(Linear) (Six-base) MAPSORT of: KIEE6480.seq Check: 7303 from: 1 to: 1530 KIEE6480.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AatII G_ACGT'C Cuts at: 0 1069 1530 Size: 1069 461 AccI GT'mk_AC Cuts at: 0 4 1530 Size: 4 1526 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 556 868 1307 1530 Size: 85 471 312 439 223 Fragments arranged by size: 471 439 312 223 85 AflIII A'CryG_T Cuts at: 0 827 1208 1524 1530 Size: 827 381 316 6 Fragments arranged by size: 827 381 316 6 AlwNI CAG_nnn'CTG Cuts at: 0 706 1530 Size: 706 824 ApaI G_GGCC'C Cuts at: 0 262 1530 Size: 262 1268 ApaLI G'TGCA_C Cuts at: 0 127 1520 1530 Size: 127 1393 10 Fragments arranged by size: 1393 127 10 ApoI r'AATT_y Cuts at: 0 190 1530 Size: 190 1340 AvaI C'yCGr_G Cuts at: 0 757 1530 Size: 757 773 BanI G'GyrC_C Cuts at: 0 293 586 1530 Size: 293 293 944 Fragments arranged by size: 944 293 293 BanII G_rGCy'C Cuts at: 0 148 262 323 758 1530 Size: 148 114 61 435 772 Fragments arranged by size: 772 435 148 114 61 BbsI GAAGACnn'nnnn_ Cuts at: 0 856 1143 1295 1530 Size: 856 287 152 235 Fragments arranged by size: 856 287 235 152 BbvCI CC'TCA_GC Cuts at: 0 232 487 1530 Size: 232 255 1043 Fragments arranged by size: 1043 255 232 Bme1580I G_kGCm'C Cuts at: 0 131 262 802 818 837 1524 1530 Size: 131 131 540 16 19 687 6 Fragments arranged by size: 687 540 131 131 19 16 6 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 582 1530 Size: 582 948 Bpu10I CC'TnA_GC Cuts at: 0 232 487 1530 Size: 232 255 1043 Fragments arranged by size: 1043 255 232 BsaI GGTCTCn'nnnn_ Cuts at: 0 763 1530 Size: 763 767 BsaAI yAC'GTr Cuts at: 0 1211 1530 Size: 1211 319 BsaHI Gr'CG_yC Cuts at: 0 1066 1112 1530 Size: 1066 46 418 Fragments arranged by size: 1066 418 46 BsaWI w'CCGG_w Cuts at: 0 502 839 1530 Size: 502 337 691 Fragments arranged by size: 691 502 337 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 308 338 1530 Size: 308 30 1192 Fragments arranged by size: 1192 308 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 105 781 1530 Size: 105 676 749 Fragments arranged by size: 749 676 105 BseYI C'CCAG_C Cuts at: 0 338 1362 1530 Size: 338 1024 168 Fragments arranged by size: 1024 338 168 BsiHKAI G_wGCw'C Cuts at: 0 113 131 1524 1530 Size: 113 18 1393 6 Fragments arranged by size: 1393 113 18 6 BsiWI C'GTAC_G Cuts at: 0 1211 1530 Size: 1211 319 BsmI GAATG_Cn' Cuts at: 0 573 1478 1530 Size: 573 905 52 Fragments arranged by size: 905 573 52 BsmBI CGTCTCn'nnnn_ Cuts at: 0 459 1530 Size: 459 1071 Bsp1286I G_dGCh'C Cuts at: 0 113 131 148 262 323 758 802 818 Size: 113 18 17 114 61 435 44 16 Cuts at: 818 837 1524 1530 Size: 19 687 6 Fragments arranged by size: 687 435 114 113 61 44 19 18 17 16 6 BspEI T'CCGG_A Cuts at: 0 839 1530 Size: 839 691 BspHI T'CATG_A Cuts at: 0 12 623 1530 Size: 12 611 907 Fragments arranged by size: 907 611 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 434 440 791 1530 Size: 434 6 351 739 Fragments arranged by size: 739 434 351 6 BsrDI GCAATG_nn' Cuts at: 0 1357 1530 Size: 1357 173 BstBI TT'CG_AA Cuts at: 0 1482 1530 Size: 1482 48 BstYI r'GATC_y Cuts at: 0 1385 1530 Size: 1385 145 BstZ17I GTA'TAC Cuts at: 0 5 1530 Size: 5 1525 BtgI C'CryG_G Cuts at: 0 204 494 1530 Size: 204 290 1036 Fragments arranged by size: 1036 290 204 EaeI y'GGCC_r Cuts at: 0 1160 1313 1530 Size: 1160 153 217 Fragments arranged by size: 1160 217 153 EarI CTCTTCn'nnn_ Cuts at: 0 382 457 734 1530 Size: 382 75 277 796 Fragments arranged by size: 796 382 277 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 104 1530 Size: 104 1426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 556 582 868 1307 1530 Size: 85 471 26 286 439 223 Fragments arranged by size: 471 439 286 223 85 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 478 1376 1530 Size: 478 898 154 Fragments arranged by size: 898 478 154 EcoO109I rG'GnC_Cy Cuts at: 0 259 483 629 1530 Size: 259 224 146 901 Fragments arranged by size: 901 259 224 146 EcoRI G'AATT_C Cuts at: 0 190 1530 Size: 190 1340 FspI TGC'GCA Cuts at: 0 549 1530 Size: 549 981 HaeII r_GCGC'y Cuts at: 0 622 1530 Size: 622 908 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 308 340 458 490 909 941 1530 Size: 308 32 118 32 419 32 589 Fragments arranged by size: 589 419 308 118 32 32 32 HincII GTy'rAC Cuts at: 0 327 716 1530 Size: 327 389 814 Fragments arranged by size: 814 389 327 MscI TGG'CCA Cuts at: 0 1162 1315 1530 Size: 1162 153 215 Fragments arranged by size: 1162 215 153 MspA1I CmG'CkG Cuts at: 0 206 400 413 433 860 1530 Size: 206 194 13 20 427 670 Fragments arranged by size: 670 427 206 194 20 13 NdeI CA'TA_TG Cuts at: 0 1426 1530 Size: 1426 104 NspI r_CATG'y Cuts at: 0 831 1528 1530 Size: 831 697 2 Fragments arranged by size: 831 697 2 PasI CC'CwG_GG Cuts at: 0 159 333 480 1530 Size: 159 174 147 1050 Fragments arranged by size: 1050 174 159 147 PciI A'CATG_T Cuts at: 0 827 1524 1530 Size: 827 697 6 Fragments arranged by size: 827 697 6 PspOMI G'GGCC_C Cuts at: 0 258 1530 Size: 258 1272 PvuII CAG'CTG Cuts at: 0 400 860 1530 Size: 400 460 670 Fragments arranged by size: 670 460 400 SacII CC_GC'GG Cuts at: 0 207 1530 Size: 207 1323 StyI C'CwwG_G Cuts at: 0 582 1530 Size: 582 948 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 89 151 281 330 1530 Size: 89 62 130 49 1200 Fragments arranged by size: 1200 130 89 62 49 XmnI GAAnn'nnTTC Cuts at: 0 886 1150 1530 Size: 886 264 380 Fragments arranged by size: 886 380 264 ZraI GAC'GTC Cuts at: 0 1067 1530 Size: 1067 463 Enzymes that do cut: AatII AccI AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BstBI BstYI BstZ17I BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII MscI MspA1I NdeI NspI PasI PciI PspOMI PvuII SacII StyI TaqII XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaBI BsgI BsiEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI