220 CTCTTC CTCTTC(1/4) 1006 CTCTTC MslI 834 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 936 CATCGTCATG NspI 365 GCATGT [GA]CATG^[CT] 986 ACATGC SfaNI 901 GATGC GCATC(5/9) 245 GCATC TspRI 266 CAGTG CA[GC]TG[ACGT][ACGT]^ 467 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AspHI AvaII BalI BbvI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI XhoII