Restriction map for
pFN21AB6295
(Linear) (Six-base) MAPSORT of: KIBB6295.seq Check: 8850 from: 1 to: 2424
KIBB6295.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1078 2424
Size: 1078 1346
AccI GT'mk_AC
Cuts at: 0 684 868 1613 1837 2424
Size: 684 184 745 224 587
Fragments arranged by size:
745 684 587 224 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 742 1076 2424
Size: 725 17 334 1348
Fragments arranged by size:
1348 725 334 17
AfeI AGC'GCT
Cuts at: 0 166 2424
Size: 166 2258
AhdI GACnn_n'nnGTC
Cuts at: 0 1848 2424
Size: 1848 576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 475 2190 2424
Size: 475 1715 234
Fragments arranged by size:
1715 475 234
AlwNI CAG_nnn'CTG
Cuts at: 0 63 1573 1999 2424
Size: 63 1510 426 425
Fragments arranged by size:
1510 426 425 63
ApaLI G'TGCA_C
Cuts at: 0 1184 2424
Size: 1184 1240
ApoI r'AATT_y
Cuts at: 0 655 765 1121 1423 2424
Size: 655 110 356 302 1001
Fragments arranged by size:
1001 655 356 302 110
AvaI C'yCGr_G
Cuts at: 0 742 2064 2424
Size: 742 1322 360
Fragments arranged by size:
1322 742 360
AvrII C'CTAG_G
Cuts at: 0 885 1074 2424
Size: 885 189 1350
Fragments arranged by size:
1350 885 189
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 810 843 2424
Size: 810 33 1581
Fragments arranged by size:
1581 810 33
BanI G'GyrC_C
Cuts at: 0 2125 2325 2424
Size: 2125 200 99
Fragments arranged by size:
2125 200 99
BanII G_rGCy'C
Cuts at: 0 49 1451 1649 2424
Size: 49 1402 198 775
Fragments arranged by size:
1402 775 198 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 161 215 1064 1708 2168 2414 2424
Size: 161 54 849 644 460 246 10
Fragments arranged by size:
849 644 460 246 161 54 10
BbvCI CC'TCA_GC
Cuts at: 0 2000 2424
Size: 2000 424
BciVI GTATCCnnnnn_n'
Cuts at: 0 811 2424
Size: 811 1613
BglI GCCn_nnn'nGGC
Cuts at: 0 322 2424
Size: 322 2102
BlpI GC'TnA_GC
Cuts at: 0 536 1445 2424
Size: 536 909 979
Fragments arranged by size:
979 909 536
Bme1580I G_kGCm'C
Cuts at: 0 1188 2348 2424
Size: 1188 1160 76
Fragments arranged by size:
1188 1160 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 1738 2424
Size: 1738 686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 1154 2424
Size: 186 968 1270
Fragments arranged by size:
1270 968 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 619 1547 2424
Size: 154 465 928 877
Fragments arranged by size:
928 877 465 154
Bpu10I CC'TnA_GC
Cuts at: 0 1083 2000 2424
Size: 1083 917 424
Fragments arranged by size:
1083 917 424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1147 1234 2424
Size: 847 300 87 1190
Fragments arranged by size:
1190 847 300 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 252 293 2424
Size: 252 41 2131
Fragments arranged by size:
2131 252 41
BsaWI w'CCGG_w
Cuts at: 0 1932 2424
Size: 1932 492
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1191 1221 2424
Size: 1191 30 1203
Fragments arranged by size:
1203 1191 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2017 2393 2424
Size: 2017 376 31
Fragments arranged by size:
2017 376 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2160 2424
Size: 2160 264
BsiEI CG_ry'CG
Cuts at: 0 17 2424
Size: 17 2407
BsiHKAI G_wGCw'C
Cuts at: 0 1188 2424
Size: 1188 1236
Bsp1286I G_dGCh'C
Cuts at: 0 49 1188 1451 1649 2348 2424
Size: 49 1139 263 198 699 76
Fragments arranged by size:
1139 699 263 198 76 49
BspEI T'CCGG_A
Cuts at: 0 1932 2424
Size: 1932 492
BspHI T'CATG_A
Cuts at: 0 1353 2424
Size: 1353 1071
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1078 1818 2424
Size: 1078 740 606
Fragments arranged by size:
1078 740 606
BsrBI CCG'CTC
Cuts at: 0 19 138 2424
Size: 19 119 2286
Fragments arranged by size:
2286 119 19
BsrGI T'GTAC_A
Cuts at: 0 1986 2424
Size: 1986 438
BssSI C'ACGA_G
Cuts at: 0 1317 2424
Size: 1317 1107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 903 2424
Size: 903 1521
BstYI r'GATC_y
Cuts at: 0 116 178 2424
Size: 116 62 2246
Fragments arranged by size:
2246 116 62
BstZ17I GTA'TAC
Cuts at: 0 685 2424
Size: 685 1739
Bsu36I CC'TnA_GG
Cuts at: 0 244 2424
Size: 244 2180
BtgI C'CryG_G
Cuts at: 0 11 2424
Size: 11 2413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1676 2424
Size: 1676 748
BtsI GCAGTG_nn'
Cuts at: 0 1061 1286 1862 1920 2424
Size: 1061 225 576 58 504
Fragments arranged by size:
1061 576 504 225 58
EaeI y'GGCC_r
Cuts at: 0 14 2424
Size: 14 2410
EagI C'GGCC_G
Cuts at: 0 14 2424
Size: 14 2410
EarI CTCTTCn'nnn_
Cuts at: 0 248 497 635 842 885 1289 1496 1817
Size: 248 249 138 207 43 404 207 321
Cuts at: 1817 2328 2424
Size: 511 96
Fragments arranged by size:
511 404 321 249 248 207 207 138
96 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 2424
Size: 82 2342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 619 725 742 1076 1547 2424
Size: 154 465 106 17 334 471 877
Fragments arranged by size:
877 471 465 334 154 106 17
EcoO109I rG'GnC_Cy
Cuts at: 0 717 826 881 1377 2424
Size: 717 109 55 496 1047
Fragments arranged by size:
1047 717 496 109 55
EcoRI G'AATT_C
Cuts at: 0 1423 2424
Size: 1423 1001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 576 2424
Size: 576 1848
HaeII r_GCGC'y
Cuts at: 0 168 2424
Size: 168 2256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 271 303 330 362 1033 1065
Size: 205 32 34 32 27 32 671 32
Cuts at: 1065 1191 1223 1834 1866 1869 1901 2305 2337
Size: 126 32 611 32 3 32 404 32
Cuts at: 2337 2424
Size: 87
Fragments arranged by size:
671 611 404 205 126 87 34 32
32 32 32 32 32 32 32 27
3
HincII GTy'rAC
Cuts at: 0 869 1784 2424
Size: 869 915 640
Fragments arranged by size:
915 869 640
HindIII A'AGCT_T
Cuts at: 0 2285 2424
Size: 2285 139
HpaI GTT'AAC
Cuts at: 0 1784 2424
Size: 1784 640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 821 1210 1247 1934 2424
Size: 795 26 389 37 687 490
Fragments arranged by size:
795 687 490 389 37 26
MslI CAynn'nnrTG
Cuts at: 0 223 1337 2424
Size: 223 1114 1087
Fragments arranged by size:
1114 1087 223
MspA1I CmG'CkG
Cuts at: 0 13 359 615 1444 1999 2424
Size: 13 346 256 829 555 425
Fragments arranged by size:
829 555 425 346 256 13
NotI GC'GGCC_GC
Cuts at: 0 14 2424
Size: 14 2410
NruI TCG'CGA
Cuts at: 0 113 2424
Size: 113 2311
NspI r_CATG'y
Cuts at: 0 222 300 462 2424
Size: 222 78 162 1962
Fragments arranged by size:
1962 222 162 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 1229 2424
Size: 1229 1195
PpuMI rG'GwC_Cy
Cuts at: 0 826 881 1377 2424
Size: 826 55 496 1047
Fragments arranged by size:
1047 826 496 55
PstI C_TGCA'G
Cuts at: 0 315 2148 2424
Size: 315 1833 276
Fragments arranged by size:
1833 315 276
PvuII CAG'CTG
Cuts at: 0 615 1444 1999 2424
Size: 615 829 555 425
Fragments arranged by size:
829 615 555 425
SacII CC_GC'GG
Cuts at: 0 14 2424
Size: 14 2410
SalI G'TCGA_C
Cuts at: 0 867 2424
Size: 867 1557
SanDI GG'GwC_CC
Cuts at: 0 881 2424
Size: 881 1543
SapI GCTCTTCn'nnn_
Cuts at: 0 885 1289 1817 2424
Size: 885 404 528 607
Fragments arranged by size:
885 607 528 404
SexAI A'CCwGG_T
Cuts at: 0 569 2424
Size: 569 1855
SfcI C'TryA_G
Cuts at: 0 311 2144 2424
Size: 311 1833 280
Fragments arranged by size:
1833 311 280
SmlI C'TyrA_G
Cuts at: 0 742 862 1126 1213 2424
Size: 742 120 264 87 1211
Fragments arranged by size:
1211 742 264 120 87
StyI C'CwwG_G
Cuts at: 0 822 885 1074 1451 2424
Size: 822 63 189 377 973
Fragments arranged by size:
973 822 377 189 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 1171 2424
Size: 322 849 1253
Fragments arranged by size:
1253 849 322
TatI w'GTAC_w
Cuts at: 0 1155 1471 1606 1986 2236 2424
Size: 1155 316 135 380 250 188
Fragments arranged by size:
1155 380 316 250 188 135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1226 2424
Size: 1226 1198
XhoI C'TCGA_G
Cuts at: 0 742 2424
Size: 742 1682
XmnI GAAnn'nnTTC
Cuts at: 0 1109 1423 2424
Size: 1109 314 1001
Fragments arranged by size:
1109 1001 314
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaLI
ApoI AvaI AvrII BaeI BanI BanII BbsI BbvCI
BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrGI BssSI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII HpaI MmeI MslI MspA1I NotI NruI
NspI PflMI PpuMI PstI PvuII SacII SalI SanDI
SapI SexAI SfcI SmlI StyI TaqII TatI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
ApaI AscI AseI AsiSI BamHI BbeI BcgI BclI
BfrBI BglII BmgBI BmtI BsaAI BsaBI BsaHI BseYI
BsiWI BsmI BsmBI BsrDI BsrFI BssHII BstAPI BstBI
BstEII ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRV FseI FspI FspAI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XmaI ZraI