(Circular) (Six-base) MAPSORT of: pFN21AE1883.seq Check: 4384 from: 1 to: 5932 pFN21AE1883 5932 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2899 2899 Size: 5932 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3742 278 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 3156 3275 1937 Size: 792 427 119 4594 Fragments arranged by size: 4594 792 427 119 Acc65I G'GTAC_C Cuts at: 3253 3253 Size: 5932 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 3084 3995 5379 5647 1632 Size: 587 865 911 1384 268 1917 Fragments arranged by size: 1917 1384 911 865 587 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 2985 4831 5808 1203 Size: 1782 1846 977 1327 Fragments arranged by size: 1846 1782 1327 977 AgeI A'CCGG_T Cuts at: 4763 4763 Size: 5932 AhdI GACnn_n'nnGTC Cuts at: 2017 4661 2017 Size: 2644 3288 AleI CACnn'nnGTG Cuts at: 1166 2660 1166 Size: 1494 4438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2813 2909 1184 Size: 1629 96 4207 Fragments arranged by size: 4207 1629 96 AlwNI CAG_nnn'CTG Cuts at: 2483 3200 3675 5247 2483 Size: 717 475 1572 3168 Fragments arranged by size: 3168 1572 717 475 ApaLI G'TGCA_C Cuts at: 3989 5145 3989 Size: 1156 4776 ApoI r'AATT_y Cuts at: 1493 3007 3241 3467 3619 5728 1493 Size: 1514 234 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 234 226 152 AseI AT'TA_AT Cuts at: 160 1032 4486 160 Size: 872 3454 1606 Fragments arranged by size: 3454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2636 3257 1870 Size: 73 177 516 621 4545 Fragments arranged by size: 4545 621 516 177 73 BamHI G'GATC_C Cuts at: 2145 2473 3262 3629 2145 Size: 328 789 367 4448 Fragments arranged by size: 4448 789 367 328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2893 3253 4708 618 Size: 325 217 627 1106 360 1455 1842 Fragments arranged by size: 1842 1455 1106 627 360 325 217 BanII G_rGCy'C Cuts at: 1391 2825 3251 1391 Size: 1434 426 4072 Fragments arranged by size: 4072 1434 426 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4961 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3117 3151 4123 4157 1903 Size: 34 1180 34 972 34 3678 Fragments arranged by size: 3678 1180 972 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3824 5034 3824 Size: 1210 4722 BclI T'GATC_A Cuts at: 1548 1548 Size: 5932 BfrBI ATG'CAT Cuts at: 2504 2504 Size: 5932 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4543 136 Size: 107 122 71 631 605 2871 1525 Fragments arranged by size: 2871 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2554 5927 2554 Size: 3373 2559 BlpI GC'TnA_GC Cuts at: 3349 3349 Size: 5932 Bme1580I G_kGCm'C Cuts at: 1790 2740 2898 3993 5149 1790 Size: 950 158 1095 1156 2573 Fragments arranged by size: 2573 1156 1095 950 158 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5932 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3663 4621 442 Size: 416 53 83 399 24 2246 958 1753 Fragments arranged by size: 2246 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2844 2844 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4592 1239 Size: 3353 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4057 4922 5220 4057 Size: 865 298 4769 Fragments arranged by size: 4769 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4595 882 Size: 617 3096 2219 Fragments arranged by size: 3096 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3723 493 Size: 711 2519 2702 Fragments arranged by size: 2702 2519 711 BsaBI GATnn'nnATC Cuts at: 2885 5926 2885 Size: 3041 2891 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3739 4121 4756 275 Size: 53 83 186 936 2206 382 635 1451 Fragments arranged by size: 2206 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2454 2718 4365 4763 5037 5184 5595 2454 Size: 264 1647 398 274 147 411 2791 Fragments arranged by size: 2791 1647 411 398 274 264 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5707 Fragments arranged by size: 5707 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5932 BseYI C'CCAG_C Cuts at: 2239 2703 5135 5631 2239 Size: 464 2432 496 2540 Fragments arranged by size: 2540 2432 496 464 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2927 1163 Size: 573 1191 4168 Fragments arranged by size: 4168 1191 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2574 2825 3993 4078 5149 2574 Size: 251 1168 85 1071 3357 Fragments arranged by size: 3357 1168 1071 251 85 BsmI GAATG_Cn' Cuts at: 2398 3443 3536 2398 Size: 1045 93 4794 Fragments arranged by size: 4794 1045 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2344 2344 Size: 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2574 2740 2825 2898 3251 3993 4078 Size: 399 784 166 85 73 353 742 85 Cuts at: 4078 5149 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 784 742 399 353 166 85 85 73 BspEI T'CCGG_A Cuts at: 2454 5595 2454 Size: 3141 2791 BspHI T'CATG_A Cuts at: 2182 3821 5551 2182 Size: 1639 1730 2563 Fragments arranged by size: 2563 1730 1639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2007 2899 3287 844 Size: 1163 892 388 3489 Fragments arranged by size: 3489 1163 892 388 BsrBI CCG'CTC Cuts at: 1394 1823 3819 1394 Size: 429 1996 3507 Fragments arranged by size: 3507 1996 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3123 4427 4601 65 Size: 1183 1875 1304 174 1396 Fragments arranged by size: 1875 1396 1304 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4576 4763 1700 Size: 110 2766 187 2869 Fragments arranged by size: 2869 2766 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5932 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 3992 5004 3992 Size: 1012 4920 BstAPI GCAn_nnn'nTGC Cuts at: 794 3011 3113 794 Size: 2217 102 3613 Fragments arranged by size: 3613 2217 102 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5932 BstYI r'GATC_y Cuts at: 1972 2145 2473 2554 3262 3629 4014 4031 5483 Size: 173 328 81 708 367 385 17 1452 Cuts at: 5483 5569 5581 5927 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 708 385 367 346 328 173 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2206 2274 2779 513 Size: 552 1141 68 505 3666 Fragments arranged by size: 3666 1141 552 505 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3120 487 Size: 683 56 1894 3299 Fragments arranged by size: 3299 1894 683 56 BtsI GCAGTG_nn' Cuts at: 2604 2807 3448 4242 4262 2604 Size: 203 641 794 20 4274 Fragments arranged by size: 4274 794 641 203 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5932 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4939 809 Size: 507 3623 1802 Fragments arranged by size: 3623 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897 Size: 54 1425 50 275 18 1549 889 1629 Cuts at: 5897 8 Size: 43 Fragments arranged by size: 1629 1549 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 3862 1353 Size: 713 1796 3423 Fragments arranged by size: 3423 1796 713 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2261 4519 4893 5039 387 Size: 1064 138 672 2258 374 146 1280 Fragments arranged by size: 2258 1280 1064 672 374 146 138 EcoICRI GAG'CTC Cuts at: 2823 2823 Size: 5932 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 3084 3995 4592 5379 5647 1239 Size: 393 587 865 911 597 787 268 1524 Fragments arranged by size: 1524 911 865 787 597 587 393 268 EcoRI G'AATT_C Cuts at: 3241 3619 3241 Size: 378 5554 FspI TGC'GCA Cuts at: 4438 4438 Size: 5932 HaeII r_GCGC'y Cuts at: 1678 5079 1678 Size: 3401 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2465 2497 2524 Size: 32 135 32 431 32 430 32 27 Cuts at: 2524 2556 2571 2603 2663 2695 4573 4605 4647 Size: 32 15 32 60 32 1878 32 42 Cuts at: 4647 4679 1373 Size: 32 2626 Fragments arranged by size: 2626 1878 431 430 135 60 42 32 