(Circular) (Six-base) MAPSORT of: pFN21AB8519.seq Check: 573 from: 1 to: 7564 pFN21AB8519 7564 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4211 4211 Size: 7564 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2490 5374 278 Size: 53 83 186 936 954 2884 2468 Fragments arranged by size: 2884 2468 954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2130 3755 4907 1937 Size: 193 1625 1152 4594 Fragments arranged by size: 4594 1625 1152 193 Acc65I G'GTAC_C Cuts at: 4885 4885 Size: 7564 AclI AA'CG_TT Cuts at: 1563 5691 6064 1563 Size: 4128 373 3063 Fragments arranged by size: 4128 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 4059 4766 5627 7011 7279 1632 Size: 750 1677 707 861 1384 268 1917 Fragments arranged by size: 1917 1677 1384 861 750 707 268 AflII C'TTAA_G Cuts at: 820 1017 2060 2546 3398 4670 820 Size: 197 1043 486 852 1272 3714 Fragments arranged by size: 3714 1272 1043 852 486 197 AflIII A'CryG_T Cuts at: 1203 3814 6463 7440 1203 Size: 2611 2649 977 1327 Fragments arranged by size: 2649 2611 1327 977 AgeI A'CCGG_T Cuts at: 6395 6395 Size: 7564 AhdI GACnn_n'nnGTC Cuts at: 2486 6293 2486 Size: 3807 3757 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7564 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7564 AlwNI CAG_nnn'CTG Cuts at: 5307 6879 5307 Size: 1572 5992 ApaLI G'TGCA_C Cuts at: 5621 6777 5621 Size: 1156 6408 ApoI r'AATT_y Cuts at: 1493 2066 2115 2985 3088 3206 4873 5099 5251 Size: 573 49 870 103 118 1667 226 152 Cuts at: 5251 7360 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1667 870 573 226 152 118 103 49 AseI AT'TA_AT Cuts at: 160 1032 4011 6118 160 Size: 872 2979 2107 1606 Fragments arranged by size: 2979 2107 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7564 AvaI C'yCGr_G Cuts at: 1870 1943 4650 4889 1870 Size: 73 2707 239 4545 Fragments arranged by size: 4545 2707 239 73 AvrII C'CTAG_G Cuts at: 3550 3550 Size: 7564 BamHI G'GATC_C Cuts at: 2843 4894 5261 2843 Size: 2051 367 5146 Fragments arranged by size: 5146 2051 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 4885 6340 618 Size: 325 217 627 1032 2066 1455 1842 Fragments arranged by size: 2066 1842 1455 1032 627 325 217 BanII G_rGCy'C Cuts at: 1391 3572 4883 1391 Size: 2181 1311 4072 Fragments arranged by size: 4072 2181 1311 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2485 2727 4767 928 Size: 971 586 242 2040 3725 Fragments arranged by size: 3725 2040 971 586 242 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3400 3434 5755 5789 1903 Size: 34 1463 34 2321 34 3678 Fragments arranged by size: 3678 2321 1463 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3286 4744 5456 6666 3286 Size: 1458 712 1210 4184 Fragments arranged by size: 4184 1458 1210 712 BclI T'GATC_A Cuts at: 1548 2007 2320 2434 1548 Size: 459 313 114 6678 Fragments arranged by size: 6678 459 313 114 BfrBI ATG'CAT Cuts at: 2316 2316 Size: 7564 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 6175 136 Size: 107 122 71 631 605 520 3983 1525 Fragments arranged by size: 3983 1525 631 605 520 122 107 71 BglII A'GATC_T Cuts at: 7559 7559 Size: 7564 BlpI GC'TnA_GC Cuts at: 4981 4981 Size: 7564 Bme1580I G_kGCm'C Cuts at: 1790 3905 4066 5625 6781 1790 Size: 2115 161 1559 1156 2573 Fragments arranged by size: 2573 2115 1559 1156 161 BmgBI CAC'GTC Cuts at: 1318 3473 1318 Size: 2155 5409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3208 4269 4300 Size: 416 53 83 399 24 1791 1061 31 Cuts at: 4300 5295 6253 442 Size: 995 958 1753 Fragments arranged by size: 1791 1753 1061 995 958 416 399 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 2775 1056 Size: 1719 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 2528 3285 6224 1239 Size: 1250 39 757 2939 2579 Fragments arranged by size: 2939 2579 1250 757 39 Bpu10I CC'TnA_GC Cuts at: 3582 4617 3582 Size: 1035 6529 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5689 6554 6852 5689 Size: 865 298 6401 Fragments arranged by size: 6401 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3335 4685 6227 882 Size: 617 1836 1350 1542 2219 Fragments arranged by size: 2219 1836 1542 1350 617 BsaAI yAC'GTr Cuts at: 493 1204 4051 5355 493 Size: 711 2847 1304 2702 Fragments arranged by size: 2847 2702 1304 711 BsaBI GATnn'nnATC Cuts at: 7558 7558 Size: 7564 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2487 3805 4290 5371 Size: 53 83 186 936 954 1318 485 1081 Cuts at: 5371 5753 6388 275 Size: 382 635 1451 Fragments arranged by size: 1451 1318 1081 954 936 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2166 5997 6395 6669 6816 7227 2166 Size: 3831 398 274 147 411 2503 Fragments arranged by size: 3831 2503 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7339 Fragments arranged by size: 7339 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 4568 4571 4784 4826 4829 1186 Size: 1057 2325 3 213 42 3 3921 Fragments arranged by size: 3921 2325 1057 213 42 3 3 BseYI C'CCAG_C Cuts at: 4645 4665 6767 7263 4645 Size: 20 2102 496 4946 Fragments arranged by size: 4946 2102 496 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3453 1163 Size: 573 1717 5274 Fragments arranged by size: 5274 1717 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 5014 5775 6803 1540 Size: 275 186 18 2995 761 1028 2301 Fragments arranged by size: 2995 2301 1028 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 5625 5710 6781 5625 Size: 85 1071 6408 Fragments arranged by size: 6408 1071 85 BsmI GAATG_Cn' Cuts at: 2092 5075 5168 2092 Size: 2983 93 4488 Fragments arranged by size: 4488 2983 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3572 3905 4066 4883 5625 5710 6781 Size: 399 1782 333 161 817 742 85 1071 Cuts at: 6781 1391 Size: 2174 Fragments arranged by size: 2174 1782 1071 817 742 399 333 161 85 BspEI T'CCGG_A Cuts at: 2166 7227 2166 Size: 5061 2503 BspHI T'CATG_A Cuts at: 3054 3247 4086 5453 7183 3054 Size: 193 839 1367 1730 3435 Fragments arranged by size: 3435 1730 1367 839 193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4211 4919 844 Size: 3367 708 3489 Fragments arranged by size: 3489 3367 708 BsrBI CCG'CTC Cuts at: 1394 1823 5451 1394 Size: 429 3628 3507 Fragments arranged by size: 3628 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 3418 3969 6059 6233 65 Size: 1183 1212 958 551 2090 174 1396 Fragments arranged by size: 2090 1396 1212 1183 958 551 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6208 6395 1700 Size: 110 4398 187 2869 Fragments arranged by size: 4398 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7564 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7564 BssSI C'ACGA_G Cuts at: 5624 6636 5624 Size: 1012 6552 BstAPI GCAn_nnn'nTGC Cuts at: 794 4445 794 Size: 3651 3913 BstBI TT'CG_AA Cuts at: 2809 3739 6368 2809 Size: 930 2629 4005 Fragments arranged by size: 4005 2629 930 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7564 BstYI r'GATC_y Cuts at: 1972 2843 4894 5261 5646 5663 7115 7201 7213 Size: 871 2051 367 385 17 1452 86 12 Cuts at: 7213 7559 1972 Size: 346 1977 Fragments arranged by size: 2051 1977 1452 871 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3756 3756 Size: 7564 Bsu36I CC'TnA_GG Cuts at: 2682 2958 2682 Size: 276 7288 BtgI C'CryG_G Cuts at: 513 1065 3459 3765 513 Size: 552 2394 306 4312 Fragments arranged by size: 4312 2394 552 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2437 487 Size: 683 56 1211 5614 Fragments arranged by size: 5614 1211 683 56 BtsI GCAGTG_nn' Cuts at: 2923 5080 5874 5894 2923 Size: 2157 794 20 4593 Fragments arranged by size: 4593 2157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7564 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3346 3381 529 Size: 35 2782 35 4712 Fragments arranged by size: 4712 2782 35 35 DraI TTT'AAA Cuts at: 4868 4868 Size: 7564 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6571 809 Size: 507 5255 1802 Fragments arranged by size: 5255 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 4179 5011 Size: 54 1425 50 275 18 186 2163 832 Cuts at: 5011 5900 7529 8 Size: 889 1629 43 Fragments arranged by size: 2163 1629 1425 889 832 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 5011 1537 Size: 275 204 2995 4090 Fragments arranged by size: 4090 2995 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 2270 3681 5494 1353 Size: 687 230 1411 1813 3423 Fragments arranged by size: 3423 1813 1411 687 230 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6151 6525 6671 387 Size: 1064 138 4562 374 146 1280 Fragments arranged by size: 4562 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 2489 2528 3285 4059 4766 5627 Size: 393 750 107 39 757 774 707 861 Cuts at: 5627 6224 7011 7279 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 861 787 774 757 750 707 597 393 268 107 39 EcoNI CCTnn'n_nnAGG Cuts at: 3287 3509 4379 3287 Size: 222 870 6472 Fragments arranged by size: 6472 870 222 EcoO109I rG'GnC_Cy Cuts at: 2260 3727 4675 2260 Size: 1467 948 5149 Fragments arranged by size: 5149 1467 948 EcoRI G'AATT_C Cuts at: 3088 4873 5251 3088 Size: 1785 378 5401 Fragments arranged by size: 5401 1785 378 EcoRV GAT'ATC Cuts at: 2698 3418 2698 Size: 720 6844 FspI TGC'GCA Cuts at: 6070 6070 Size: 7564 HaeII r_GCGC'y Cuts at: 1678 4194 6711 1678 Size: 2516 2517 2531 Fragments arranged by size: 2531 2517 2516 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2472 2504 2725 2757 2873 Size: 32 135 32 900 32 221 32 116 Cuts at: 2873 2905 4722 4754 6205 6237 6279 6311 1373 Size: 32 1817 32 1451 32 42 32 2626 Fragments arranged by size: 2626 1817 1451 900 221 135 116 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4908 5154 669 Size: 988 281 2970 246 3079 Fragments arranged by size: 3079 2970 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7564 HpaI GTT'AAC Cuts at: 5154 5154 Size: 7564 KpnI G_GTAC'C Cuts at: 4889 4889 Size: 7564 MfeI C'AATT_G Cuts at: 2379 5163 2379 Size: 2784 4780 MluI A'CGCG_T Cuts at: 7440 7440 Size: 7564 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6678 6862 1305 Size: 5373 184 2007 Fragments arranged by size: 5373 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4181 10 Size: 54 1425 343 2349 3393 Fragments arranged by size: 3393 2349 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3366 4170 5522 5881 6040 518 Size: 648 2200 804 1352 359 159 2042 Fragments arranged by size: 2200 2042 1352 804 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2021 3539 4138 4980 5657 6805 7050 1606 Size: 415 1518 599 842 677 1148 245 2120 Fragments arranged by size: 2120 1518 1148 842 677 599 415 245 NcoI C'CATG_G Cuts at: 513 1065 3459 3765 513 Size: 552 2394 306 4312 Fragments arranged by size: 4312 2394 552 306 NdeI CA'TA_TG Cuts at: 387 4272 387 Size: 3885 3679 NheI G'CTAG_C Cuts at: 1052 2771 1052 Size: 1719 5845 NotI GC'GGCC_GC Cuts at: 5011 5011 Size: 7564 NsiI A_TGCA'T Cuts at: 2318 2318 Size: 7564 NspI r_CATG'y Cuts at: 1132 1229 2560 3818 4922 6467 1132 Size: 97 1331 1258 1104 1545 2229 Fragments arranged by size: 2229 1545 1331 1258 1104 97 PasI CC'CwG_GG Cuts at: 1348 4204 1348 Size: 2856 4708 PciI A'CATG_T Cuts at: 3814 6463 3814 Size: 2649 4915 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 3198 4636 1108 Size: 1284 806 1438 4036 Fragments arranged by size: 4036 1438 1284 806 PfoI T'CCnGG_A Cuts at: 2866 3301 4253 2866 Size: 435 952 6177 Fragments arranged by size: 6177 952 435 PmeI GTTT'AAAC Cuts at: 4868 4868 Size: 7564 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5633 5665 7146 7178 5633 Size: 32 1481 32 6019 Fragments arranged by size: 6019 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 3727 4675 2260 Size: 1467 948 5149 Fragments arranged by size: 5149 1467 948 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7564 PsiI TTA'TAA Cuts at: 5134 5134 Size: 7564 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2586 2618 2586 Size: 32 7532 PstI C_TGCA'G Cuts at: 830 3024 4916 830 Size: 2194 1892 3478 Fragments arranged by size: 3478 2194 1892 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7564 PvuII CAG'CTG Cuts at: 3539 4138 3539 Size: 599 6965 SalI G'TCGA_C Cuts at: 1936 4906 1936 Size: 2970 4594 SbfI CC_TGCA'GG Cuts at: 4916 4916 Size: 7564 ScaI AGT'ACT Cuts at: 2595 5812 7524 2595 Size: 3217 1712 2635 Fragments arranged by size: 3217 2635 1712 SfcI C'TryA_G Cuts at: 826 1045 3020 3216 3759 4696 4912 6047 6728 Size: 219 1975 196 543 937 216 1135 681 Cuts at: 6728 6919 826 Size: 191 1471 Fragments arranged by size: 1975 1471 1135 937 681 543 219 216 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7564 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7564 SmaI CCC'GGG Cuts at: 1872 4891 1872 Size: 3019 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2060 2546 3398 4670 5668 6569 Size: 197 926 117 486 852 1272 998 901 Cuts at: 6569 6831 820 Size: 262 1553 Fragments arranged by size: 1553 1272 998 926 901 852 486 262 197 117 SnaBI TAC'GTA Cuts at: 493 5355 493 Size: 4862 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7564 SphI G_CATG'C Cuts at: 1132 4922 1132 Size: 3790 3774 SspI AAT'ATT Cuts at: 5 52 5488 5 Size: 47 5436 2081 Fragments arranged by size: 5436 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7564 StyI C'CwwG_G Cuts at: 513 1065 3288 3445 3459 3550 3765 3969 4515 Size: 552 2223 157 14 91 215 204 546 Cuts at: 4515 5003 513 Size: 488 3074 Fragments arranged by size: 3074 2223 552 546 488 215 204 157 91 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3218 5591 5608 5761 5946 6391 325 Size: 1255 1638 2373 17 153 185 445 1498 Fragments arranged by size: 2373 1638 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2593 5810 7522 Size: 274 80 33 51 1443 616 3217 1712 Cuts at: 7522 96 Size: 138 Fragments arranged by size: 3217 1712 1443 616 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3133 3165 3967 3999 7524 7556 3133 Size: 32 802 32 3525 32 3141 Fragments arranged by size: 3525 3141 802 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7564 XbaI T'CTAG_A Cuts at: 2428 4900 2428 Size: 2472 5092 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2338 Size: 7564 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7564 XmaI C'CCGG_G Cuts at: 1870 4889 1870 Size: 3019 4545 XmnI GAAnn'nnTTC Cuts at: 2029 4127 5693 2029 Size: 2098 1566 3900 Fragments arranged by size: 3900 2098 1566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2488 5372 276 Size: 53 83 186 936 954 2884 2468 Fragments arranged by size: 2884 2468 954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI