################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AhdI AlwNI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KasI KpnI MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PaeR7I PflMI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SalI SbfI ScaI SfcI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 330 GAATTT [GA]^AATT[CT] AcyI 589 GACGCC G[GA]^CG[CT]C AgeI 316 ACCGGT A^CCGGT Alw44I 202 GTGCAC G^TGCAC AlwI 192 GGATC GGATC(4/5) ApaLI 202 GTGCAC G^TGCAC ApoI 330 GAATTT [GA]^AATT[CT] AspI 382 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 438 TGGCCA TGG^CCA BanI 319 GGTGCC G^G[CT][GA]CC BanII 17 GGGCTC G[GA]GC[CT]^C BbvI 418 GCTGC GCAGC(8/12) BfaI 340 CTAG C^TAG BsaHI 589 GACGCC G[GA]^CG[CT]C BspHI 500 TCATGA T^CATGA BspMI 206 ACCTGC ACCTGC(4/8) BsrBI 472 GAGCGG CCGCTC(-3/-3) BsrFI 316 ACCGGT [GA]^CCGG[CT] Cfr10I 316 ACCGGT [GA]^CCGG[CT] DraIII 196 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 95 CCGCGG C^C[GA][CT]GG EaeI 438 TGGCCA [CT]^GGCC[GA] EarI 54 GAAGAG CTCTTC(1/4) EcoT14I 323 CCAAGG C^C[AT][AT]GG HgaI 589 GACGC GACGC(5/10) Hin1I 589 GACGCC G[GA]^CG[CT]C HphI 314 TCACC GGTGA(8/7) Ksp632I 54 GAAGAG CTCTTC(1/4) KspI 95 CCGCGG CCGC^GG MaeI 340 CTAG C^TAG MluNI 438 TGGCCA TGG^CCA MscI 438 TGGCCA TGG^CCA MslI 570 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PacI 244 TTAATTAA TTAAT^TAA PflFI 382 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 316 ACCGGT A^CCGGT PshAI 273 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 406 CAGCTG CAG^CTG RcaI 500 TCATGA T^CATGA SacII 95 CCGCGG CCGC^GG SapI 54 GAAGAGC GCTCTTC(1/4) SexAI 383 ACCTGGT A^CC[AT]GGT SgrAI 315 CACCGGTG C[GA]^CCGG[CT]G StyI 323 CCAAGG C^C[AT][AT]GG TaqI 551 TCGA T^CGA TseI 418 GCTGC G^C[AT]GC TspRI 600 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 382 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 551 TCGA T^CGA XcmI 374 CCAAACCCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Aor51HI 84 AGCGCT AGC^GCT 446 AGCGCT AvaII 42 GGACC G^G[AT]CC 387 GGTCC BpmI 397 CTCCAG CTGGAG(16/14) 31 CTGGAG DdeI 157 CTGAG C^T[ACGT]AG 584 CTGAG DpnII 134 GATC ^GATC 193 GATC Eco47III 84 AGCGCT AGC^GCT 446 AGCGCT MaeII 185 ACGT A^CGT 362 ACGT MboI 134 GATC ^GATC 193 GATC MboII 54 GAAGA GAAGA(8/7) 525 GAAGA MseI 244 TTAA T^TAA 248 TTAA NdeII 134 GATC ^GATC 193 GATC NlaIV 122 GGTTCC GG[ACGT]^[ACGT]CC 319 GGTGCC Sau3AI 134 GATC ^GATC 193 GATC Tru9I 244 TTAA T^TAA 248 TTAA Tsp45I 182 GTGAC ^GT[GC]AC 271 GTGAC Tsp509I 246 AATT ^AATT 331 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccII AciI AluI AspHI BcnI BmyI BsaJI BsaWI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII Sau96I ScrFI SfaNI