################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 184 GACGTC GACGT^C AccII 133 CGCG CG^CG AcyI 184 GACGTC G[GA]^CG[CT]C AluI 60 AGCT AG^CT BbvCI 9 GCTGAGG CCTCAGC(-5/-2) BbvI 61 GCTGC GCAGC(8/12) BcnI 209 CCCGG CC^[GC]GG BsaHI 184 GACGTC G[GA]^CG[CT]C BseRI 165 GAGGAG GAGGAG(10/8) BsiEI 159 CGGTCG CG[GA][CT]^CG BspMI 217 ACCTGC ACCTGC(4/8) BsrI 128 CCAGT ACTGG(1/-1) BstF5I 255 GGATG GGATG(2/0) BstUI 133 CGCG CG^CG BstYI 92 AGATCC [GA]^GATC[CT] DdeI 10 CTGAG C^T[ACGT]AG DraII 13 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 86 CCATGG C^C[GA][CT]GG EcoO109I 13 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 86 CCATGG C^C[AT][AT]GG FokI 255 GGATG GGATG(9/13) HapII 210 CCGG C^CGG Hin1I 184 GACGTC G[GA]^CG[CT]C HpaII 210 CCGG C^CGG MaeII 185 ACGT A^CGT MflI 92 AGATCC [GA]^GATC[CT] MspI 210 CCGG C^CGG MvnI 133 CGCG CG^CG NciI 209 CCCGG CC^[GC]GG NcoI 86 CCATGG C^CATGG NlaIII 87 CATG CATG^ NlaIV 20 GGCTCC GG[ACGT]^[ACGT]CC SfaNI 36 GATGC GCATC(5/9) StuI 142 AGGCCT AGG^CCT StyI 86 CCATGG C^C[AT][AT]GG TseI 61 GCTGC G^C[AT]GC XhoII 92 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 71 GTAC GT^AC 123 GTAC AlwI 93 GATCC GGATC(4/5) 137 GGATC Cac8I 29 GCCTGC GC[ACGT]^[ACGT]GC 200 GCCTGC DpnII 93 GATC ^GATC 138 GATC FauI 54 GCGGG CCCGC(4/6) 134 GCGGG Fnu4HI 61 GCTGC GC^[ACGT]GC 152 GCCGC ItaI 61 GCTGC GC^[ACGT]GC 152 GCCGC MboI 93 GATC ^GATC 138 GATC NdeII 93 GATC ^GATC 138 GATC RsaI 71 GTAC GT^AC 123 GTAC Sau3AI 93 GATC ^GATC 138 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI BsaJI BsiYI BslI BsmFI BssKI BstNI Cfr13I EcoRII HaeIII MnlI MvaI MwoI Sau96I ScrFI