Restriction map for
pFN21AB6295
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI ApaI AscI AseI
AsnI Asp718I AspI AviII BalI
BamHI BbeI BbrPI BcgI BclI
BfrI BglII BsaAI BsaBI BsaHI
BsiWI BsmBI BsmI BspDI BspLU11I
BsrDI BsrFI BssHII BstAPI BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraI DraIII DrdI EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I KasI KpnI MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 1932 TCCGGA T^CCGGA
AhdI 1843 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1184 GTGCAC G^TGCAC
AocI 243 CCTGAGG CC^T[ACGT]AGG
Aor51HI 164 AGCGCT AGC^GCT
ApaLI 1184 GTGCAC G^TGCAC
AspEI 1843 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1184 GTGCAC G[AT]GC[AT]^C
BbvCI 1999 GCTGAGG CCTCAGC(-5/-2)
BciVI 817 GGATAC GTATCC(6/5)
BglI 316 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1932 TCCGGA [AT]^CCGG[AT]
BseAI 1932 TCCGGA T^CCGGA
BsgI 2175 CTGCAC GTGCAG(16/14)
BsiEI 14 CGGCCG CG[GA][CT]^CG
BsiHKAI 1184 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1986 TGTACA T^GTACA
BspEI 1932 TCCGGA T^CCGGA
BspHI 1353 TCATGA T^CATGA
BsrGI 1986 TGTACA T^GTACA
BssSI 1317 CTCGTG CACGAG(-5/-1)
Bst1107I 683 GTATAC GTA^TAC
BstXI 896 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 683 GTATAC GTA^TAC
Bsu36I 243 CCTGAGG CC^T[ACGT]AGG
DsaI 11 CCGCGG C^C[GA][CT]GG
EaeI 14 CGGCCG [CT]^GGCC[GA]
EagI 14 CGGCCG C^GGCCG
Eam1105I 1843 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 14 CGGCCG C^GGCCG
Eco47III 164 AGCGCT AGC^GCT
Eco52I 14 CGGCCG C^GGCCG
Eco81I 243 CCTGAGG CC^T[ACGT]AGG
EcoRI 1423 GAATTC G^AATTC
HaeII 164 AGCGCT [GA]GCGC^[CT]
HgaI 2164 GACGC GACGC(5/10)
HindIII 2285 AAGCTT A^AGCTT
HpaI 1782 GTTAAC GTT^AAC
KspI 11 CCGCGG CCGC^GG
MaeII 1786 ACGT A^CGT
MroI 1932 TCCGGA T^CCGGA
NotI 13 GCGGCCGC GC^GGCCGC
NruI 111 TCGCGA TCG^CGA
PaeR7I 742 CTCGAG C^TCGAG
PflMI 1223 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1353 TCATGA T^CATGA
SacII 11 CCGCGG CCGC^GG
SalI 867 GTCGAC G^TCGAC
SexAI 569 ACCAGGT A^CC[AT]GGT
SspBI 1986 TGTACA T^GTACA
Van91I 1223 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1219 CCATCCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 742 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 1105 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1419 GAAAGAATTC
AvaI 742 CTCGAG C^[CT]CG[GA]G
2064 CCCGAG
AvrII 885 CCTAGG C^CTAGG
1074 CCTAGG
BanI 2125 GGTGCC G^G[CT][GA]CC
2325 GGCACC
BlnI 885 CCTAGG C^CTAGG
1074 CCTAGG
BlpI 535 GCTCAGC GC^T[ACGT]AGC
1444 GCTGAGC
Bpu1102I 535 GCTCAGC GC^T[ACGT]AGC
1444 GCTGAGC
BsaI 258 GAGACC GGTCTC(1/5)
287 GGTCTC
BseRI 2002 GAGGAG GAGGAG(10/8)
2378 GAGGAG
BsoBI 742 CTCGAG C^[CT]CG[GA]G
2064 CCCGAG
BspMI 1087 GCAGGT ACCTGC(4/8)
1827 GCAGGT
BsrBI 17 CCGCTC CCGCTC(-3/-3)
136 GAGCGG
BsrI 896 CCAGT ACTGG(1/-1)
1744 CCAGT
BstYI 116 AGATCC [GA]^GATC[CT]
178 AGATCC
CelII 535 GCTCAGC GC^T[ACGT]AGC
1444 GCTGAGC
FauI 10 CCCGC CCCGC(4/6)
98 CCCGC
HincII 867 GTCGAC GT[CT]^[GA]AC
1782 GTTAAC
HindII 867 GTCGAC GT[CT]^[GA]AC
1782 GTTAAC
MflI 116 AGATCC [GA]^GATC[CT]
178 AGATCC
MslI 219 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1333 CATAGTGATG
PstI 311 CTGCAG CTGCA^G
2144 CTGCAG
SfaNI 2218 GATGC GCATC(5/9)
391 GCATC
SfcI 311 CTGCAG C^T[GA][CT]AG
2144 CTGCAG
XhoII 116 AGATCC [GA]^GATC[CT]
178 AGATCC
XmnI 1105 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1419 GAAAGAATTC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I