Restriction map for
pFN21AE1825
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 332 GTAC GT^AC
AlwNI 682 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 771 ATTAAT AT^TAAT
AsnI 771 ATTAAT AT^TAAT
BanII 232 GGGCTC G[GA]GC[CT]^C
BbvCI 915 CCTCAGC CCTCAGC(-5/-2)
BlpI 1009 GCTCAGC GC^T[ACGT]AGC
BmyI 232 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 1009 GCTCAGC GC^T[ACGT]AGC
BsaI 1002 GAGACC GGTCTC(1/5)
BsaJI 245 CCCTGG C^C[ACGT][ACGT]GG
BseRI 883 CTCCTC GAGGAG(10/8)
BsiYI 810 CCAGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 810 CCAGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 445 GAATGC GAATGC(1/-1)
Bsp1286I 232 GGGCTC G[AGT]GC[ACT]^C
BspMI 782 ACCTGC ACCTGC(4/8)
BsrI 810 CCAGT ACTGG(1/-1)
BstXI 131 CCAAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1009 GCTCAGC GC^T[ACGT]AGC
DpnII 987 GATC ^GATC
EarI 999 GAAGAG CTCTTC(1/4)
HgaI 861 GACGC GACGC(5/10)
HincII 525 GTCAAC GT[CT]^[GA]AC
HindII 525 GTCAAC GT[CT]^[GA]AC
HindIII 691 AAGCTT A^AGCTT
HphI 700 GGTGA GGTGA(8/7)
Ksp632I 999 GAAGAG CTCTTC(1/4)
MboI 987 GATC ^GATC
MslI 729 CACAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 987 GATC ^GATC
NlaIV 233 GGCTCC GG[ACGT]^[ACGT]CC
PshAI 793 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 771 ATTAAT AT^TAAT
RsaI 332 GTAC GT^AC
Sau3AI 987 GATC ^GATC
SfcI 116 CTACAG C^T[GA][CT]AG
StuI 818 AGGCCT AGG^CCT
TaqI 518 TCGA T^CGA
TthHB8I 518 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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BbsI 320 GAAGAC GAAGAC(2/6)
966 GAAGAC
BbvI 864 GCAGC GCAGC(8/12)
786 GCTGC
BfaI 712 CTAG C^TAG
874 CTAG
BpmI 32 CTCCAG CTGGAG(16/14)
407 CTCCAG
BpuAI 320 GAAGAC GAAGAC(2/6)
966 GAAGAC
BsgI 360 CTGCAC GTGCAG(16/14)
596 GTGCAG
BssKI 246 CCTGG ^CC[ACGT]GG
894 CCAGG
BstNI 246 CCTGG CC^[AT]GG
894 CCAGG
EcoRII 246 CCTGG ^CC[AT]GG
894 CCAGG
EcoT22I 447 ATGCAT ATGCA^T
548 ATGCAT
Fnu4HI 786 GCTGC GC^[ACGT]GC
864 GCAGC
HaeIII 819 GGCC GG^CC
897 GGCC
HinfI 51 GAATC G^A[ACGT]TC
403 GAATC
ItaI 786 GCTGC GC^[ACGT]GC
864 GCAGC
MaeI 712 CTAG C^TAG
874 CTAG
MaeII 290 ACGT A^CGT
827 ACGT
MvaI 246 CCTGG CC^[AT]GG
894 CCAGG
NsiI 447 ATGCAT ATGCA^T
548 ATGCAT
ScrFI 246 CCTGG CC^[ACGT]GG
894 CCAGG
SfaNI 547 GATGC GCATC(5/9)
449 GCATC
SmlI 212 CTCAAG C^T[CT][GA]AG
886 CTCAAG
SspI 310 AATATT AAT^ATT
373 AATATT
TfiI 51 GAATC G^A[AT]TC
403 GAATC
TseI 786 GCTGC G^C[AT]GC
864 GCAGC
Tsp45I 670 GTGAC ^GT[GC]AC
684 GTCAC
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AcsI AluI ApoI BsmAI BstF5I
DdeI FokI MaeIII MboII MnlI
MseI MwoI NlaIII Tru9I Tsp509I
TspRI