(Circular) (Six-base) MAPSORT of: pFN21AE5670.seq Check: 2827 from: 1 to: 6304 pFN21AE5670 6304 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3432 3432 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3453 3625 3453 Size: 172 6132 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 3285 4367 5751 6019 1632 Size: 1070 583 1082 1384 268 1917 Fragments arranged by size: 1917 1384 1082 1070 583 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2548 1184 Size: 1364 4940 AlwNI CAG_nnn'CTG Cuts at: 2121 2152 2494 4047 5619 2121 Size: 31 342 1553 1572 2806 Fragments arranged by size: 2806 1572 1553 342 31 ApaI G_GGCC'C Cuts at: 2069 2069 Size: 6304 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 2543 2711 2870 2943 3613 3839 3991 6100 Size: 1050 168 159 73 670 226 152 2109 Cuts at: 6100 1493 Size: 1697 Fragments arranged by size: 2109 1697 1050 670 226 168 159 152 73 AscI GG'CGCG_CC Cuts at: 3414 3414 Size: 6304 AseI AT'TA_AT Cuts at: 160 1032 2886 4858 160 Size: 872 1854 1972 1606 Fragments arranged by size: 1972 1854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2288 3629 1870 Size: 73 345 1341 4545 Fragments arranged by size: 4545 1341 345 73 AvrII C'CTAG_G Cuts at: 2798 2798 Size: 6304 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3453 3625 5080 618 Size: 325 217 627 1666 172 1455 1842 Fragments arranged by size: 1842 1666 1455 627 325 217 172 BanII G_rGCy'C Cuts at: 1391 2069 2412 2753 3623 1391 Size: 678 343 341 870 4072 Fragments arranged by size: 4072 870 678 343 341 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2240 928 Size: 971 341 4992 Fragments arranged by size: 4992 971 341 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2461 2495 4495 4529 1903 Size: 34 524 34 2000 34 3678 Fragments arranged by size: 3678 2000 524 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 2331 3190 1548 Size: 783 859 4662 Fragments arranged by size: 4662 859 783 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 4915 136 Size: 107 122 71 631 605 751 2492 1525 Fragments arranged by size: 2492 1525 751 631 605 122 107 71 BglII A'GATC_T Cuts at: 6299 6299 Size: 6304 BlpI GC'TnA_GC Cuts at: 2126 3721 2126 Size: 1595 4709 Bme1580I G_kGCm'C Cuts at: 1790 2069 2564 2586 4365 5521 1790 Size: 279 495 22 1779 1156 2573 Fragments arranged by size: 2573 1779 1156 495 279 22 BmgBI CAC'GTC Cuts at: 1318 2677 3475 1318 Size: 1359 798 4147 Fragments arranged by size: 4147 1359 798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 2099 2482 Size: 416 53 83 399 24 646 36 383 Cuts at: 2482 2551 4035 4993 442 Size: 69 1484 958 1753 Fragments arranged by size: 1753 1484 958 646 416 399 383 83 69 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2916 3252 3319 4964 1239 Size: 1677 336 67 1645 2579 Fragments arranged by size: 2579 1677 1645 336 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4429 5294 5592 4429 Size: 865 298 5141 Fragments arranged by size: 5141 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2941 4967 882 Size: 617 1442 2026 2219 Fragments arranged by size: 2219 2026 1442 617 BsaAI yAC'GTr Cuts at: 493 1204 2581 4095 493 Size: 711 1377 1514 2702 Fragments arranged by size: 2702 1514 1377 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 2193 4111 4493 Size: 53 83 186 936 496 164 1918 382 Cuts at: 4493 5128 275 Size: 635 1451 Fragments arranged by size: 1918 1451 936 635 496 382 186 164 83 53 BsaWI w'CCGG_w Cuts at: 3546 4737 5135 5409 5556 5967 3546 Size: 1191 398 274 147 411 3883 Fragments arranged by size: 3883 1191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2558 2588 3219 3249 1178 Size: 30 165 30 1155 30 631 30 4233 Fragments arranged by size: 4233 1155 631 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2587 1186 Size: 1401 4903 BseYI C'CCAG_C Cuts at: 2062 2084 5507 6003 2062 Size: 22 3423 496 2363 Fragments arranged by size: 3423 2363 496 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 5086 Fragments arranged by size: 5086 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2412 2753 4365 4450 5521 2412 Size: 341 1612 85 1071 3195 Fragments arranged by size: 3195 1612 1071 341 85 BsmI GAATG_Cn' Cuts at: 3815 3908 3815 Size: 93 6211 BsmBI CGTCTCn'nnnn_ Cuts at: 2205 2205 Size: 6304 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2412 2564 2586 2753 3623 4365 Size: 399 279 343 152 22 167 870 742 Cuts at: 4365 4450 5521 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 870 742 399 343 279 167 152 85 22 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3432 3659 844 Size: 2588 227 3489 Fragments arranged by size: 3489 2588 227 BsrBI CCG'CTC Cuts at: 1394 1823 4191 1394 Size: 429 2368 3507 Fragments arranged by size: 3507 2368 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2646 3160 4799 4973 65 Size: 1183 1398 514 1639 174 1396 Fragments arranged by size: 1639 1398 1396 1183 514 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4948 5135 1700 Size: 110 3138 187 2869 Fragments arranged by size: 3138 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2202 96 Size: 2106 4198 BssHII G'CGCG_C Cuts at: 1925 3414 1925 Size: 1489 4815 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 2527 2702 794 Size: 1733 175 4396 Fragments arranged by size: 4396 1733 175 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 3089 1184 Size: 1905 4399 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6304 BstYI r'GATC_y Cuts at: 1972 3634 4001 4386 4403 5855 5941 5953 6299 Size: 1662 367 385 17 1452 86 12 346 Cuts at: 6299 1972 Size: 1977 Fragments arranged by size: 1977 1662 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3130 3165 513 Size: 552 939 1126 35 3652 Fragments arranged by size: 3652 1126 939 552 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 2525 3820 4614 4634 2525 Size: 1295 794 20 4195 Fragments arranged by size: 4195 1295 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3526 3608 3526 Size: 82 6222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3375 3492 3751 Size: 54 1425 50 275 18 1545 117 259 Cuts at: 3751 4640 6269 8 Size: 889 1629 43 Fragments arranged by size: 1629 1545 1425 889 275 259 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4234 1353 Size: 2881 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 4891 5265 5411 387 Size: 1064 138 451 2851 374 146 1280 Fragments arranged by size: 2851 1280 1064 451 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 2751 2410 Size: 341 5963 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2702 2916 3252 3285 3319 4367 4964 Size: 393 1070 214 336 33 34 1048 597 Cuts at: 4964 5751 6019 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1070 1048 787 597 393 336 268 214 34 33 EcoNI CCTnn'n_nnAGG Cuts at: 3344 3344 Size: 6304 EcoO109I rG'GnC_Cy Cuts at: 2740 2740 Size: 6304 EcoRI G'AATT_C Cuts at: 2711 3613 3991 2711 Size: 902 378 5024 Fragments arranged by size: 5024 902 378 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2919 2951 4945 4977 5019 Size: 32 135 32 1347 32 1994 32 42 Cuts at: 5019 5051 1373 Size: 32 2626 Fragments arranged by size: 2626 1994 1347 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3648 3894 669 Size: 988 281 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3457 3629 3457 Size: 172 6132 MfeI C'AATT_G Cuts at: 2967 3903 2967 Size: 936 5368 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5418 5602 1305 Size: 4113 184 2007 Fragments arranged by size: 4113 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4482 Fragments arranged by size: 4482 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2530 3170 4262 4621 4780 518 Size: 648 843 521 640 1092 359 159 2042 Fragments arranged by size: 2042 1092 843 648 640 521 359 159 MspA1I CmG'CkG Cuts at: 1606 2152 2629 3367 3720 4397 5545 5790 1606 Size: 546 477 738 353 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 546 477 353 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3130 3165 513 Size: 552 939 1126 35 3652 Fragments arranged by size: 3652 1126 939 552 35 NdeI CA'TA_TG Cuts at: 387 3149 387 Size: 2762 3542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 2134 2525 2644 3662 5207 1132 Size: 97 905 391 119 1018 1545 2229 Fragments arranged by size: 2229 1545 1018 905 391 119 97 PasI CC'CwG_GG Cuts at: 1348 2020 1348 Size: 672 5632 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 2111 3469 1108 Size: 1003 1358 3943 Fragments arranged by size: 3943 1358 1003 PfoI T'CCnGG_A Cuts at: 2736 2736 Size: 6304 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2740 2740 Size: 6304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspOMI G'GGCC_C Cuts at: 2065 2065 Size: 6304 PstI C_TGCA'G Cuts at: 830 2157 2325 2628 3656 830 Size: 1327 168 303 1028 3478 Fragments arranged by size: 3478 1327 1028 303 168 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2152 2152 Size: 6304 SacI G_AGCT'C Cuts at: 2412 2753 2412 Size: 341 5963 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SexAI A'CCwGG_T Cuts at: 3449 3449 Size: 6304 SfcI C'TryA_G Cuts at: 826 1045 2153 2321 2624 3652 4787 5468 5659 Size: 219 1108 168 303 1028 1135 681 191 Cuts at: 5659 826 Size: 1471 Fragments arranged by size: 1471 1135 1108 1028 681 303 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4408 5309 5571 820 Size: 197 926 2465 901 262 1553 Fragments arranged by size: 2465 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 2134 2525 3662 1132 Size: 1002 391 1137 3774 Fragments arranged by size: 3774 1137 1002 391 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 2590 1517 Size: 1073 5231 StyI C'CwwG_G Cuts at: 513 1065 2004 2798 3130 3165 3468 3743 513 Size: 552 939 794 332 35 303 275 3074 Fragments arranged by size: 3074 939 794 552 332 303 275 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3131 3376 4331 4348 4501 4686 5131 Size: 1255 1551 245 955 17 153 185 445 Cuts at: 5131 325 Size: 1498 Fragments arranged by size: 1551 1498 1255 955 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 4550 6262 Size: 274 80 33 51 1443 225 2348 1712 Cuts at: 6262 96 Size: 138 Fragments arranged by size: 2348 1712 1443 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3219 3251 6264 6296 3219 Size: 32 3013 32 3227 Fragments arranged by size: 3227 3013 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 2715 4433 2715 Size: 1718 4586 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI