(Circular) (Six-base) MAPSORT of: pFN21AB6651.seq Check: 8817 from: 1 to: 5965 pFN21AB6651 5965 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 1937 Size: 1371 4594 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 4028 5412 5680 1632 Size: 930 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 930 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 4864 5841 1203 Size: 3661 977 1327 Fragments arranged by size: 3661 1327 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3073 1184 Size: 1889 4076 AlwNI CAG_nnn'CTG Cuts at: 2340 2792 3708 5280 2340 Size: 452 916 1572 3025 Fragments arranged by size: 3025 1572 916 452 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 2395 3274 3500 3652 5761 1493 Size: 902 879 226 152 2109 1697 Fragments arranged by size: 2109 1697 902 879 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 3290 1870 Size: 73 1347 4545 Fragments arranged by size: 4545 1347 73 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3286 4741 618 Size: 325 217 627 1499 1455 1842 Fragments arranged by size: 1842 1499 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2945 2967 3284 1391 Size: 1554 22 317 4072 Fragments arranged by size: 4072 1554 317 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2430 928 Size: 971 531 4463 Fragments arranged by size: 4463 971 531 BbvCI CC'TCA_GC Cuts at: 2615 2615 Size: 5965 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 2007 2167 2686 2713 1548 Size: 459 160 519 27 4800 Fragments arranged by size: 4800 519 459 160 27 BfrBI ATG'CAT Cuts at: 2882 2882 Size: 5965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4576 136 Size: 107 122 71 631 605 2904 1525 Fragments arranged by size: 2904 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3154 5960 3154 Size: 2806 3159 BlpI GC'TnA_GC Cuts at: 3382 3382 Size: 5965 Bme1580I G_kGCm'C Cuts at: 1790 4026 5182 1790 Size: 2236 1156 2573 Fragments arranged by size: 2573 2236 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2582 3696 4654 Size: 416 53 83 399 24 1165 1114 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1165 1114 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2641 2989 3255 3269 4625 1239 Size: 1402 348 266 14 1356 2579 Fragments arranged by size: 2579 1402 1356 348 266 14 Bpu10I CC'TnA_GC Cuts at: 2615 2615 Size: 5965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2121 3040 4090 4955 5253 2121 Size: 919 1050 865 298 2833 Fragments arranged by size: 2833 1050 919 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2379 3236 4628 882 Size: 617 880 857 1392 2219 Fragments arranged by size: 2219 1392 880 857 617 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3772 4154 4789 275 Size: 53 83 186 936 2239 382 635 1451 Fragments arranged by size: 2239 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4398 4796 5070 5217 5628 4398 Size: 398 274 147 411 4735 Fragments arranged by size: 4735 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5740 Fragments arranged by size: 5740 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5965 BseYI C'CCAG_C Cuts at: 2340 3185 5168 5664 2340 Size: 845 1983 496 2641 Fragments arranged by size: 2641 1983 845 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2452 2971 1163 Size: 573 716 519 4157 Fragments arranged by size: 4157 716 573 519 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3415 4176 5204 1540 Size: 275 186 1414 761 1028 2301 Fragments arranged by size: 2301 1414 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2945 4026 4111 5182 2945 Size: 1081 85 1071 3728 Fragments arranged by size: 3728 1081 1071 85 BsmI GAATG_Cn' Cuts at: 2552 2882 3476 3569 2552 Size: 330 594 93 4948 Fragments arranged by size: 4948 594 330 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2768 3191 2768 Size: 423 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2945 2967 3284 4026 4111 5182 1391 Size: 399 1155 22 317 742 85 1071 2174 Fragments arranged by size: 2174 1155 1071 742 399 317 85 22 BspEI T'CCGG_A Cuts at: 5628 5628 Size: 5965 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3320 844 Size: 2476 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3852 1394 Size: 429 2029 3507 Fragments arranged by size: 3507 2029 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2806 4460 4634 65 Size: 1183 1558 1654 174 1396 Fragments arranged by size: 1654 1558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4609 4796 1700 Size: 110 2799 187 2869 Fragments arranged by size: 2869 2799 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5965 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5965 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstYI r'GATC_y Cuts at: 1972 3154 3295 3662 4047 4064 5516 5602 5614 Size: 1182 141 367 385 17 1452 86 12 Cuts at: 5614 5960 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2496 2496 Size: 5965 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5413 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 2464 2943 3481 4275 4295 2464 Size: 479 538 794 20 4134 Fragments arranged by size: 4134 794 538 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 3269 3269 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4972 809 Size: 507 3656 1802 Fragments arranged by size: 3656 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 3412 4301 Size: 54 1425 50 275 18 501 1081 889 Cuts at: 4301 5930 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1081 889 501 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3412 1537 Size: 275 1600 4090 Fragments arranged by size: 4090 1600 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3895 1353 Size: 2542 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4552 4926 5072 387 Size: 1064 138 2963 374 146 1280 Fragments arranged by size: 2963 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2943 2943 Size: 5965 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 2641 2989 3255 3269 4028 4625 Size: 393 930 79 348 266 14 759 597 Cuts at: 4625 5412 5680 1239 Size: 787 268 1524 Fragments arranged by size: 1524 930 787 759 597 393 348 268 266 79 14 EcoO109I rG'GnC_Cy Cuts at: 2679 2679 Size: 5965 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2477 2477 Size: 5965 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 5112 1678 Size: 3434 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4606 4638 4680 4712 1373 Size: 32 135 32 3034 32 42 32 2626 Fragments arranged by size: 3034 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2650 3309 3555 669 Size: 988 281 712 659 246 3079 Fragments arranged by size: 3079 988 712 659 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5965 HpaI GTT'AAC Cuts at: 2650 3555 2650 Size: 905 5060 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 2388 3564 2388 Size: 1176 4789 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5079 5263 1305 Size: 3774 184 2007 Fragments arranged by size: 3774 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 10 Size: 54 1425 343 501 3642 Fragments arranged by size: 3642 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3923 4282 4441 518 Size: 648 2757 359 159 2042 Fragments arranged by size: 2757 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2340 2692 3381 4058 5206 5451 1606 Size: 734 352 689 677 1148 245 2120 Fragments arranged by size: 2120 1148 734 689 677 352 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5413 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NsiI A_TGCA'T Cuts at: 2884 2884 Size: 5965 NspI r_CATG'y Cuts at: 1132 1229 2024 2592 3323 4868 1132 Size: 97 795 568 731 1545 2229 Fragments arranged by size: 2229 1545 795 731 568 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5965 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 2340 1108 Size: 1232 4733 PfoI T'CCnGG_A Cuts at: 2377 2377 Size: 5965 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2306 2338 4034 4066 5547 5579 2306 Size: 32 1696 32 1481 32 2692 Fragments arranged by size: 2692 1696 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PstI C_TGCA'G Cuts at: 830 2313 3317 830 Size: 1483 1004 3478 Fragments arranged by size: 3478 1483 1004 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 2340 2692 2340 Size: 352 5613 SacI G_AGCT'C Cuts at: 2945 2945 Size: 5965 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 4213 5925 4213 Size: 1712 4253 SfcI C'TryA_G Cuts at: 826 1045 2309 2418 2521 3313 4448 5129 5320 Size: 219 1264 109 103 792 1135 681 191 Cuts at: 5320 826 Size: 1471 Fragments arranged by size: 1471 1264 1135 792 681 219 191 109 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 3292 1872 Size: 1420 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2136 3019 4069 4970 5232 820 Size: 197 926 193 883 1050 901 262 1553 Fragments arranged by size: 1553 1050 926 901 883 262 197 193 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 2592 3323 1132 Size: 1460 731 3774 Fragments arranged by size: 3774 1460 731 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 3404 513 Size: 552 2339 3074 Fragments arranged by size: 3074 2339 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3992 4009 4162 4347 4792 325 Size: 1255 2412 17 153 185 445 1498 Fragments arranged by size: 2412 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4211 5923 96 Size: 274 80 33 51 1443 2234 1712 138 Fragments arranged by size: 2234 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5925 5957 5925 Size: 32 5933 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 2191 3301 2191 Size: 1110 4855 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 3290 1870 Size: 1420 4545 XmnI GAAnn'nnTTC Cuts at: 4094 4094 Size: 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI