Restriction map for
pFN21AE5670
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AspEI AspI AviII
BalI BamHI BbeI BbrPI BbvCI
BciVI BfrI BglII BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssSI
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MflI MluI MluNI MroI
MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1448 GGTACC G^GTACC
AccII 1410 CGCG CG^CG
ApaI 60 GGGCCC GGGCC^C
AscI 1408 GGCGCGCC GG^CGCGCC
AseI 880 ATTAAT AT^TAAT
AsnI 880 ATTAAT AT^TAAT
Asp700I 706 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1448 GGTACC G^GTACC
AvaI 283 CTCGGG C^[CT]CG[GA]G
AvrII 793 CCTAGG C^CTAGG
BanI 1448 GGTACC G^G[CT][GA]CC
BbsI 228 GAAGAC GAAGAC(2/6)
BcgI 467 GCAGCCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1350 CCCGG CC^[GC]GG
BglI 412 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 793 CCTAGG C^CTAGG
BlpI 120 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTGAGC GC^T[ACGT]AGC
BpuAI 228 GAAGAC GAAGAC(2/6)
BsaAI 574 TACGTG [CT]AC^GT[GA]
BsaI 930 GGTCTC GGTCTC(1/5)
BsaWI 1541 ACCGGA [AT]^CCGG[AT]
BseRI 591 CTCCTC GAGGAG(10/8)
BsgI 391 CTGCAC GTGCAG(16/14)
BsmBI 206 GAGACG CGTCTC(1/5)
BsoBI 283 CTCGGG C^[CT]CG[GA]G
Bsp1407I 197 TGTACA T^GTACA
BspMI 1418 ACCTGC ACCTGC(4/8)
BsrGI 197 TGTACA T^GTACA
BssHII 1409 GCGCGC G^CGCGC
BstEII 1084 GGTCACC G^GT[ACGT]ACC
BstPI 1084 GGTCACC G^GT[ACGT]ACC
BstUI 1410 CGCG CG^CG
CelII 120 GCTGAGC GC^T[ACGT]AGC
DraI 1519 TTTAAA TTT^AAA
DraII 734 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 1335 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 734 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1084 GGTCACC G^GT[ACGT]ACC
EcoRI 706 GAATTC G^AATTC
KpnI 1448 GGTACC GGTAC^C
MfeI 962 CAATTG C^AATTG
MunI 962 CAATTG C^AATTG
MvnI 1410 CGCG CG^CG
NciI 1350 CCCGG CC^[GC]GG
NdeI 1143 CATATG CA^TATG
PpuMI 734 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 880 ATTAAT AT^TAAT
PvuII 145 CAGCTG CAG^CTG
SexAI 1444 ACCTGGT A^CC[AT]GGT
SspBI 197 TGTACA T^GTACA
StuI 583 AGGCCT AGG^CCT
TaqI 1027 TCGA T^CGA
TthHB8I 1027 TCGA T^CGA
XmnI 706 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 23 GACGCC G[GA]^CG[CT]C
187 GACGCC
AlwI 96 GATCC GGATC(4/5)
922 GGATC
AspHI 403 GAGCTC G[AT]GC[AT]^C
744 GAGCTC
BclI 326 TGATCA T^GATCA
1185 TGATCA
BfaI 178 CTAG C^TAG
794 CTAG
BsaHI 23 GACGCC G[GA]^CG[CT]C
187 GACGCC
BsiHKAI 403 GAGCTC G[AT]GC[AT]^C
744 GAGCTC
BsrDI 642 CATTGC GCAATG(2/0)
1148 GCAATG
BstAPI 516 GCATGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
691 GCAACCCTTGC
DsaI 1125 CCATGG C^C[GA][CT]GG
1160 CCATGG
EaeI 1370 TGGCCG [CT]^GGCC[GA]
1487 TGGCCG
FauI 1426 CCCGC CCCGC(4/6)
624 GCGGG
FbaI 326 TGATCA T^GATCA
1185 TGATCA
HapII 1351 CCGG C^CGG
1542 CCGG
HgaI 23 GACGC GACGC(5/10)
187 GACGC
Hin1I 23 GACGCC G[GA]^CG[CT]C
187 GACGCC
HpaII 1351 CCGG C^CGG
1542 CCGG
MaeI 178 CTAG C^TAG
794 CTAG
MslI 521 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CATGGTGATG
MspI 1351 CCGG C^CGG
1542 CCGG
NcoI 1125 CCATGG C^CATGG
1160 CCATGG
PflMI 100 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1458 CCATTCCTTGG
SacI 403 GAGCTC GAGCT^C
744 GAGCTC
SphI 125 GCATGC GCATG^C
516 GCATGC
Van91I 100 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1458 CCATTCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI