################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsgI BsiEI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MslI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1065 GACGTC GACGT^C AccI 3 GTATAC GT^[AC][GT]AC AccIII 839 TCCGGA T^CCGGA AcsI 190 GAATTC [GA]^AATT[CT] AlwNI 701 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 258 GGGCCC GGGCC^C ApoI 190 GAATTC [GA]^AATT[CT] AvaI 757 CTCGGG C^[CT]CG[GA]G AviII 547 TGCGCA TGC^GCA BcnI 812 CCGGG CC^[GC]GG BpmI 561 CTGGAG CTGGAG(16/14) BsaAI 1209 CACGTA [CT]AC^GT[GA] BsaI 769 GAGACC GGTCTC(1/5) BseAI 839 TCCGGA T^CCGGA BsiWI 1211 CGTACG C^GTACG BsmBI 465 GAGACG CGTCTC(1/5) BsoBI 757 CTCGGG C^[CT]CG[GA]G BspEI 839 TCCGGA T^CCGGA BsrDI 1358 CATTGC GCAATG(2/0) Bst1107I 3 GTATAC GTA^TAC BstBI 1481 TTCGAA TT^CGAA BstYI 1385 GGATCT [GA]^GATC[CT] BstZ17I 3 GTATAC GTA^TAC EcoRI 190 GAATTC G^AATTC EcoT14I 582 CCAAGG C^C[AT][AT]GG FauI 413 GCGGG CCCGC(4/6) FspI 547 TGCGCA TGC^GCA HaeII 618 GGCGCT [GA]GCGC^[CT] KspI 204 CCGCGG CCGC^GG MaeIII 1282 GTGAC ^GT[ACGT]AC MflI 1385 GGATCT [GA]^GATC[CT] MroI 839 TCCGGA T^CCGGA MseI 281 TTAA T^TAA NciI 812 CCGGG CC^[GC]GG NdeI 1425 CATATG CA^TATG NspV 1481 TTCGAA TT^CGAA SacII 204 CCGCGG CCGC^GG SfuI 1481 TTCGAA TT^CGAA StyI 582 CCAAGG C^C[AT][AT]GG Tru9I 281 TTAA T^TAA Tsp45I 1282 GTGAC ^GT[GC]AC XhoII 1385 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 205 CGCG CG^CG 469 CGCG AcyI 1065 GACGTC G[GA]^CG[CT]C 1111 GGCGTC Alw44I 127 GTGCAC G^TGCAC 1520 GTGCAC ApaLI 127 GTGCAC G^TGCAC 1520 GTGCAC Asp700I 882 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1146 GAATGTCTTC AvaII 500 GGACC G^G[AT]CC 1366 GGACC BalI 1160 TGGCCA TGG^CCA 1313 TGGCCA BanI 293 GGTGCC G^G[CT][GA]CC 586 GGTGCC BbvCI 231 CCTCAGC CCTCAGC(-5/-2) 486 CCTCAGC BsaHI 1065 GACGTC G[GA]^CG[CT]C 1111 GGCGTC BsaWI 502 ACCGGA [AT]^CCGG[AT] 839 TCCGGA BseRI 114 CTCCTC GAGGAG(10/8) 766 GAGGAG BsmAI 465 GAGAC GTCTC(1/5) 769 GAGAC BsmI 567 GAATGC GAATGC(1/-1) 1478 GCATTC BspHI 12 TCATGA T^CATGA 623 TCATGA BspLU11I 827 ACATGT A^CATGT 1524 ACATGT BstUI 205 CGCG CG^CG 469 CGCG DsaI 204 CCGCGG C^C[GA][CT]GG 494 CCACGG EaeI 1160 TGGCCA [CT]^GGCC[GA] 1313 TGGCCA EcoNI 474 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1372 CCTTTTGCAGG HgaI 467 GACGC GACGC(5/10) 1112 GCGTC Hin1I 1065 GACGTC G[GA]^CG[CT]C 1111 GGCGTC HincII 325 GTCAAC GT[CT]^[GA]AC 714 GTCAAC HindII 325 GTCAAC GT[CT]^[GA]AC 714 GTCAAC MluNI 1160 TGGCCA TGG^CCA 1313 TGGCCA MscI 1160 TGGCCA TGG^CCA 1313 TGGCCA MvnI 205 CGCG CG^CG 469 CGCG NspI 827 ACATGT [GA]CATG^[CT] 1524 ACATGT PleI 392 GAGTC GAGTC(4/5) 875 GAGTC PvuII 398 CAGCTG CAG^CTG 858 CAGCTG RcaI 12 TCATGA T^CATGA 623 TCATGA TspRI 62 CACTG CA[GC]TG[ACGT][ACGT]^ 1254 CAGTG XmnI 882 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1146 GAATGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI BanII BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp509I TthHB8I