################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 372 GTATAC GT^[AC][GT]AC AccII 346 CGCG CG^CG AlwNI 548 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 509 GGGCCC GGGCC^C AspHI 70 GAGCTC G[AT]GC[AT]^C AvaI 73 CTCGAG C^[CT]CG[GA]G AvaII 303 GGTCC G^G[AT]CC BbvI 400 GCAGC GCAGC(8/12) BcgI 390 CGATGATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 121 CTAG C^TAG BsaI 360 GGTCTC GGTCTC(1/5) BsiHKAI 70 GAGCTC G[AT]GC[AT]^C BsoBI 73 CTCGAG C^[CT]CG[GA]G BspMI 183 ACCTGC ACCTGC(4/8) Bst1107I 372 GTATAC GTA^TAC BstUI 346 CGCG CG^CG BstXI 473 CCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 263 GGATCT [GA]^GATC[CT] BstZ17I 372 GTATAC GTA^TAC EarI 424 GAAGAG CTCTTC(1/4) EcoRV 161 GATATC GAT^ATC Fnu4HI 400 GCAGC GC^[ACGT]GC HaeII 25 AGCGCC [GA]GCGC^[CT] HgaI 344 GACGC GACGC(5/10) ItaI 400 GCAGC GC^[ACGT]GC Ksp632I 424 GAAGAG CTCTTC(1/4) MaeI 121 CTAG C^TAG MboII 424 GAAGA GAAGA(8/7) MflI 263 GGATCT [GA]^GATC[CT] MseI 527 TTAA T^TAA MslI 474 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 346 CGCG CG^CG NlaIII 147 CATG CATG^ PaeR7I 73 CTCGAG C^TCGAG PflMI 103 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 70 GAGCTC GAGCT^C SfaNI 331 GATGC GCATC(5/9) SfcI 419 CTGTAG C^T[GA][CT]AG TaqI 74 TCGA T^CGA Tru9I 527 TTAA T^TAA TseI 400 GCAGC G^C[AT]GC TthHB8I 74 TCGA T^CGA Van91I 103 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 470 CCACCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 73 CTCGAG C^TCGAG XhoII 263 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 10 GCGG CCGC(-3/-1) 19 GCGG AcsI 201 AAATTC [GA]^AATT[CT] 218 GAATTT AlwI 51 GGATC GGATC(4/5) 263 GGATC ApoI 201 AAATTC [GA]^AATT[CT] 218 GAATTT BanII 70 GAGCTC G[GA]GC[CT]^C 509 GGGCCC BmyI 70 GAGCTC G[AGT]GC[ACT]^C 509 GGGCCC BpmI 557 CTCCAG CTGGAG(16/14) 83 CTGGAG BsiYI 103 CCAGTGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCAGTTGCAGG BslI 103 CCAGTGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 499 CCAGTTGCAGG BsmBI 298 GAGACG CGTCTC(1/5) 342 GAGACG Bsp1286I 70 GAGCTC G[AGT]GC[ACT]^C 509 GGGCCC BstF5I 454 GGATG GGATG(2/0) 516 GGATG Cac8I 459 GCAAGC GC[ACGT]^[ACGT]GC 552 GCCTGC CfoI 26 GCGC GCG^C 347 GCGC DdeI 296 CTGAG C^T[ACGT]AG 325 CTTAG DpnII 52 GATC ^GATC 264 GATC FokI 454 GGATG GGATG(9/13) 516 GGATG HaeIII 78 GGCC GG^CC 510 GGCC HhaI 26 GCGC GCG^C 347 GCGC HinP1I 26 GCGC G^CGC 347 GCGC HinfI 239 GAATC G^A[ACGT]TC 466 GATTC HphI 12 GGTGA GGTGA(8/7) 285 TCACC MboI 52 GATC ^GATC 264 GATC NdeII 52 GATC ^GATC 264 GATC Sau3AI 52 GATC ^GATC 264 GATC SmlI 73 CTCGAG C^T[CT][GA]AG 365 CTCAAG TfiI 239 GAATC G^A[AT]TC 466 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BsaJI BsmAI BsrI BssKI BstNI Cfr13I DraII EcoO109I EcoRII MnlI MvaI MwoI NlaIV Sau96I ScrFI Tsp509I TspRI