(Circular) (Six-base) MAPSORT of: pFN21AE6480.seq Check: 1751 from: 1 to: 6235 pFN21AE6480 6235 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3074 4045 278 Size: 53 83 186 936 1538 971 2468 Fragments arranged by size: 2468 1538 971 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 3578 1937 Size: 72 1569 4594 Fragments arranged by size: 4594 1569 72 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 2561 2873 3312 4298 5682 5950 1632 Size: 458 471 312 439 986 1384 268 1917 Fragments arranged by size: 1917 1384 986 471 458 439 312 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 2832 3213 3529 5134 6111 1203 Size: 1629 381 316 1605 977 1327 Fragments arranged by size: 1629 1605 1327 977 381 316 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 2711 3978 5550 2711 Size: 1267 1572 3396 Fragments arranged by size: 3396 1572 1267 ApaI G_GGCC'C Cuts at: 2267 2267 Size: 6235 ApaLI G'TGCA_C Cuts at: 2132 3525 4292 5448 2132 Size: 1393 767 1156 2919 Fragments arranged by size: 2919 1393 1156 767 ApoI r'AATT_y Cuts at: 1493 2195 3544 3770 3922 6031 1493 Size: 702 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 702 226 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2762 3560 1870 Size: 73 819 798 4545 Fragments arranged by size: 4545 819 798 73 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2298 2591 3556 5011 618 Size: 325 217 627 511 293 965 1455 1842 Fragments arranged by size: 1842 1455 965 627 511 325 293 217 BanII G_rGCy'C Cuts at: 1391 2153 2267 2328 2763 3554 1391 Size: 762 114 61 435 791 4072 Fragments arranged by size: 4072 791 762 435 114 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 3148 3300 928 Size: 971 962 287 152 3863 Fragments arranged by size: 3863 971 962 287 152 BbvCI CC'TCA_GC Cuts at: 2237 2492 2237 Size: 255 5980 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 1548 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6230 6230 Size: 6235 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2136 2267 2807 2823 2842 3529 4296 5452 Size: 346 131 540 16 19 687 767 1156 Cuts at: 5452 1790 Size: 2573 Fragments arranged by size: 2573 1156 767 687 540 346 131 19 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3966 4924 442 Size: 416 53 83 399 24 2549 958 1753 Fragments arranged by size: 2549 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2587 4895 1239 Size: 1348 2308 2579 Fragments arranged by size: 2579 2308 1348 Bpu10I CC'TnA_GC Cuts at: 2237 2492 2237 Size: 255 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4360 5225 5523 4360 Size: 865 298 5072 Fragments arranged by size: 5072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2768 4898 882 Size: 617 1269 2130 2219 Fragments arranged by size: 2219 2130 1269 617 BsaAI yAC'GTr Cuts at: 493 1204 3216 4026 493 Size: 711 2012 810 2702 Fragments arranged by size: 2702 2012 810 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3071 3117 4042 4424 Size: 53 83 186 936 1538 46 925 382 Cuts at: 4424 5059 275 Size: 635 1451 Fragments arranged by size: 1538 1451 936 925 635 382 186 83 53 46 BsaWI w'CCGG_w Cuts at: 2507 2844 4668 5066 5340 5487 5898 2507 Size: 337 1824 398 274 147 411 2844 Fragments arranged by size: 2844 1824 411 398 337 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 1178 Size: 30 165 30 910 30 5070 Fragments arranged by size: 5070 910 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2110 2786 1186 Size: 924 676 4635 Fragments arranged by size: 4635 924 676 BseYI C'CCAG_C Cuts at: 2343 3367 5438 5934 2343 Size: 1024 2071 496 2644 Fragments arranged by size: 2644 2071 1024 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5662 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3685 4446 5474 1540 Size: 275 186 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2136 3529 4296 4381 5452 2118 Size: 18 1393 767 85 1071 2901 Fragments arranged by size: 2901 1393 1071 767 85 18 BsiWI C'GTAC_G Cuts at: 3216 3216 Size: 6235 BsmI GAATG_Cn' Cuts at: 2578 3483 3746 3839 2578 Size: 905 263 93 4974 Fragments arranged by size: 4974 905 263 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2464 2464 Size: 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2136 2153 2267 2328 2763 2807 Size: 399 328 18 17 114 61 435 44 Cuts at: 2807 2823 2842 3529 3554 4296 4381 5452 1391 Size: 16 19 687 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 435 399 328 114 85 61 44 25 19 18 17 16 BspEI T'CCGG_A Cuts at: 2844 5898 2844 Size: 3054 3181 BspHI T'CATG_A Cuts at: 2017 2628 4124 5854 2017 Size: 611 1496 1730 2398 Fragments arranged by size: 2398 1730 1496 611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2439 2445 2796 3590 844 Size: 1595 6 351 794 3489 Fragments arranged by size: 3489 1595 794 351 6 BsrBI CCG'CTC Cuts at: 1394 1823 4122 1394 Size: 429 2299 3507 Fragments arranged by size: 3507 2299 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3362 4730 4904 65 Size: 1183 2114 1368 174 1396 Fragments arranged by size: 2114 1396 1368 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4879 5066 1700 Size: 110 3069 187 2869 Fragments arranged by size: 3069 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6235 BstBI TT'CG_AA Cuts at: 3487 5039 3487 Size: 1552 4683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstYI r'GATC_y Cuts at: 1972 3390 3565 3932 4317 4334 5786 5872 5884 Size: 1418 175 367 385 17 1452 86 12 Cuts at: 5884 6230 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1418 385 367 346 175 86 17 12 BstZ17I GTA'TAC Cuts at: 2010 2010 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 2209 2499 513 Size: 552 1144 290 4249 Fragments arranged by size: 4249 1144 552 290 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 3751 4545 4565 3751 Size: 794 20 5421 Fragments arranged by size: 5421 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3165 3318 3682 Size: 54 1425 50 275 18 1335 153 364 Cuts at: 3682 4571 6200 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1335 889 364 275 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 2462 2739 4165 1353 Size: 1034 75 277 1426 3423 Fragments arranged by size: 3423 1426 1034 277 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2109 4822 5196 5342 387 Size: 1064 138 520 2713 374 146 1280 Fragments arranged by size: 2713 1280 1064 520 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2561 2587 2873 3312 4298 4895 Size: 393 458 471 26 286 439 986 597 Cuts at: 4895 5682 5950 1239 Size: 787 268 1524 Fragments arranged by size: 1524 986 787 597 471 458 439 393 286 268 26 EcoNI CCTnn'n_nnAGG Cuts at: 2483 3381 2483 Size: 898 5337 EcoO109I rG'GnC_Cy Cuts at: 2264 2488 2634 2264 Size: 224 146 5865 Fragments arranged by size: 5865 224 146 EcoRI G'AATT_C Cuts at: 2195 3544 3922 2195 Size: 1349 378 4508 Fragments arranged by size: 4508 1349 378 FspI TGC'GCA Cuts at: 2554 4741 2554 Size: 2187 4048 HaeII r_GCGC'y Cuts at: 1678 2627 5382 1678 Size: 949 2755 2531 Fragments arranged by size: 2755 2531 949 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2313 2345 2463 2495 2914 Size: 32 135 32 741 32 118 32 419 Cuts at: 2914 2946 4876 4908 4950 4982 1373 Size: 32 1930 32 42 32 2626 Fragments arranged by size: 2626 1930 741 419 135 118 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2332 2721 3579 3825 669 Size: 988 281 394 389 858 246 3079 Fragments arranged by size: 3079 988 858 394 389 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5349 5533 1305 Size: 4044 184 2007 Fragments arranged by size: 4044 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3167 3320 10 Size: 54 1425 343 1335 153 2925 Fragments arranged by size: 2925 1425 1335 343 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 4193 4552 4711 518 Size: 648 3027 359 159 2042 Fragments arranged by size: 3027 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2211 2405 2418 2438 2865 3651 4328 5476 Size: 605 194 13 20 427 786 677 1148 Cuts at: 5476 5721 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 786 677 605 427 245 194 20 13 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5683 NdeI CA'TA_TG Cuts at: 387 3431 387 Size: 3044 3191 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 2836 3533 3593 5138 1132 Size: 97 1607 697 60 1545 2229 Fragments arranged by size: 2229 1607 1545 697 97 60 PasI CC'CwG_GG Cuts at: 1348 2164 2338 2485 1348 Size: 816 174 147 5098 Fragments arranged by size: 5098 816 174 147 PciI A'CATG_T Cuts at: 2832 3529 5134 2832 Size: 697 1605 3933 Fragments arranged by size: 3933 1605 697 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6235 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 2263 2263 Size: 6235 PstI C_TGCA'G Cuts at: 830 3587 830 Size: 2757 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2405 2865 2405 Size: 460 5775 SacII CC_GC'GG Cuts at: 2212 2212 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SfcI C'TryA_G Cuts at: 826 1045 3583 4718 5399 5590 826 Size: 219 2538 1135 681 191 1471 Fragments arranged by size: 2538 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4339 5240 5502 820 Size: 197 926 2396 901 262 1553 Fragments arranged by size: 2396 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2587 3674 513 Size: 552 1522 1087 3074 Fragments arranged by size: 3074 1522 1087 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2094 2156 2286 2335 4262 4279 4432 Size: 1255 514 62 130 49 1927 17 153 Cuts at: 4432 4617 5062 325 Size: 185 445 1498 Fragments arranged by size: 1927 1498 1255 514 445 185 153 130 62 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4481 6193 96 Size: 274 80 33 51 1443 2504 1712 138 Fragments arranged by size: 2504 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6195 6227 6195 Size: 32 6203 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 2891 3155 4364 2891 Size: 264 1209 4762 Fragments arranged by size: 4762 1209 264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3072 4043 276 Size: 53 83 186 936 1538 971 2468 Fragments arranged by size: 2468 1538 971 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI