(Circular) (Six-base) MAPSORT of: pFN21AE3007.seq Check: 9208 from: 1 to: 4966 pFN21AE3007 4966 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2193 2776 278 Size: 53 83 186 936 657 583 2468 Fragments arranged by size: 2468 936 657 583 186 83 53 AccI GT'mk_AC Cuts at: 1937 2309 1937 Size: 372 4594 Acc65I G'GTAC_C Cuts at: 2287 2287 Size: 4966 AclI AA'CG_TT Cuts at: 1563 3093 3466 1563 Size: 1530 373 3063 Fragments arranged by size: 3063 1530 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3029 4413 4681 1632 Size: 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4769 AflIII A'CryG_T Cuts at: 1203 3865 4842 1203 Size: 2662 977 1327 Fragments arranged by size: 2662 1327 977 AgeI A'CCGG_T Cuts at: 3797 3797 Size: 4966 AhdI GACnn_n'nnGTC Cuts at: 3695 3695 Size: 4966 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4966 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4966 AlwNI CAG_nnn'CTG Cuts at: 2709 4281 2709 Size: 1572 3394 ApaLI G'TGCA_C Cuts at: 3023 4179 3023 Size: 1156 3810 ApoI r'AATT_y Cuts at: 1493 2275 2501 2653 4762 1493 Size: 782 226 152 2109 1697 Fragments arranged by size: 2109 1697 782 226 152 AseI AT'TA_AT Cuts at: 160 1032 3520 160 Size: 872 2488 1606 Fragments arranged by size: 2488 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4966 AvaI C'yCGr_G Cuts at: 1870 1943 2291 1870 Size: 73 348 4545 Fragments arranged by size: 4545 348 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2119 2152 2119 Size: 33 4933 BamHI G'GATC_C Cuts at: 2296 2663 2296 Size: 367 4599 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2287 3742 618 Size: 325 217 627 500 1455 1842 Fragments arranged by size: 1842 1455 627 500 325 217 BanII G_rGCy'C Cuts at: 1391 2285 1391 Size: 894 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3995 BbvCI CC'TCA_GC Cuts at: 2015 2015 Size: 4966 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3157 3191 1903 Size: 34 1220 34 3678 Fragments arranged by size: 3678 1220 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2858 4068 2858 Size: 1210 3756 BclI T'GATC_A Cuts at: 1548 1548 Size: 4966 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3577 136 Size: 107 122 71 631 605 1905 1525 Fragments arranged by size: 1905 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4961 4961 Size: 4966 BlpI GC'TnA_GC Cuts at: 2383 2383 Size: 4966 Bme1580I G_kGCm'C Cuts at: 1790 3027 4183 1790 Size: 1237 1156 2573 Fragments arranged by size: 2573 1237 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4966 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2697 3655 442 Size: 416 53 83 399 24 1280 958 1753 Fragments arranged by size: 1753 1280 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3626 1239 Size: 2387 2579 Bpu10I CC'TnA_GC Cuts at: 2015 2015 Size: 4966 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3091 3956 4254 3091 Size: 865 298 3803 Fragments arranged by size: 3803 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3629 882 Size: 617 2130 2219 Fragments arranged by size: 2219 2130 617 BsaAI yAC'GTr Cuts at: 493 1204 2757 493 Size: 711 1553 2702 Fragments arranged by size: 2702 1553 711 BsaBI GATnn'nnATC Cuts at: 4960 4960 Size: 4966 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 2773 3155 3790 Size: 53 83 186 936 657 583 382 635 Cuts at: 3790 275 Size: 1451 Fragments arranged by size: 1451 936 657 635 583 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3399 3797 4071 4218 4629 3399 Size: 398 274 147 411 3736 Fragments arranged by size: 3736 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4741 Fragments arranged by size: 4741 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2185 1186 Size: 999 3967 BseYI C'CCAG_C Cuts at: 2044 4169 4665 2044 Size: 2125 496 2345 Fragments arranged by size: 2345 2125 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4393 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2167 2416 3177 4205 1540 Size: 275 186 166 249 761 1028 2301 Fragments arranged by size: 2301 1028 761 275 249 186 166 BsiHKAI G_wGCw'C Cuts at: 3027 3112 4183 3027 Size: 85 1071 3810 Fragments arranged by size: 3810 1071 85 BsmI GAATG_Cn' Cuts at: 2477 2570 2477 Size: 93 4873 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 3027 3112 4183 1391 Size: 399 495 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 399 85 BspEI T'CCGG_A Cuts at: 4629 4629 Size: 4966 BspHI T'CATG_A Cuts at: 2855 4585 2855 Size: 1730 3236 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 2321 844 Size: 1387 90 3489 Fragments arranged by size: 3489 1387 90 BsrBI CCG'CTC Cuts at: 1394 1823 2853 1394 Size: 429 1030 3507 Fragments arranged by size: 3507 1030 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3461 3635 65 Size: 1183 2213 174 1396 Fragments arranged by size: 2213 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3610 3797 1700 Size: 110 1800 187 2869 Fragments arranged by size: 2869 1800 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4966 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4966 BssSI C'ACGA_G Cuts at: 3026 4038 3026 Size: 1012 3954 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4966 BstBI TT'CG_AA Cuts at: 3770 3770 Size: 4966 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4966 BstYI r'GATC_y Cuts at: 1972 2097 2296 2663 3048 3065 4517 4603 4615 Size: 125 199 367 385 17 1452 86 12 Cuts at: 4615 4961 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 199 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2091 513 Size: 552 1026 3388 Fragments arranged by size: 3388 1026 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4227 Fragments arranged by size: 4227 683 56 BtsI GCAGTG_nn' Cuts at: 2482 3276 3296 2482 Size: 794 20 4152 Fragments arranged by size: 4152 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4966 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4931 DraI TTT'AAA Cuts at: 2270 2270 Size: 4966 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3973 809 Size: 507 2657 1802 Fragments arranged by size: 2657 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2413 3302 4931 Size: 54 1425 50 275 18 583 889 1629 Cuts at: 4931 8 Size: 43 Fragments arranged by size: 1629 1425 889 583 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2413 1537 Size: 275 601 4090 Fragments arranged by size: 4090 601 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2896 1353 Size: 1543 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3553 3927 4073 387 Size: 1064 138 1964 374 146 1280 Fragments arranged by size: 1964 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3029 3626 4413 4681 1239 Size: 393 1397 597 787 268 1524 Fragments arranged by size: 1524 1397 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2019 2019 Size: 4966 EcoRI G'AATT_C Cuts at: 2275 2653 2275 Size: 378 4588 FspI TGC'GCA Cuts at: 3472 3472 Size: 4966 HaeII r_GCGC'y Cuts at: 1678 4113 1678 Size: 2435 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3607 3639 3681 3713 1373 Size: 32 135 32 2035 32 42 32 2626 Fragments arranged by size: 2626 2035 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2310 2556 669 Size: 988 281 372 246 3079 Fragments arranged by size: 3079 988 372 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4966 HpaI GTT'AAC Cuts at: 2556 2556 Size: 4966 KpnI G_GTAC'C Cuts at: 2291 2291 Size: 4966 MfeI C'AATT_G Cuts at: 2565 2565 Size: 4966 MluI A'CGCG_T Cuts at: 4842 4842 Size: 4966 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2147 4080 4264 1305 Size: 842 1933 184 2007 Fragments arranged by size: 2007 1933 842 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3144 Fragments arranged by size: 3144 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2924 3283 3442 518 Size: 648 843 915 359 159 2042 Fragments arranged by size: 2042 915 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2382 3059 4207 4452 1606 Size: 776 677 1148 245 2120 Fragments arranged by size: 2120 1148 776 677 245 NcoI C'CATG_G Cuts at: 513 1065 2091 513 Size: 552 1026 3388 Fragments arranged by size: 3388 1026 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4966 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4966 NotI GC'GGCC_GC Cuts at: 2413 2413 Size: 4966 NspI r_CATG'y Cuts at: 1132 1229 2324 3869 1132 Size: 97 1095 1545 2229 Fragments arranged by size: 2229 1545 1095 97 PasI CC'CwG_GG Cuts at: 1348 2182 1348 Size: 834 4132 PciI A'CATG_T Cuts at: 3865 3865 Size: 4966 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4966 PmeI GTTT'AAAC Cuts at: 2270 2270 Size: 4966 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3035 3067 4548 4580 3035 Size: 32 1481 32 3421 Fragments arranged by size: 3421 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4966 PsiI TTA'TAA Cuts at: 2536 2536 Size: 4966 PstI C_TGCA'G Cuts at: 830 2318 830 Size: 1488 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4966 SalI G'TCGA_C Cuts at: 1936 2308 1936 Size: 372 4594 SbfI CC_TGCA'GG Cuts at: 2318 2318 Size: 4966 ScaI AGT'ACT Cuts at: 3214 4926 3214 Size: 1712 3254 SfcI C'TryA_G Cuts at: 826 1045 2314 3449 4130 4321 826 Size: 219 1269 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4966 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4966 SmaI CCC'GGG Cuts at: 1872 2293 1872 Size: 421 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3070 3971 4233 820 Size: 197 926 1127 901 262 1553 Fragments arranged by size: 1553 1127 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2757 493 Size: 2264 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4966 SphI G_CATG'C Cuts at: 1132 2324 1132 Size: 1192 3774 SspI AAT'ATT Cuts at: 5 52 2890 5 Size: 47 2838 2081 Fragments arranged by size: 2838 2081 47 StuI AGG'CCT Cuts at: 1517 2149 1517 Size: 632 4334 StyI C'CwwG_G Cuts at: 513 1065 2091 2405 513 Size: 552 1026 314 3074 Fragments arranged by size: 3074 1026 552 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 2993 3010 3163 3348 3793 325 Size: 1255 573 840 17 153 185 445 1498 Fragments arranged by size: 1498 1255 840 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3212 4924 96 Size: 274 80 33 51 1443 1235 1712 138 Fragments arranged by size: 1712 1443 1235 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4926 4958 4926 Size: 32 4934 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4966 XbaI T'CTAG_A Cuts at: 2302 2302 Size: 4966 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4966 XmaI C'CCGG_G Cuts at: 1870 2291 1870 Size: 421 4545 XmnI GAAnn'nnTTC Cuts at: 3095 3095 Size: 4966 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2191 2774 276 Size: 53 83 186 936 657 583 2468 Fragments arranged by size: 2468 936 657 583 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI