(Circular) (Six-base) MAPSORT of: pFN21AE5665.seq Check: 7921 from: 1 to: 6289 pFN21AE5665 6289 bp With 182 enzymes: SgfI * October 12, 2018 18:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3632 1937 Size: 1695 4594 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4352 5736 6004 1632 Size: 2720 1384 268 1917 Fragments arranged by size: 2720 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 2679 3225 3258 5188 6165 1203 Size: 1476 546 33 1930 977 1327 Fragments arranged by size: 1930 1476 1327 977 546 33 AgeI A'CCGG_T Cuts at: 5120 5120 Size: 6289 AhdI GACnn_n'nnGTC Cuts at: 5018 5018 Size: 6289 AleI CACnn'nnGTG Cuts at: 1166 2510 2787 1166 Size: 1344 277 4668 Fragments arranged by size: 4668 1344 277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2805 3543 1184 Size: 1621 738 3930 Fragments arranged by size: 3930 1621 738 AlwNI CAG_nnn'CTG Cuts at: 2576 2705 2942 4032 5604 2576 Size: 129 237 1090 1572 3261 Fragments arranged by size: 3261 1572 1090 237 129 ApaI G_GGCC'C Cuts at: 2016 2016 Size: 6289 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 3250 3614 1870 Size: 73 1307 364 4545 Fragments arranged by size: 4545 1307 364 73 BamHI G'GATC_C Cuts at: 2220 3619 3986 2220 Size: 1399 367 4523 Fragments arranged by size: 4523 1399 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 2326 2534 2607 2789 Size: 325 217 627 410 129 208 73 182 Cuts at: 2789 3334 3490 3610 5065 618 Size: 545 156 120 1455 1842 Fragments arranged by size: 1842 1455 627 545 410 325 217 208 182 156 129 120 73 BanII G_rGCy'C Cuts at: 1391 2016 2820 2874 3179 3311 3471 3608 1391 Size: 625 804 54 305 132 160 137 4072 Fragments arranged by size: 4072 804 625 305 160 137 132 54 BbeI G_GCGC'C Cuts at: 3494 3494 Size: 6289 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3136 3372 928 Size: 971 1237 236 3845 Fragments arranged by size: 3845 1237 971 236 BbvCI CC'TCA_GC Cuts at: 2073 2112 2590 2073 Size: 39 478 5772 Fragments arranged by size: 5772 478 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4480 4514 1903 Size: 34 50 34 2459 34 3678 Fragments arranged by size: 3678 2459 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4900 136 Size: 107 122 71 631 605 3228 1525 Fragments arranged by size: 3228 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6284 6284 Size: 6289 BlpI GC'TnA_GC Cuts at: 2957 3706 2957 Size: 749 5540 Bme1580I G_kGCm'C Cuts at: 1790 2016 2202 3339 4350 5506 1790 Size: 226 186 1137 1011 1156 2573 Fragments arranged by size: 2573 1156 1137 1011 226 186 BmgBI CAC'GTC Cuts at: 1318 2653 3228 3286 3370 1318 Size: 1335 575 58 84 4237 Fragments arranged by size: 4237 1335 575 84 58 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2258 3518 4020 Size: 416 53 83 399 24 841 1260 502 Cuts at: 4020 4978 442 Size: 958 1753 Fragments arranged by size: 1753 1260 958 841 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3485 4949 1239 Size: 2246 1464 2579 Fragments arranged by size: 2579 2246 1464 Bpu10I CC'TnA_GC Cuts at: 2073 2112 2590 3207 2073 Size: 39 478 617 5155 Fragments arranged by size: 5155 617 478 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2416 4414 5279 5577 2416 Size: 1998 865 298 3128 Fragments arranged by size: 3128 1998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2895 3344 4952 882 Size: 617 1396 449 1608 2219 Fragments arranged by size: 2219 1608 1396 617 449 BsaAI yAC'GTr Cuts at: 493 1204 3259 4080 493 Size: 711 2055 821 2702 Fragments arranged by size: 2702 2055 821 711 BsaBI GATnn'nnATC Cuts at: 6283 6283 Size: 6289 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2261 2828 3069 Size: 53 83 186 936 528 200 567 241 Cuts at: 3069 3491 3513 3575 4096 4478 5113 275 Size: 422 22 62 521 382 635 1451 Fragments arranged by size: 1451 936 635 567 528 521 422 382 241 200 186 83 62 53 22 BsaWI w'CCGG_w Cuts at: 4722 5120 5394 5541 5952 4722 Size: 398 274 147 411 5059 Fragments arranged by size: 5059 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6064 Fragments arranged by size: 6064 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2093 3116 1186 Size: 904 3 1023 4359 Fragments arranged by size: 4359 1023 904 3 BseYI C'CCAG_C Cuts at: 2032 2068 2713 3197 3568 5492 5988 2032 Size: 36 645 484 371 1924 496 2333 Fragments arranged by size: 2333 1924 645 496 484 371 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2421 2534 2783 3437 3487 1163 Size: 573 685 113 249 654 50 3965 Fragments arranged by size: 3965 685 654 573 249 113 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3739 4500 5528 1540 Size: 275 186 1000 738 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 738 275 186 BsiHKAI G_wGCw'C Cuts at: 2820 3179 4350 4435 5506 2820 Size: 359 1171 85 1071 3603 Fragments arranged by size: 3603 1171 1071 359 85 BsmI GAATG_Cn' Cuts at: 3800 3893 3800 Size: 93 6196 BsmBI CGTCTCn'nnnn_ Cuts at: 2252 2252 Size: 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2202 2820 2874 3179 3311 3339 Size: 399 226 186 618 54 305 132 28 Cuts at: 3339 3471 3608 4350 4435 5506 1391 Size: 132 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 618 399 305 226 186 137 132 132 85 54 28 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 3019 4178 5908 3019 Size: 1159 1730 3400 Fragments arranged by size: 3400 1730 1159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3644 844 Size: 2800 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3303 4176 1394 Size: 429 1480 873 3507 Fragments arranged by size: 3507 1480 873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2446 3250 4784 4958 65 Size: 1183 1198 804 1534 174 1396 Fragments arranged by size: 1534 1396 1198 1183 804 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2531 4933 5120 1700 Size: 110 721 2402 187 2869 Fragments arranged by size: 2869 2402 721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 3383 1925 Size: 1458 4831 BssSI C'ACGA_G Cuts at: 4349 5361 4349 Size: 1012 5277 BstAPI GCAn_nnn'nTGC Cuts at: 794 3406 3415 794 Size: 2612 9 3668 Fragments arranged by size: 3668 2612 9 BstBI TT'CG_AA Cuts at: 5093 5093 Size: 6289 BstEII G'GTnAC_C Cuts at: 1184 2497 1184 Size: 1313 4976 BstYI r'GATC_y Cuts at: 1972 2220 2892 3619 3986 4371 4388 5840 5926 Size: 248 672 727 367 385 17 1452 86 Cuts at: 5926 5938 6284 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 727 672 385 367 346 248 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2039 2039 Size: 6289 BtgI C'CryG_G Cuts at: 513 1065 2202 2271 2349 2688 2971 3010 3355 Size: 552 1137 69 78 339 283 39 345 Cuts at: 3355 513 Size: 3447 Fragments arranged by size: 3447 1137 552 345 339 283 78 69 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2199 2602 487 Size: 683 56 973 403 4174 Fragments arranged by size: 4174 973 683 403 56 BtsI GCAGTG_nn' Cuts at: 3542 3805 4599 4619 3542 Size: 263 794 20 5212 Fragments arranged by size: 5212 794 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6254 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DraIII CAC_nnn'GTG Cuts at: 2551 3406 2551 Size: 855 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5296 809 Size: 507 3980 1802 Fragments arranged by size: 3980 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 2998 3549 Size: 54 1425 50 275 18 438 730 551 Cuts at: 3549 3736 4625 6254 8 Size: 187 889 1629 43 Fragments arranged by size: 1629 1425 889 730 551 438 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 3736 1537 Size: 275 1186 738 4090 Fragments arranged by size: 4090 1186 738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2180 4219 1353 Size: 827 2039 3423 Fragments arranged by size: 3423 2039 827 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2679 4876 5250 5396 387 Size: 1064 138 1090 2197 374 146 1280 Fragments arranged by size: 2197 1280 1090 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2818 3177 2818 Size: 359 5930 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3485 4352 4949 5736 6004 1239 Size: 393 1853 867 597 787 268 1524 Fragments arranged by size: 1853 1524 867 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2762 2762 Size: 6289 EcoO109I rG'GnC_Cy Cuts at: 2012 2013 2116 2252 2694 3555 2012 Size: 1 103 136 442 861 4746 Fragments arranged by size: 4746 861 442 136 103 1 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 2395 Size: 6289 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 3494 5436 1678 Size: 1816 1942 2531 Fragments arranged by size: 2531 1942 1816 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373 Size: 32 135 32 3358 32 42 32 2626 Fragments arranged by size: 3358 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3633 3879 669 Size: 988 281 1695 246 3079 Fragments arranged by size: 3079 1695 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KasI G'GCGC_C Cuts at: 3490 3490 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2661 5403 5587 1305 Size: 1356 2742 184 2007 Fragments arranged by size: 2742 2007 1356 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 4029 Fragments arranged by size: 4029 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 2510 2651 2787 3024 3138 4247 Size: 648 1287 57 141 136 237 114 1109 Cuts at: 4247 4606 4765 518 Size: 359 159 2042 Fragments arranged by size: 2042 1287 1109 648 359 237 159 141 136 114 57 MspA1I CmG'CkG Cuts at: 1606 2072 2111 2717 2973 3128 3581 3705 4382 Size: 466 39 606 256 155 453 124 677 Cuts at: 4382 5530 5775 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 606 466 453 256 245 155 124 39 NaeI GCC'GGC Cuts at: 2533 2533 Size: 6289 NarI GG'CG_CC Cuts at: 3491 3491 Size: 6289 NcoI C'CATG_G Cuts at: 513 1065 2271 2688 3010 513 Size: 552 1206 417 322 3792 Fragments arranged by size: 3792 1206 552 417 322 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NgoMIV G'CCGG_C Cuts at: 2531 2531 Size: 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 2998 3736 2998 Size: 738 5551 NspI r_CATG'y Cuts at: 1132 1229 2584 2683 3443 3647 5192 1132 Size: 97 1355 99 760 204 1545 2229 Fragments arranged by size: 2229 1545 1355 760 204 99 97 PasI CC'CwG_GG Cuts at: 1348 2016 2056 1348 Size: 668 40 5581 Fragments arranged by size: 5581 668 40 PciI A'CATG_T Cuts at: 2679 5188 2679 Size: 2509 3780 PflMI CCAn_nnn'nTGG Cuts at: 1108 2857 1108 Size: 1749 4540 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2694 3555 2694 Size: 861 5428 PshAI GACnn'nnGTC Cuts at: 1613 3012 3119 1613 Size: 1399 107 4783 Fragments arranged by size: 4783 1399 107 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 2012 2012 Size: 6289 PstI C_TGCA'G Cuts at: 830 2142 3641 830 Size: 1312 1499 3478 Fragments arranged by size: 3478 1499 1312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 2072 2111 2717 2072 Size: 39 606 5644 Fragments arranged by size: 5644 606 39 SacI G_AGCT'C Cuts at: 2820 3179 2820 Size: 359 5930 SacII CC_GC'GG Cuts at: 2974 2974 Size: 6289 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SanDI GG'GwC_CC Cuts at: 2694 3555 2694 Size: 861 5428 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SexAI A'CCwGG_T Cuts at: 2757 3402 2757 Size: 645 5644 SfcI C'TryA_G Cuts at: 826 1045 2045 2138 3637 4772 5453 5644 826 Size: 219 1000 93 1499 1135 681 191 1471 Fragments arranged by size: 1499 1471 1135 1000 681 219 191 93 SfoI GGC'GCC Cuts at: 3492 3492 Size: 6289 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3252 3616 1872 Size: 1380 364 4545 Fragments arranged by size: 4545 1380 364 SmlI C'TyrA_G Cuts at: 820 1017 1943 2395 4393 5294 5556 820 Size: 197 926 452 1998 901 262 1553 Fragments arranged by size: 1998 1553 926 901 452 262 197 SnaBI TAC'GTA Cuts at: 493 4080 493 Size: 3587 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3647 1132 Size: 2515 3774 SrfI GCCC'GGGC Cuts at: 3252 3252 Size: 6289 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6289 StyI C'CwwG_G Cuts at: 513 1065 2271 2659 2688 3010 3728 513 Size: 552 1206 388 29 322 718 3074 Fragments arranged by size: 3074 1206 718 552 388 322 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 3537 4316 4333 4486 4671 5116 Size: 1255 428 1529 779 17 153 185 445 Cuts at: 5116 325 Size: 1498 Fragments arranged by size: 1529 1498 1255 779 445 428 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2676 4535 6247 Size: 274 80 33 51 1443 699 1859 1712 Cuts at: 6247 96 Size: 138 Fragments arranged by size: 1859 1712 1443 699 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6249 6281 6249 Size: 32 6257 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6289 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3187 3187 Size: 6289 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 3250 3614 1870 Size: 1380 364 4545 Fragments arranged by size: 4545 1380 364 XmnI GAAnn'nnTTC Cuts at: 4418 4418 Size: 6289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SfiI SwaI