################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BseRI BsiEI BsiWI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MfeI MluI MluNI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 449 TCCGGA T^CCGGA AcsI 1002 AAATTT [GA]^AATT[CT] AfaI 750 GTAC GT^AC AflIII 980 ACATGT A^C[GA][CT]GT AhdI 7 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 1002 AAATTT [GA]^AATT[CT] AspEI 7 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 888 GGTGCC G^G[CT][GA]CC BanII 816 GAGCTC G[GA]GC[CT]^C BcgI 1123 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 124 CTAG C^TAG BglII 549 AGATCT A^GATCT BsaBI 876 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 449 TCCGGA T^CCGGA BsgI 901 GTGCAG GTGCAG(16/14) BsmBI 333 CGTCTC CGTCTC(1/5) BsmFI 543 GTCCC GGGAC(10/14) BsmI 387 GAATGC GAATGC(1/-1) BspEI 449 TCCGGA T^CCGGA BspHI 177 TCATGA T^CATGA BspLU11I 980 ACATGT A^CATGT BsrDI 1119 CATTGC GCAATG(2/0) DdeI 812 CTGAG C^T[ACGT]AG Eam1105I 7 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 55 CTCTTC CTCTTC(1/4) EcoT22I 497 ATGCAT ATGCA^T HincII 723 GTCGAC GT[CT]^[GA]AC HindII 723 GTCGAC GT[CT]^[GA]AC HindIII 1091 AAGCTT A^AGCTT Ksp632I 55 CTCTTC CTCTTC(1/4) MaeI 124 CTAG C^TAG MamI 876 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 449 TCCGGA T^CCGGA NsiI 497 ATGCAT ATGCA^T PaeR7I 115 CTCGAG C^TCGAG PflMI 35 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 9 CTGCAG CTGCA^G RcaI 177 TCATGA T^CATGA RsaI 750 GTAC GT^AC SacI 816 GAGCTC GAGCT^C SalI 723 GTCGAC G^TCGAC SmlI 115 CTCGAG C^T[CT][GA]AG SphI 791 GCATGC GCATG^C SspI 306 AATATT AAT^ATT StuI 172 AGGCCT AGG^CCT Van91I 35 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 115 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 723 GTCGAC GT^[AC][GT]AC 1150 GTAGAC AlwNI 473 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 1190 CAGGTGCTG AspHI 565 GTGCTC G[AT]GC[AT]^C 816 GAGCTC AvaI 115 CTCGAG C^[CT]CG[GA]G 631 CCCGAG BamHI 140 GGATCC G^GATCC 468 GGATCC BsaWI 449 TCCGGA [AT]^CCGG[AT] 713 TCCGGT BsiHKAI 565 GTGCTC G[AT]GC[AT]^C 816 GAGCTC BsmAI 111 GTCTC GTCTC(1/5) 334 GTCTC BsoBI 115 CTCGAG C^[CT]CG[GA]G 631 CCCGAG BspMI 11 GCAGGT ACCTGC(4/8) 903 GCAGGT BstAPI 1000 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1102 GCAACAACTGC HapII 450 CCGG C^CGG 714 CCGG HgaI 75 GACGC GACGC(5/10) 211 GCGTC HinfI 487 GATTC G^A[ACGT]TC 988 GATTC HpaII 450 CCGG C^CGG 714 CCGG MspI 450 CCGG C^CGG 714 CCGG NcoI 201 CCATGG C^CATGG 269 CCATGG NspI 791 GCATGC [GA]CATG^[CT] 980 ACATGT TfiI 487 GATTC G^A[AT]TC 988 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII