Restriction map for
pFN21AE4013
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstF5I BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRV EcoT14I EcoT22I FbaI
FokI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI TspRI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 417 GTCGAC GT^[AC][GT]AC
AcyI 3 GGCGTC G[GA]^CG[CT]C
AlwI 327 GGATC GGATC(4/5)
AlwNI 73 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 449 GGGCCC GGGCC^C
AspI 241 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 449 GGGCCC G[GA]GC[CT]^C
BbsI 175 GAAGAC GAAGAC(2/6)
BcnI 86 CCCGG CC^[GC]GG
BglII 116 AGATCT A^GATCT
BmyI 449 GGGCCC G[AGT]GC[ACT]^C
BpuAI 175 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaI 89 GGTCTC GGTCTC(1/5)
BsaJI 452 CCCTGG C^C[ACGT][ACGT]GG
Bsp1286I 449 GGGCCC G[AGT]GC[ACT]^C
BspDI 137 ATCGAT AT^CGAT
BspMI 421 ACCTGC ACCTGC(4/8)
BstEII 81 GGTCACC G^GT[ACGT]ACC
BstPI 81 GGTCACC G^GT[ACGT]ACC
Cac8I 47 GCCCGC GC[ACGT]^[ACGT]GC
ClaI 137 ATCGAT AT^CGAT
EcoNI 453 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 81 GGTCACC G^GT[ACGT]ACC
EcoRI 189 GAATTC G^AATTC
FauI 48 CCCGC CCCGC(4/6)
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HincII 417 GTCGAC GT[CT]^[GA]AC
HindII 417 GTCGAC GT[CT]^[GA]AC
HinfI 21 GACTC G^A[ACGT]TC
MaeII 443 ACGT A^CGT
MseI 217 TTAA T^TAA
MwoI 504 GCTCCAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 86 CCCGG CC^[GC]GG
NlaIV 449 GGGCCC GG[ACGT]^[ACGT]CC
NspI 383 GCATGT [GA]CATG^[CT]
PflFI 241 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 72 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 21 GACTC GAGTC(4/5)
PvuII 478 CAGCTG CAG^CTG
SalI 417 GTCGAC G^TCGAC
SfcI 35 CTGTAG C^T[GA][CT]AG
Tru9I 217 TTAA T^TAA
Tsp45I 82 GTCAC ^GT[GC]AC
Tth111I 241 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 72 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 189 GAATTC [GA]^AATT[CT]
268 AAATTT
ApoI 189 GAATTC [GA]^AATT[CT]
268 AAATTT
BbvI 513 GCAGC GCAGC(8/12)
12 GCTGC
BfaI 56 CTAG C^TAG
184 CTAG
BstYI 116 AGATCT [GA]^GATC[CT]
327 GGATCT
DraII 448 AGGGCCC [GA]G^G[ACGT]CC[CT]
449 GGGCCCT
EcoO109I 448 AGGGCCC [GA]G^G[ACGT]CC[CT]
449 GGGCCCT
HaeIII 46 GGCC GG^CC
450 GGCC
HapII 16 CCGG C^CGG
87 CCGG
HpaII 16 CCGG C^CGG
87 CCGG
HphI 83 TCACC GGTGA(8/7)
488 TCACC
MaeI 56 CTAG C^TAG
184 CTAG
MaeIII 75 GTTAC ^GT[ACGT]AC
82 GTCAC
MflI 116 AGATCT [GA]^GATC[CT]
327 GGATCT
MspA1I 478 CAGCTG C[AC]G^C[GT]G
508 CAGCGG
MspI 16 CCGG C^CGG
87 CCGG
NlaIII 172 CATG CATG^
384 CATG
SspI 219 AATATT AAT^ATT
234 AATATT
TaqI 138 TCGA T^CGA
418 TCGA
TseI 12 GCTGC G^C[AT]GC
513 GCAGC
TthHB8I 138 TCGA T^CGA
418 TCGA
XhoII 116 AGATCT [GA]^GATC[CT]
327 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BpmI BsiYI BslI
BsmAI BsrI BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI ItaI
MboI MboII MnlI MvaI NdeII
Sau3AI Sau96I ScrFI Tsp509I