32 32 32 32 32 32 32 32 27 15 HincII GTy'rAC Cuts at: 669 1657 1938 2730 3276 3522 669 Size: 988 281 792 546 246 3079 Fragments arranged by size: 3079 988 792 546 281 246 HindIII A'AGCT_T Cuts at: 748 3096 748 Size: 2348 3584 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KpnI G_GTAC'C Cuts at: 3257 3257 Size: 5932 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 2656 3205 5046 5230 1305 Size: 855 496 549 1841 184 2007 Fragments arranged by size: 2007 1841 855 549 496 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4110 Fragments arranged by size: 4110 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 2660 2795 2891 3890 4249 4408 Size: 648 1113 381 135 96 999 359 159 Cuts at: 4408 518 Size: 2042 Fragments arranged by size: 2042 1113 999 648 381 359 159 135 96 MspA1I CmG'CkG Cuts at: 1606 2239 2833 2877 2933 2962 3348 4025 5173 Size: 633 594 44 56 29 386 677 1148 Cuts at: 5173 5418 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 633 594 386 245 56 44 29 NcoI C'CATG_G Cuts at: 513 1065 2206 2274 513 Size: 552 1141 68 4171 Fragments arranged by size: 4171 1141 552 68 NdeI CA'TA_TG Cuts at: 387 387 Size: 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NsiI A_TGCA'T Cuts at: 2506 2506 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 2800 2989 3290 4835 1132 Size: 97 1571 189 301 1545 2229 Fragments arranged by size: 2229 1571 1545 301 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5932 PciI A'CATG_T Cuts at: 2985 4831 2985 Size: 1846 4086 PflMI CCAn_nnn'nTGG Cuts at: 1108 2046 1108 Size: 938 4994 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5932 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PstI C_TGCA'G Cuts at: 830 2018 3284 830 Size: 1188 1266 3478 Fragments arranged by size: 3478 1266 1188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 PvuII CAG'CTG Cuts at: 2239 2877 2933 2239 Size: 638 56 5238 Fragments arranged by size: 5238 638 56 SacI G_AGCT'C Cuts at: 2825 2825 Size: 5932 SalI G'TCGA_C Cuts at: 1936 2728 3274 1936 Size: 792 546 4594 Fragments arranged by size: 4594 792 546 SbfI CC_TGCA'GG Cuts at: 3284 3284 Size: 5932 ScaI AGT'ACT Cuts at: 4180 5892 4180 Size: 1712 4220 SfcI C'TryA_G Cuts at: 826 1045 2014 2934 3192 3280 4415 5096 5287 Size: 219 969 920 258 88 1135 681 191 Cuts at: 5287 826 Size: 1471 Fragments arranged by size: 1471 1135 969 920 681 258 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5932 SmaI CCC'GGG Cuts at: 1872 3259 1872 Size: 1387 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 4036 4937 5199 820 Size: 197 926 177 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 177 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 2800 3290 1132 Size: 1668 490 3774 Fragments arranged by size: 3774 1668 490 SspI AAT'ATT Cuts at: 5 52 2313 3856 5 Size: 47 2261 1543 2081 Fragments arranged by size: 2261 2081 1543 47 StuI AGG'CCT Cuts at: 1517 2179 1517 Size: 662 5270 StyI C'CwwG_G Cuts at: 513 1065 2025 2206 2274 3371 513 Size: 552 960 181 68 1097 3074 Fragments arranged by size: 3074 1097 960 552 181 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2209 3959 3976 4129 4314 4759 325 Size: 1255 629 1750 17 153 185 445 1498 Fragments arranged by size: 1750 1498 1255 629 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4178 5890 96 Size: 274 80 33 51 1443 2201 1712 138 Fragments arranged by size: 2201 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5892 5924 5892 Size: 32 5900 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5932 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XhoI C'TCGA_G Cuts at: 1943 2120 1943 Size: 177 5755 XmaI C'CCGG_G Cuts at: 1870 3257 1870 Size: 1387 4545 XmnI GAAnn'nnTTC Cuts at: 4061 4061 Size: 5932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3740 276 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI