################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MamI MboII MluI MluNI MscI MslI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 515 TCCGGA T^CCGGA AciI 498 GCGG CCGC(-3/-1) AclI 542 AACGTT AA^CGTT AflIII 151 ACATGT A^C[GA][CT]GT AvaII 47 GGTCC G^G[AT]CC BanII 389 GGGCTC G[GA]GC[CT]^C BcnI 50 CCCGG CC^[GC]GG BfaI 10 CTAG C^TAG BglII 308 AGATCT A^GATCT BmyI 389 GGGCTC G[AGT]GC[ACT]^C BsaI 37 GGTCTC GGTCTC(1/5) BsaJI 228 CCAGGG C^C[ACGT][ACGT]GG BsaWI 515 TCCGGA [AT]^CCGG[AT] BseAI 515 TCCGGA T^CCGGA Bsp1286I 389 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 148 TGTACA T^GTACA BspEI 515 TCCGGA T^CCGGA BspLU11I 151 ACATGT A^CATGT BsrGI 148 TGTACA T^GTACA BsrI 382 ACTGG ACTGG(1/-1) BstNI 228 CCAGG CC^[AT]GG BstYI 308 AGATCT [GA]^GATC[CT] Cac8I 9 GCTAGC GC[ACGT]^[ACGT]GC Cfr13I 47 GGTCC G^G[ACGT]CC DdeI 349 CTGAG C^T[ACGT]AG DraII 46 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 46 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 228 CCAGG ^CC[AT]GG FauI 498 GCGGG CCCGC(4/6) HgaI 67 GCGTC GACGC(5/10) HindIII 181 AAGCTT A^AGCTT MaeI 10 CTAG C^TAG MaeII 543 ACGT A^CGT MfeI 371 CAATTG C^AATTG MflI 308 AGATCT [GA]^GATC[CT] MroI 515 TCCGGA T^CCGGA MunI 371 CAATTG C^AATTG MvaI 228 CCAGG CC^[AT]GG MwoI 58 GCAGCCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 50 CCCGG CC^[GC]GG NheI 9 GCTAGC G^CTAGC NspI 151 ACATGT [GA]CATG^[CT] PflMI 305 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 46 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 31 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 542 AACGTT AA^CGTT PstI 482 CTGCAG CTGCA^G Sau96I 47 GGTCC G^G[ACGT]CC SfaNI 265 GATGC GCATC(5/9) SfcI 482 CTGCAG C^T[GA][CT]AG SspBI 148 TGTACA T^GTACA SspI 472 AATATT AAT^ATT TaqI 76 TCGA T^CGA Tsp45I 29 GTGAC ^GT[GC]AC TthHB8I 76 TCGA T^CGA Van91I 305 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 308 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 238 AAATTT [GA]^AATT[CT] 453 GAATTT AfaI 149 GTAC GT^AC 270 GTAC AlwI 172 GATCC GGATC(4/5) 463 GATCC ApoI 238 AAATTT [GA]^AATT[CT] 453 GAATTT BanI 279 GGTGCC G^G[CT][GA]CC 314 GGCACC BbvI 58 GCAGC GCAGC(8/12) 481 GCTGC BsmFI 500 GGGAC GGGAC(10/14) 48 GTCCC BssKI 50 CCCGG ^CC[ACGT]GG 228 CCAGG BstF5I 411 GGATG GGATG(2/0) 528 GGATG Fnu4HI 58 GCAGC GC^[ACGT]GC 481 GCTGC FokI 411 GGATG GGATG(9/13) 528 GGATG HapII 51 CCGG C^CGG 516 CCGG HinfI 73 GACTC G^A[ACGT]TC 78 GACTC HpaII 51 CCGG C^CGG 516 CCGG ItaI 58 GCAGC GC^[ACGT]GC 481 GCTGC MspI 51 CCGG C^CGG 516 CCGG NlaIII 152 CATG CATG^ 276 CATG PleI 73 GACTC GAGTC(4/5) 78 GACTC RsaI 149 GTAC GT^AC 270 GTAC ScrFI 50 CCCGG CC^[ACGT]GG 228 CCAGG TseI 58 GCAGC G^C[AT]GC 481 GCTGC TspRI 381 CACTG CA[GC]TG[ACGT][ACGT]^ 467 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BsiYI BslI BsmAI DpnII MaeIII MboI MnlI MseI NdeII NlaIV Sau3AI Tru9I Tsp509I