(Circular) (Six-base) MAPSORT of: pFN21AE9281.seq Check: 9677 from: 1 to: 5332 pFN21AE9281 5332 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2197 2197 Size: 5332 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3142 278 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2378 2675 1937 Size: 441 297 4594 Fragments arranged by size: 4594 441 297 Acc65I G'GTAC_C Cuts at: 2653 2653 Size: 5332 AclI AA'CG_TT Cuts at: 1563 2631 3459 3832 1563 Size: 1068 828 373 3063 Fragments arranged by size: 3063 1068 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3395 4779 5047 1632 Size: 1763 1384 268 1917 Fragments arranged by size: 1917 1763 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5135 AflIII A'CryG_T Cuts at: 1203 4231 5208 1203 Size: 3028 977 1327 Fragments arranged by size: 3028 1327 977 AgeI A'CCGG_T Cuts at: 4163 4163 Size: 5332 AhdI GACnn_n'nnGTC Cuts at: 4061 4061 Size: 5332 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5332 AlwNI CAG_nnn'CTG Cuts at: 2558 3075 4647 2558 Size: 517 1572 3243 Fragments arranged by size: 3243 1572 517 ApaI G_GGCC'C Cuts at: 2518 2518 Size: 5332 ApaLI G'TGCA_C Cuts at: 3389 4545 3389 Size: 1156 4176 ApoI r'AATT_y Cuts at: 1493 2206 2223 2641 2867 3019 5128 1493 Size: 713 17 418 226 152 2109 1697 Fragments arranged by size: 2109 1697 713 418 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 3886 160 Size: 872 2854 1606 Fragments arranged by size: 2854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2657 1870 Size: 73 135 579 4545 Fragments arranged by size: 4545 579 135 73 BamHI G'GATC_C Cuts at: 2662 3029 2662 Size: 367 4965 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2653 4108 618 Size: 325 217 627 866 1455 1842 Fragments arranged by size: 1842 1455 866 627 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2518 2651 1391 Size: 688 439 133 4072 Fragments arranged by size: 4072 688 439 133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4361 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2384 2418 3523 3557 1903 Size: 34 50 34 363 34 1105 34 3678 Fragments arranged by size: 3678 1105 363 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3224 4434 3224 Size: 1210 4122 BclI T'GATC_A Cuts at: 1548 1548 Size: 5332 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3943 136 Size: 107 122 71 631 605 2271 1525 Fragments arranged by size: 2271 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5327 5327 Size: 5332 BlpI GC'TnA_GC Cuts at: 2749 2749 Size: 5332 Bme1580I G_kGCm'C Cuts at: 1790 2518 3393 4549 1790 Size: 728 875 1156 2573 Fragments arranged by size: 2573 1156 875 728 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5332 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2102 3063 4021 Size: 416 53 83 399 24 685 961 958 Cuts at: 4021 442 Size: 1753 Fragments arranged by size: 1753 961 958 685 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5332 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2547 3992 1239 Size: 870 438 1445 2579 Fragments arranged by size: 2579 1445 870 438 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2355 3457 4322 4620 2355 Size: 1102 865 298 3067 Fragments arranged by size: 3067 1102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2371 3995 882 Size: 617 872 1624 2219 Fragments arranged by size: 2219 1624 872 617 BsaAI yAC'GTr Cuts at: 493 1204 3123 493 Size: 711 1919 2702 Fragments arranged by size: 2702 1919 711 BsaBI GATnn'nnATC Cuts at: 5326 5326 Size: 5332 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3139 3521 4156 275 Size: 53 83 186 936 1606 382 635 1451 Fragments arranged by size: 1606 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3765 4163 4437 4584 4995 3765 Size: 398 274 147 411 4102 Fragments arranged by size: 4102 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5107 Fragments arranged by size: 5107 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5332 BseYI C'CCAG_C Cuts at: 4535 5031 4535 Size: 496 4836 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4759 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2782 3543 4571 1540 Size: 275 186 781 761 1028 2301 Fragments arranged by size: 2301 1028 781 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 3393 3478 4549 2079 Size: 1314 85 1071 2862 Fragments arranged by size: 2862 1314 1071 85 BsmI GAATG_Cn' Cuts at: 2843 2936 2843 Size: 93 5239 BsmBI CGTCTCn'nnnn_ Cuts at: 2297 2341 2297 Size: 44 5288 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2518 2651 3393 3478 4549 1391 Size: 399 289 439 133 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 289 133 85 BspEI T'CCGG_A Cuts at: 4995 4995 Size: 5332 BspHI T'CATG_A Cuts at: 3221 4951 3221 Size: 1730 3602 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2197 2687 844 Size: 1353 490 3489 Fragments arranged by size: 3489 1353 490 BsrBI CCG'CTC Cuts at: 1394 1823 3219 1394 Size: 429 1396 3507 Fragments arranged by size: 3507 1396 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3827 4001 65 Size: 1183 2579 174 1396 Fragments arranged by size: 2579 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3976 4163 1700 Size: 110 2166 187 2869 Fragments arranged by size: 2869 2166 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5332 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5332 BssSI C'ACGA_G Cuts at: 3392 4404 3392 Size: 1012 4320 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5332 BstBI TT'CG_AA Cuts at: 4136 4136 Size: 5332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5332 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2485 2011 Size: 474 4858 BstYI r'GATC_y Cuts at: 1972 2268 2662 3029 3414 3431 4883 4969 4981 Size: 296 394 367 385 17 1452 86 12 Cuts at: 4981 5327 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 394 385 367 346 296 86 17 12 BstZ17I GTA'TAC Cuts at: 2379 2379 Size: 5332 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4780 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2374 487 Size: 683 56 1148 3445 Fragments arranged by size: 3445 1148 683 56 BtsI GCAGTG_nn' Cuts at: 2848 3642 3662 2848 Size: 794 20 4518 Fragments arranged by size: 4518 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5332 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5297 DraI TTT'AAA Cuts at: 2636 2636 Size: 5332 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4339 809 Size: 507 3023 1802 Fragments arranged by size: 3023 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2779 3668 5297 Size: 54 1425 50 275 18 949 889 1629 Cuts at: 5297 8 Size: 43 Fragments arranged by size: 1629 1425 949 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2779 1537 Size: 275 967 4090 Fragments arranged by size: 4090 967 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2424 3262 1353 Size: 1071 838 3423 Fragments arranged by size: 3423 1071 838 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 3919 4293 4439 387 Size: 1064 138 450 1880 374 146 1280 Fragments arranged by size: 1880 1280 1064 450 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 2077 Size: 5332 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2547 3395 3992 4779 5047 1239 Size: 393 477 438 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 597 477 438 393 268 EcoO109I rG'GnC_Cy Cuts at: 2083 2514 2515 2083 Size: 431 1 4900 Fragments arranged by size: 4900 431 1 EcoRI G'AATT_C Cuts at: 2641 3019 2641 Size: 378 4954 EcoRV GAT'ATC Cuts at: 2168 2168 Size: 5332 FspI TGC'GCA Cuts at: 3838 3838 Size: 5332 HaeII r_GCGC'y Cuts at: 1678 2034 4479 1678 Size: 356 2445 2531 Fragments arranged by size: 2531 2445 356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2229 2261 3973 4005 4047 Size: 32 135 32 657 32 1712 32 42 Cuts at: 4047 4079 1373 Size: 32 2626 Fragments arranged by size: 2626 1712 657 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2676 2922 669 Size: 988 281 738 246 3079 Fragments arranged by size: 3079 988 738 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5332 HpaI GTT'AAC Cuts at: 2922 2922 Size: 5332 KpnI G_GTAC'C Cuts at: 2657 2657 Size: 5332 MfeI C'AATT_G Cuts at: 2931 2931 Size: 5332 MluI A'CGCG_T Cuts at: 5208 5208 Size: 5332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2395 4446 4630 1305 Size: 1090 2051 184 2007 Fragments arranged by size: 2051 2007 1090 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3510 Fragments arranged by size: 3510 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2483 3290 3649 3808 518 Size: 648 843 474 807 359 159 2042 Fragments arranged by size: 2042 843 807 648 474 359 159 MspA1I CmG'CkG Cuts at: 1606 2748 3425 4573 4818 1606 Size: 1142 677 1148 245 2120 Fragments arranged by size: 2120 1148 1142 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4780 NdeI CA'TA_TG Cuts at: 387 387 Size: 5332 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5332 NotI GC'GGCC_GC Cuts at: 2779 2779 Size: 5332 NspI r_CATG'y Cuts at: 1132 1229 2690 4235 1132 Size: 97 1461 1545 2229 Fragments arranged by size: 2229 1545 1461 97 PasI CC'CwG_GG Cuts at: 1348 2264 1348 Size: 916 4416 PciI A'CATG_T Cuts at: 4231 4231 Size: 5332 PflMI CCAn_nnn'nTGG Cuts at: 1108 2114 1108 Size: 1006 4326 PmeI GTTT'AAAC Cuts at: 2636 2636 Size: 5332 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3401 3433 4914 4946 3401 Size: 32 1481 32 3787 Fragments arranged by size: 3787 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5332 PsiI TTA'TAA Cuts at: 2902 2902 Size: 5332 PspOMI G'GGCC_C Cuts at: 2514 2514 Size: 5332 PspXI vC'TCGA_Gb Cuts at: 2078 2078 Size: 5332 PstI C_TGCA'G Cuts at: 830 2684 830 Size: 1854 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5332 SacI G_AGCT'C Cuts at: 2079 2079 Size: 5332 SalI G'TCGA_C Cuts at: 1936 2674 1936 Size: 738 4594 SbfI CC_TGCA'GG Cuts at: 2684 2684 Size: 5332 ScaI AGT'ACT Cuts at: 3580 5292 3580 Size: 1712 3620 SfcI C'TryA_G Cuts at: 826 1045 2424 2680 3815 4496 4687 826 Size: 219 1379 256 1135 681 191 1471 Fragments arranged by size: 1471 1379 1135 681 256 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5332 SmaI CCC'GGG Cuts at: 1872 2659 1872 Size: 787 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2370 3436 4337 4599 820 Size: 197 926 135 292 1066 901 262 1553 Fragments arranged by size: 1553 1066 926 901 292 262 197 135 SnaBI TAC'GTA Cuts at: 493 3123 493 Size: 2630 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5332 SphI G_CATG'C Cuts at: 1132 2690 1132 Size: 1558 3774 SspI AAT'ATT Cuts at: 5 52 3256 5 Size: 47 3204 2081 Fragments arranged by size: 3204 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5332 StyI C'CwwG_G Cuts at: 513 1065 2771 513 Size: 552 1706 3074 Fragments arranged by size: 3074 1706 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3359 3376 3529 3714 4159 325 Size: 1255 1779 17 153 185 445 1498 Fragments arranged by size: 1779 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3578 5290 96 Size: 274 80 33 51 1443 1601 1712 138 Fragments arranged by size: 1712 1601 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5292 5324 5292 Size: 32 5300 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5332 XbaI T'CTAG_A Cuts at: 2668 2668 Size: 5332 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2482 2482 Size: 5332 XhoI C'TCGA_G Cuts at: 1943 2078 1943 Size: 135 5197 XmaI C'CCGG_G Cuts at: 1870 2657 1870 Size: 787 4545 XmnI GAAnn'nnTTC Cuts at: 3461 3461 Size: 5332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3140 276 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI