(Circular) (Six-base) MAPSORT of: pFN21AB5921.seq Check: 7715 from: 1 to: 6220 pFN21AB5921 6220 bp With 182 enzymes: SgfI * March 28, 2012 12:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2317 2317 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 2271 3208 3541 2271 Size: 937 333 4950 Fragments arranged by size: 4950 937 333 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4283 5667 5935 1632 Size: 2651 1384 268 1917 Fragments arranged by size: 2651 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 3477 5119 6096 1203 Size: 2274 1642 977 1327 Fragments arranged by size: 2274 1642 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 3275 1166 Size: 2109 4111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2171 1184 Size: 987 5233 AlwNI CAG_nnn'CTG Cuts at: 3963 5535 3963 Size: 1572 4648 ApaI G_GGCC'C Cuts at: 2051 3132 2051 Size: 1081 5139 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2486 3529 3755 3907 6016 1493 Size: 993 1043 226 152 2109 1697 Fragments arranged by size: 2109 1697 1043 993 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2050 2380 2787 2876 3545 1870 Size: 73 107 330 407 89 669 4545 Fragments arranged by size: 4545 669 407 330 107 89 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2271 2300 3208 3541 4996 Size: 325 217 627 484 29 908 333 1455 Cuts at: 4996 618 Size: 1842 Fragments arranged by size: 1842 1455 908 627 484 333 325 217 29 BanII G_rGCy'C Cuts at: 1391 2051 3132 3539 1391 Size: 660 1081 407 4072 Fragments arranged by size: 4072 1081 660 407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3513 928 Size: 971 1614 3635 Fragments arranged by size: 3635 1614 971 BbvCI CC'TCA_GC Cuts at: 2593 2593 Size: 6220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2768 4831 136 Size: 107 122 71 631 605 1096 2063 1525 Fragments arranged by size: 2063 1525 1096 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2031 2051 3132 4281 5437 1790 Size: 241 20 1081 1149 1156 2573 Fragments arranged by size: 2573 1156 1149 1081 241 20 BmgBI CAC'GTC Cuts at: 1318 2344 1318 Size: 1026 5194 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2302 2608 2302 Size: 306 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 2726 3533 4880 1239 Size: 1478 9 807 1347 2579 Fragments arranged by size: 2579 1478 1347 807 9 Bpu10I CC'TnA_GC Cuts at: 2579 2593 2579 Size: 14 6206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2402 3425 4345 5210 5508 2402 Size: 1023 920 865 298 3114 Fragments arranged by size: 3114 1023 920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2924 4883 882 Size: 617 1425 1959 2219 Fragments arranged by size: 2219 1959 1425 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3224 3254 1178 Size: 30 165 30 1821 30 4144 Fragments arranged by size: 4144 1821 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 2522 1186 Size: 1333 3 4884 Fragments arranged by size: 4884 1333 3 BseYI C'CCAG_C Cuts at: 2394 2837 2975 3074 5423 5919 2394 Size: 443 138 99 2349 496 2695 Fragments arranged by size: 2695 2349 496 443 138 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2287 1163 Size: 573 551 5096 Fragments arranged by size: 5096 573 551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2045 3670 4431 5459 1540 Size: 275 186 44 1625 761 1028 2301 Fragments arranged by size: 2301 1625 1028 761 275 186 44 BsiHKAI G_wGCw'C Cuts at: 4281 4366 5437 4281 Size: 85 1071 5064 Fragments arranged by size: 5064 1071 85 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 BsmBI CGTCTCn'nnnn_ Cuts at: 2257 2257 Size: 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2051 3132 3539 4281 4366 5437 Size: 399 241 20 1081 407 742 85 1071 Cuts at: 5437 1391 Size: 2174 Fragments arranged by size: 2174 1081 1071 742 407 399 241 85 20 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2317 3575 844 Size: 1415 58 1258 3489 Fragments arranged by size: 3489 1415 1258 58 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3166 4715 4889 65 Size: 1183 1918 1549 174 1396 Fragments arranged by size: 1918 1549 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 4864 5051 1700 Size: 110 208 2846 187 2869 Fragments arranged by size: 2869 2846 208 187 110 BsrGI T'GTAC_A Cuts at: 96 3019 96 Size: 2923 3297 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 2282 2282 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2259 2971 3550 3917 4302 4319 5771 5857 Size: 287 712 579 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 712 579 385 367 346 287 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3174 3174 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2197 2242 3199 3273 513 Size: 552 1132 45 957 74 3460 Fragments arranged by size: 3460 1132 957 552 74 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2892 3499 3736 4530 4550 2892 Size: 607 237 794 20 4562 Fragments arranged by size: 4562 794 607 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3184 3219 3475 3510 529 Size: 35 2620 35 256 35 3239 Fragments arranged by size: 3239 2620 256 35 35 35 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2042 2104 Size: 54 1425 50 275 18 186 26 62 Cuts at: 2104 3282 3667 4556 6185 8 Size: 1178 385 889 1629 43 Fragments arranged by size: 1629 1425 1178 889 385 275 186 62 54 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2042 3667 1537 Size: 275 230 1625 4090 Fragments arranged by size: 4090 1625 275 230 EarI CTCTTCn'nnn_ Cuts at: 1353 2496 3308 3495 4150 1353 Size: 1143 812 187 655 3423 Fragments arranged by size: 3423 1143 812 655 187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 4807 5181 5327 387 Size: 1064 138 570 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 570 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2717 2726 3533 4283 4880 5667 5935 Size: 393 1085 9 807 750 597 787 268 Cuts at: 5935 1239 Size: 1524 Fragments arranged by size: 1524 1085 807 787 750 597 393 268 9 EcoNI CCTnn'n_nnAGG Cuts at: 2105 3393 2105 Size: 1288 4932 EcoO109I rG'GnC_Cy Cuts at: 3128 3129 3128 Size: 1 6219 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 EcoRV GAT'ATC Cuts at: 3451 3451 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2025 5367 1678 Size: 347 3342 2531 Fragments arranged by size: 3342 2531 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2612 2632 2644 2781 Size: 32 135 32 1028 12 20 12 137 Cuts at: 2781 2813 3041 3073 4861 4893 4935 4967 1373 Size: 32 228 32 1788 32 42 32 2626 Fragments arranged by size: 2626 1788 1028 228 137 135 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2119 3564 3810 669 Size: 988 281 181 1445 246 3079 Fragments arranged by size: 3079 1445 988 281 246 181 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 2275 3212 3545 2275 Size: 937 333 4950 Fragments arranged by size: 4950 937 333 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2907 3066 3159 5334 5518 1305 Size: 1602 159 93 2175 184 2007 Fragments arranged by size: 2175 2007 1602 184 159 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 3275 4178 4537 4696 518 Size: 648 1731 378 903 359 159 2042 Fragments arranged by size: 2042 1731 903 648 378 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2155 2230 2394 2695 2923 3288 3636 Size: 477 72 75 164 301 228 365 348 Cuts at: 3636 4313 5461 5706 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 477 365 348 301 245 228 164 75 72 NaeI GCC'GGC Cuts at: 2020 2020 Size: 6220 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 3309 387 Size: 2922 3298 NgoMIV G'CCGG_C Cuts at: 2018 2018 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2389 3578 5123 1132 Size: 97 1160 1189 1545 2229 Fragments arranged by size: 2229 1545 1189 1160 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2047 3128 2047 Size: 1081 5139 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2625 2657 2625 Size: 32 6188 PstI C_TGCA'G Cuts at: 830 3293 3572 830 Size: 2463 279 3478 Fragments arranged by size: 3478 2463 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2230 2394 2695 2923 3288 2230 Size: 164 301 228 365 5162 Fragments arranged by size: 5162 365 301 228 164 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SapI GCTCTTCn'nnn_ Cuts at: 3308 3308 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 3289 3361 3376 3568 4703 5384 5575 Size: 219 2244 72 15 192 1135 681 191 Cuts at: 5575 826 Size: 1471 Fragments arranged by size: 2244 1471 1135 681 219 192 191 72 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 2052 2382 2878 3547 1872 Size: 180 330 496 669 4545 Fragments arranged by size: 4545 669 496 330 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 2417 3440 4324 5225 5487 820 Size: 197 926 474 1023 884 901 262 1553 Fragments arranged by size: 1553 1023 926 901 884 474 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 3659 513 Size: 552 2594 3074 Fragments arranged by size: 3074 2594 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3370 4247 4264 4417 4602 5047 325 Size: 1255 1790 877 17 153 185 445 1498 Fragments arranged by size: 1790 1498 1255 877 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2791 3019 4466 Size: 274 80 33 51 1443 814 228 1447 Cuts at: 4466 6178 96 Size: 1712 138 Fragments arranged by size: 1712 1447 1443 814 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2938 2970 6180 6212 2938 Size: 32 3210 32 2946 Fragments arranged by size: 3210 2946 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2426 3389 1316 Size: 1110 963 4147 Fragments arranged by size: 4147 1110 963 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2851 3453 2011 Size: 840 602 4778 Fragments arranged by size: 4778 840 602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 2050 2380 2876 3545 1870 Size: 180 330 496 669 4545 Fragments arranged by size: 4545 669 496 330 180 XmnI GAAnn'nnTTC Cuts at: 3479 4349 3479 Size: 870 5350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI