(Circular) (Six-base) MAPSORT of: pFN21AE0658.seq Check: 8872 from: 1 to: 5872 pFN21AE0658 5872 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2350 2506 2350 Size: 156 5716 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2628 3215 1937 Size: 691 587 4594 Fragments arranged by size: 4594 691 587 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3080 3935 5319 5587 1632 Size: 1448 855 1384 268 1917 Fragments arranged by size: 1917 1448 1384 855 268 AfeI AGC'GCT Cuts at: 3032 3032 Size: 5872 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AlwNI CAG_nnn'CTG Cuts at: 2284 2296 2437 2973 3615 5187 2284 Size: 12 141 536 642 1572 2969 Fragments arranged by size: 2969 1572 642 536 141 12 ApaI G_GGCC'C Cuts at: 2328 2328 Size: 5872 ApaLI G'TGCA_C Cuts at: 2519 3929 5085 2519 Size: 1410 1156 3306 Fragments arranged by size: 3306 1410 1156 ApoI r'AATT_y Cuts at: 1493 3181 3407 3559 5668 1493 Size: 1688 226 152 2109 1697 Fragments arranged by size: 2109 1697 1688 226 152 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 3197 1870 Size: 73 1254 4545 Fragments arranged by size: 4545 1254 73 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2731 3193 4648 618 Size: 325 217 627 944 462 1455 1842 Fragments arranged by size: 1842 1455 944 627 462 325 217 BanII G_rGCy'C Cuts at: 1391 2328 3191 1391 Size: 937 863 4072 Fragments arranged by size: 4072 937 863 BbeI G_GCGC'C Cuts at: 2735 2735 Size: 5872 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2548 2566 928 Size: 971 649 18 4234 Fragments arranged by size: 4234 971 649 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2682 5867 2682 Size: 3185 2687 BlpI GC'TnA_GC Cuts at: 2165 3289 2165 Size: 1124 4748 Bme1580I G_kGCm'C Cuts at: 1790 2328 2523 3933 5089 1790 Size: 538 195 1410 1156 2573 Fragments arranged by size: 2573 1410 1156 538 195 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3603 4561 442 Size: 416 53 83 399 24 2186 958 1753 Fragments arranged by size: 2186 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2624 1056 Size: 1568 4304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2050 3121 4532 1239 Size: 811 1071 1411 2579 Fragments arranged by size: 2579 1411 1071 811 Bpu10I CC'TnA_GC Cuts at: 2246 3122 2246 Size: 876 4996 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2687 3997 4862 5160 2687 Size: 1310 865 298 3399 Fragments arranged by size: 3399 1310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2484 3663 493 Size: 711 1280 1179 2702 Fragments arranged by size: 2702 1280 1179 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2553 2732 2870 3070 Size: 53 83 186 936 1020 179 138 200 Cuts at: 3070 3679 4061 4696 275 Size: 609 382 635 1451 Fragments arranged by size: 1451 1020 936 635 609 382 200 186 179 138 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3095 3125 1178 Size: 30 165 30 1692 30 3925 Fragments arranged by size: 3925 1692 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3160 1186 Size: 1974 3898 BseYI C'CCAG_C Cuts at: 2565 3156 5075 5571 2565 Size: 591 1919 496 2866 Fragments arranged by size: 2866 1919 591 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2906 3047 1163 Size: 573 1170 141 3988 Fragments arranged by size: 3988 1170 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2464 2727 3322 4083 5111 1540 Size: 275 186 463 263 595 761 1028 2301 Fragments arranged by size: 2301 1028 761 595 463 275 263 186 BsiHKAI G_wGCw'C Cuts at: 2478 2523 2652 3933 4018 5089 2478 Size: 45 129 1281 85 1071 3261 Fragments arranged by size: 3261 1281 1071 129 85 45 BsmI GAATG_Cn' Cuts at: 2179 3383 3476 2179 Size: 1204 93 4575 Fragments arranged by size: 4575 1204 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3074 3074 Size: 5872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2478 2523 2652 3191 3933 4018 Size: 399 538 150 45 129 539 742 85 Cuts at: 4018 5089 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 539 538 399 150 129 85 45 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 2235 3761 5491 2235 Size: 1526 1730 2616 Fragments arranged by size: 2616 1730 1526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 2350 2397 2506 3227 844 Size: 1496 10 47 109 721 3489 Fragments arranged by size: 3489 1496 721 109 47 10 BsrBI CCG'CTC Cuts at: 1394 1823 2169 3759 1394 Size: 429 346 1590 3507 Fragments arranged by size: 3507 1590 429 346 BsrDI GCAATG_nn' Cuts at: 65 1248 4367 4541 65 Size: 1183 3119 174 1396 Fragments arranged by size: 3119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2638 4516 4703 1700 Size: 110 828 1878 187 2869 Fragments arranged by size: 2869 1878 828 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 2013 2120 2508 1925 Size: 88 107 388 5289 Fragments arranged by size: 5289 388 107 88 BssSI C'ACGA_G Cuts at: 3023 3932 4944 3023 Size: 909 1012 3951 Fragments arranged by size: 3951 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 2695 794 Size: 1901 3971 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstYI r'GATC_y Cuts at: 1972 2682 2814 2985 3202 3569 3954 3971 5423 Size: 710 132 171 217 367 385 17 1452 Cuts at: 5423 5509 5521 5867 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 710 385 367 346 217 171 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2099 3165 513 Size: 552 939 95 1066 3220 Fragments arranged by size: 3220 1066 939 552 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2236 2769 3113 487 Size: 683 56 1010 533 344 3246 Fragments arranged by size: 3246 1010 683 533 344 56 BtsI GCAGTG_nn' Cuts at: 2277 2478 3388 4182 4202 2277 Size: 201 910 794 20 3947 Fragments arranged by size: 3947 910 794 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2421 2502 2724 Size: 54 1425 50 275 18 591 81 222 Cuts at: 2724 2955 3319 4208 5837 8 Size: 231 364 889 1629 43 Fragments arranged by size: 1629 1425 889 591 364 275 231 222 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2724 3319 1537 Size: 275 912 595 4090 Fragments arranged by size: 4090 912 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 3017 3802 1353 Size: 878 786 785 3423 Fragments arranged by size: 3423 878 786 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3037 4459 4833 4979 387 Size: 1064 138 1448 1422 374 146 1280 Fragments arranged by size: 1448 1422 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 3080 3121 3935 4532 5319 5587 Size: 393 418 1030 41 814 597 787 268 Cuts at: 5587 1239 Size: 1524 Fragments arranged by size: 1524 1030 814 787 597 418 393 268 41 EcoO109I rG'GnC_Cy Cuts at: 2113 2591 2113 Size: 478 5394 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FspI TGC'GCA Cuts at: 2198 4378 2198 Size: 2180 3692 HaeII r_GCGC'y Cuts at: 1678 2037 2058 2165 2195 2735 3034 5019 1678 Size: 359 21 107 30 540 299 1985 2531 Fragments arranged by size: 2531 1985 540 359 299 107 30 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373 Size: 32 135 32 2941 32 42 32 2626 Fragments arranged by size: 2941 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KasI G'GCGC_C Cuts at: 2731 2731 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4986 5170 1305 Size: 3681 184 2007 Fragments arranged by size: 3681 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2423 2504 2957 10 Size: 54 1425 343 591 81 453 2925 Fragments arranged by size: 2925 1425 591 453 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 2945 3830 4189 4348 518 Size: 648 1677 102 885 359 159 2042 Fragments arranged by size: 2042 1677 885 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2101 2415 2437 2711 2717 2970 3288 3965 Size: 495 314 22 274 6 253 318 677 Cuts at: 3965 5113 5358 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 495 318 314 274 253 245 22 6 NarI GG'CG_CC Cuts at: 2732 2732 Size: 5872 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4381 Fragments arranged by size: 4381 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 2620 1052 Size: 1568 4304 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 2374 2995 3230 4775 1132 Size: 97 1145 621 235 1545 2229 Fragments arranged by size: 2229 1545 1145 621 235 97 PasI CC'CwG_GG Cuts at: 1348 2586 1348 Size: 1238 4634 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5872 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PmlI CAC'GTG Cuts at: 2484 2484 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2591 2591 Size: 5872 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PspOMI G'GGCC_C Cuts at: 2324 2324 Size: 5872 PstI C_TGCA'G Cuts at: 830 2716 3224 830 Size: 1886 508 3478 Fragments arranged by size: 3478 1886 508 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2970 2970 Size: 5872 SacII CC_GC'GG Cuts at: 2102 2102 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SanDI GG'GwC_CC Cuts at: 2591 2591 Size: 5872 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 2354 4120 5832 2354 Size: 1766 1712 2394 Fragments arranged by size: 2394 1766 1712 SexAI A'CCwGG_T Cuts at: 2259 2259 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 2712 3220 4355 5036 5227 826 Size: 219 1667 508 1135 681 191 1471 Fragments arranged by size: 1667 1471 1135 681 508 219 191 SfoI GGC'GCC Cuts at: 2733 2733 Size: 5872 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 3976 4877 5139 820 Size: 197 926 759 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 759 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 2600 1517 Size: 1083 4789 StyI C'CwwG_G Cuts at: 513 1065 2004 2595 2805 2919 3311 513 Size: 552 939 591 210 114 392 3074 Fragments arranged by size: 3074 939 591 552 392 210 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3899 3916 4069 4254 4699 325 Size: 1255 2319 17 153 185 445 1498 Fragments arranged by size: 2319 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 4118 5830 Size: 274 80 33 51 1443 375 1766 1712 Cuts at: 5830 96 Size: 138 Fragments arranged by size: 1766 1712 1443 375 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2164 2196 5832 5864 2164 Size: 32 3636 32 2172 Fragments arranged by size: 3636 2172 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2552 1316 Size: 1236 4636 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI XcmI