(Linear) (Six-base) MAPSORT of: KIEE5670.seq Check: 9570 from: 1 to: 1599 KIEE5670.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1427 1599 Size: 1427 172 Acc65I G'GTAC_C Cuts at: 0 1448 1599 Size: 1448 151 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 697 1280 1599 Size: 697 583 319 Fragments arranged by size: 697 583 319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 543 1599 Size: 543 1056 AlwNI CAG_nnn'CTG Cuts at: 0 116 147 489 1599 Size: 116 31 342 1110 Fragments arranged by size: 1110 342 116 31 ApaI G_GGCC'C Cuts at: 0 64 1599 Size: 64 1535 ApoI r'AATT_y Cuts at: 0 538 706 865 938 1599 Size: 538 168 159 73 661 Fragments arranged by size: 661 538 168 159 73 AscI GG'CGCG_CC Cuts at: 0 1409 1599 Size: 1409 190 AseI AT'TA_AT Cuts at: 0 881 1599 Size: 881 718 AvaI C'yCGr_G Cuts at: 0 283 1599 Size: 283 1316 AvrII C'CTAG_G Cuts at: 0 793 1599 Size: 793 806 BanI G'GyrC_C Cuts at: 0 1448 1599 Size: 1448 151 BanII G_rGCy'C Cuts at: 0 64 407 748 1599 Size: 64 343 341 851 Fragments arranged by size: 851 343 341 64 BbsI GAAGACnn'nnnn_ Cuts at: 0 235 1599 Size: 235 1364 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 456 490 1599 Size: 456 34 1109 Fragments arranged by size: 1109 456 34 BclI T'GATC_A Cuts at: 0 326 1185 1599 Size: 326 859 414 Fragments arranged by size: 859 414 326 BglI GCCn_nnn'nGGC Cuts at: 0 418 1599 Size: 418 1181 BlpI GC'TnA_GC Cuts at: 0 121 1599 Size: 121 1478 Bme1580I G_kGCm'C Cuts at: 0 64 559 581 1599 Size: 64 495 22 1018 Fragments arranged by size: 1018 495 64 22 BmgBI CAC'GTC Cuts at: 0 672 1470 1599 Size: 672 798 129 Fragments arranged by size: 798 672 129 BmrI ACTGGGnnnn_n' Cuts at: 0 58 94 477 546 1599 Size: 58 36 383 69 1053 Fragments arranged by size: 1053 383 69 58 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 911 1247 1314 1599 Size: 911 336 67 285 Fragments arranged by size: 911 336 285 67 BsaI GGTCTCn'nnnn_ Cuts at: 0 936 1599 Size: 936 663 BsaAI yAC'GTr Cuts at: 0 576 1599 Size: 576 1023 BsaHI Gr'CG_yC Cuts at: 0 24 188 1599 Size: 24 164 1411 Fragments arranged by size: 1411 164 24 BsaWI w'CCGG_w Cuts at: 0 1541 1599 Size: 1541 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 553 583 1214 1244 1599 Size: 553 30 631 30 355 Fragments arranged by size: 631 553 355 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 582 1599 Size: 582 1017 BseYI C'CCAG_C Cuts at: 0 57 79 1599 Size: 57 22 1520 Fragments arranged by size: 1520 57 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 1599 Size: 376 1223 BsiHKAI G_wGCw'C Cuts at: 0 407 748 1599 Size: 407 341 851 Fragments arranged by size: 851 407 341 BsmBI CGTCTCn'nnnn_ Cuts at: 0 200 1599 Size: 200 1399 Bsp1286I G_dGCh'C Cuts at: 0 64 407 559 581 748 1599 Size: 64 343 152 22 167 851 Fragments arranged by size: 851 343 167 152 64 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1427 1599 Size: 1427 172 BsrDI GCAATG_nn' Cuts at: 0 641 1155 1599 Size: 641 514 444 Fragments arranged by size: 641 514 444 BsrGI T'GTAC_A Cuts at: 0 197 1599 Size: 197 1402 BssHII G'CGCG_C Cuts at: 0 1409 1599 Size: 1409 190 BstAPI GCAn_nnn'nTGC Cuts at: 0 522 697 1599 Size: 522 175 902 Fragments arranged by size: 902 522 175 BstEII G'GTnAC_C Cuts at: 0 1084 1599 Size: 1084 515 BtgI C'CryG_G Cuts at: 0 1125 1160 1599 Size: 1125 35 439 Fragments arranged by size: 1125 439 35 BtsI GCAGTG_nn' Cuts at: 0 520 1599 Size: 520 1079 DraI TTT'AAA Cuts at: 0 1521 1599 Size: 1521 78 EaeI y'GGCC_r Cuts at: 0 1370 1487 1599 Size: 1370 117 112 Fragments arranged by size: 1370 117 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 35 1599 Size: 35 1564 EcoICRI GAG'CTC Cuts at: 0 405 746 1599 Size: 405 341 853 Fragments arranged by size: 853 405 341 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 697 911 1247 1280 1314 1599 Size: 697 214 336 33 34 285 Fragments arranged by size: 697 336 285 214 34 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 1339 1599 Size: 1339 260 EcoO109I rG'GnC_Cy Cuts at: 0 735 1599 Size: 735 864 EcoRI G'AATT_C Cuts at: 0 706 1599 Size: 706 893 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 914 946 1599 Size: 914 32 653 Fragments arranged by size: 914 653 32 KpnI G_GTAC'C Cuts at: 0 1452 1599 Size: 1452 147 MfeI C'AATT_G Cuts at: 0 962 1599 Size: 962 637 MslI CAynn'nnrTG Cuts at: 0 525 1165 1599 Size: 525 640 434 Fragments arranged by size: 640 525 434 MspA1I CmG'CkG Cuts at: 0 147 624 1362 1599 Size: 147 477 738 237 Fragments arranged by size: 738 477 237 147 NcoI C'CATG_G Cuts at: 0 1125 1160 1599 Size: 1125 35 439 Fragments arranged by size: 1125 439 35 NdeI CA'TA_TG Cuts at: 0 1144 1599 Size: 1144 455 NspI r_CATG'y Cuts at: 0 129 520 639 1599 Size: 129 391 119 960 Fragments arranged by size: 960 391 129 119 PasI CC'CwG_GG Cuts at: 0 15 1599 Size: 15 1584 PflMI CCAn_nnn'nTGG Cuts at: 0 106 1464 1599 Size: 106 1358 135 Fragments arranged by size: 1358 135 106 PfoI T'CCnGG_A Cuts at: 0 731 1599 Size: 731 868 PpuMI rG'GwC_Cy Cuts at: 0 735 1599 Size: 735 864 PspOMI G'GGCC_C Cuts at: 0 60 1599 Size: 60 1539 PstI C_TGCA'G Cuts at: 0 152 320 623 1599 Size: 152 168 303 976 Fragments arranged by size: 976 303 168 152 PvuII CAG'CTG Cuts at: 0 147 1599 Size: 147 1452 SacI G_AGCT'C Cuts at: 0 407 748 1599 Size: 407 341 851 Fragments arranged by size: 851 407 341 SexAI A'CCwGG_T Cuts at: 0 1444 1599 Size: 1444 155 SfcI C'TryA_G Cuts at: 0 148 316 619 1599 Size: 148 168 303 980 Fragments arranged by size: 980 303 168 148 SphI G_CATG'C Cuts at: 0 129 520 1599 Size: 129 391 1079 Fragments arranged by size: 1079 391 129 StuI AGG'CCT Cuts at: 0 585 1599 Size: 585 1014 StyI C'CwwG_G Cuts at: 0 793 1125 1160 1463 1599 Size: 793 332 35 303 136 Fragments arranged by size: 793 332 303 136 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1126 1371 1599 Size: 1126 245 228 Fragments arranged by size: 1126 245 228 TatI w'GTAC_w Cuts at: 0 197 1599 Size: 197 1402 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1214 1246 1599 Size: 1214 32 353 Fragments arranged by size: 1214 353 32 XmnI GAAnn'nnTTC Cuts at: 0 710 1599 Size: 710 889 Enzymes that do cut: AarI Acc65I AcuI AlfI AlwNI ApaI ApoI AscI AseI AvaI AvrII BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BssHII BstAPI BstEII BtgI BtsI DraI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I KpnI MfeI MslI MspA1I NcoI NdeI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SexAI SfcI SphI StuI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AsiSI BaeI BamHI BbeI BbvCI BciVI BfrBI BglII BmtI BplI Bpu10I BpuEI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EarI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MluI MmeI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI