(Circular) (Six-base) MAPSORT of: pFN21AE3341.seq Check: 6972 from: 1 to: 7663 pFN21AE3341 7663 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5473 278 Size: 53 83 186 936 3937 2468 Fragments arranged by size: 3937 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 5006 1937 Size: 142 2927 4594 Fragments arranged by size: 4594 2927 142 Acc65I G'GTAC_C Cuts at: 2767 4984 2767 Size: 2217 5446 AclI AA'CG_TT Cuts at: 1563 5790 6163 1563 Size: 4227 373 3063 Fragments arranged by size: 4227 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3559 5726 7110 7378 1632 Size: 1927 2167 1384 268 1917 Fragments arranged by size: 2167 1927 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7466 AflIII A'CryG_T Cuts at: 1203 4632 6562 7539 1203 Size: 3429 1930 977 1327 Fragments arranged by size: 3429 1930 1327 977 AgeI A'CCGG_T Cuts at: 4782 6494 4782 Size: 1712 5951 AhdI GACnn_n'nnGTC Cuts at: 6392 6392 Size: 7663 AleI CACnn'nnGTG Cuts at: 1166 2471 1166 Size: 1305 6358 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3692 1184 Size: 2508 5155 AlwNI CAG_nnn'CTG Cuts at: 2666 3296 3560 4808 5406 6978 2666 Size: 630 264 1248 598 1572 3351 Fragments arranged by size: 3351 1572 1248 630 598 264 ApaLI G'TGCA_C Cuts at: 2923 4835 5720 6876 2923 Size: 1912 885 1156 3710 Fragments arranged by size: 3710 1912 1156 885 ApoI r'AATT_y Cuts at: 1493 2309 3896 4403 4613 4730 4972 5198 5350 Size: 816 1587 507 210 117 242 226 152 Cuts at: 5350 7459 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1587 816 507 242 226 210 152 117 AseI AT'TA_AT Cuts at: 160 1032 6217 160 Size: 872 5185 1606 Fragments arranged by size: 5185 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7663 AvaI C'yCGr_G Cuts at: 1870 1943 2274 3664 4308 4988 1870 Size: 73 331 1390 644 680 4545 Fragments arranged by size: 4545 1390 680 644 331 73 BamHI G'GATC_C Cuts at: 4714 4993 5360 4714 Size: 279 367 7017 Fragments arranged by size: 7017 367 279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2767 4984 6439 618 Size: 325 217 627 980 2217 1455 1842 Fragments arranged by size: 2217 1842 1455 980 627 325 217 BanII G_rGCy'C Cuts at: 1391 2287 3585 4691 4982 1391 Size: 896 1298 1106 291 4072 Fragments arranged by size: 4072 1298 1106 896 291 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2327 2667 3191 928 Size: 971 428 340 524 5400 Fragments arranged by size: 5400 971 524 428 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5854 5888 1903 Size: 34 3917 34 3678 Fragments arranged by size: 3917 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5555 6765 5555 Size: 1210 6453 BclI T'GATC_A Cuts at: 1548 4092 1548 Size: 2544 5119 BfrBI ATG'CAT Cuts at: 4130 4130 Size: 7663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6274 136 Size: 107 122 71 631 605 4602 1525 Fragments arranged by size: 4602 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2114 3945 7658 2114 Size: 1831 3713 2119 Fragments arranged by size: 3713 2119 1831 BlpI GC'TnA_GC Cuts at: 5080 5080 Size: 7663 Bme1580I G_kGCm'C Cuts at: 1790 2885 2927 4839 5724 6880 1790 Size: 1095 42 1912 885 1156 2573 Fragments arranged by size: 2573 1912 1156 1095 885 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7663 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2635 5394 6352 Size: 416 53 83 399 24 1218 2759 958 Cuts at: 6352 442 Size: 1753 Fragments arranged by size: 2759 1753 1218 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7663 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3072 4135 3072 Size: 1063 6600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4675 4748 6323 1239 Size: 3436 73 1575 2579 Fragments arranged by size: 3436 2579 1575 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2338 4337 5788 6653 6951 2338 Size: 1999 1451 865 298 3050 Fragments arranged by size: 3050 1999 1451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3661 6326 882 Size: 617 2162 2665 2219 Fragments arranged by size: 2665 2219 2162 617 BsaAI yAC'GTr Cuts at: 493 1204 3541 3807 4840 5454 493 Size: 711 2337 266 1033 614 2702 Fragments arranged by size: 2702 2337 1033 711 614 266 BsaBI GATnn'nnATC Cuts at: 4364 7657 4364 Size: 3293 4370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2343 3919 5470 5852 Size: 53 83 186 936 810 1576 1551 382 Cuts at: 5852 6487 275 Size: 635 1451 Fragments arranged by size: 1576 1551 1451 936 810 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4327 4368 4782 6096 6494 6768 6915 7326 4327 Size: 41 414 1314 398 274 147 411 4664 Fragments arranged by size: 4664 1314 414 411 398 274 147 41 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3709 3739 1178 Size: 30 165 30 2306 30 5102 Fragments arranged by size: 5102 2306 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 4009 4612 1186 Size: 1633 1190 603 4237 Fragments arranged by size: 4237 1633 1190 603 BseYI C'CCAG_C Cuts at: 2009 2237 2795 3125 6866 7362 2009 Size: 228 558 330 3741 496 2310 Fragments arranged by size: 3741 2310 558 496 330 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2962 1163 Size: 573 1226 5864 Fragments arranged by size: 5864 1226 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5113 5874 6902 1540 Size: 275 186 3112 761 1028 2301 Fragments arranged by size: 3112 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2927 4691 4839 5724 5809 6880 2927 Size: 1764 148 885 85 1071 3710 Fragments arranged by size: 3710 1764 1071 885 148 85 BsiWI C'GTAC_G Cuts at: 3807 4461 3807 Size: 654 7009 BsmI GAATG_Cn' Cuts at: 2825 4130 5174 5267 2825 Size: 1305 1044 93 5221 Fragments arranged by size: 5221 1305 1044 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2357 3712 3926 2357 Size: 1355 214 6094 Fragments arranged by size: 6094 1355 214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 2885 2927 3585 4691 4839 4982 Size: 399 497 598 42 658 1106 148 143 Cuts at: 4982 5724 5809 6880 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1106 1071 742 658 598 497 399 148 143 85 42 BspEI T'CCGG_A Cuts at: 4327 7326 4327 Size: 2999 4664 BspHI T'CATG_A Cuts at: 2493 5552 7282 2493 Size: 3059 1730 2874 Fragments arranged by size: 3059 2874 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5018 844 Size: 4174 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2809 2814 5550 1394 Size: 429 986 5 2736 3507 Fragments arranged by size: 3507 2736 986 429 5 BsrDI GCAATG_nn' Cuts at: 65 1248 2230 2445 3333 3693 4554 4923 6158 Size: 1183 982 215 888 360 861 369 1235 Cuts at: 6158 6332 65 Size: 174 1396 Fragments arranged by size: 1396 1235 1183 982 888 861 369 360 215 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4782 6307 6494 1700 Size: 110 2972 1525 187 2869 Fragments arranged by size: 2972 2869 1525 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7663 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7663 BssSI C'ACGA_G Cuts at: 2920 5723 6735 2920 Size: 2803 1012 3848 Fragments arranged by size: 3848 2803 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4939 794 Size: 4145 3518 BstBI TT'CG_AA Cuts at: 6467 6467 Size: 7663 BstEII G'GTnAC_C Cuts at: 1184 3119 4530 1184 Size: 1935 1411 4317 Fragments arranged by size: 4317 1935 1411 BstXI CCAn_nnnn'nTGG Cuts at: 3340 4024 4777 3340 Size: 684 753 6226 Fragments arranged by size: 6226 753 684 BstYI r'GATC_y Cuts at: 1972 2114 2511 3814 3945 4714 4993 5360 5745 Size: 142 397 1303 131 769 279 367 385 Cuts at: 5745 5762 7214 7300 7312 7658 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1303 769 397 385 367 346 279 142 131 86 17 12 BstZ17I GTA'TAC Cuts at: 2080 2080 Size: 7663 Bsu36I CC'TnA_GG Cuts at: 4136 4136 Size: 7663 BtgI C'CryG_G Cuts at: 513 1065 2004 2859 513 Size: 552 939 855 5317 Fragments arranged by size: 5317 939 855 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2563 487 Size: 683 56 1337 5587 Fragments arranged by size: 5587 1337 683 56 BtsI GCAGTG_nn' Cuts at: 3676 3834 3919 4701 4912 5179 5973 5993 3676 Size: 158 85 782 211 267 794 20 5346 Fragments arranged by size: 5346 794 782 267 211 158 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7663 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3091 3126 529 Size: 35 2527 35 5066 Fragments arranged by size: 5066 2527 35 35 DraI TTT'AAA Cuts at: 4967 4967 Size: 7663 DraIII CAC_nnn'GTG Cuts at: 2922 2922 Size: 7663 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6670 809 Size: 507 5354 1802 Fragments arranged by size: 5354 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3070 4245 5110 Size: 54 1425 50 275 18 1240 1175 865 Cuts at: 5110 5999 7628 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1240 1175 889 865 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5110 1537 Size: 275 3298 4090 Fragments arranged by size: 4090 3298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2712 3753 4664 5593 1353 Size: 695 664 1041 911 929 3423 Fragments arranged by size: 3423 1041 929 911 695 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6250 6624 6770 387 Size: 1064 138 4661 374 146 1280 Fragments arranged by size: 4661 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4689 4689 Size: 7663 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3559 4675 4748 5726 6323 7110 7378 Size: 393 1927 1116 73 978 597 787 268 Cuts at: 7378 1239 Size: 1524 Fragments arranged by size: 1927 1524 1116 978 787 597 393 268 73 EcoNI CCTnn'n_nnAGG Cuts at: 2154 4010 2154 Size: 1856 5807 EcoO109I rG'GnC_Cy Cuts at: 2571 4473 4852 2571 Size: 1902 379 5382 Fragments arranged by size: 5382 1902 379 EcoRI G'AATT_C Cuts at: 4730 4972 5350 4730 Size: 242 378 7043 Fragments arranged by size: 7043 378 242 EcoRV GAT'ATC Cuts at: 3502 3502 Size: 7663 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4813 4813 Size: 7663 FspI TGC'GCA Cuts at: 6169 6169 Size: 7663 HaeII r_GCGC'y Cuts at: 1678 6810 1678 Size: 5132 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 2951 2983 4146 Size: 32 135 32 527 32 820 32 1163 Cuts at: 4146 4178 4706 4738 6304 6336 6378 6410 1373 Size: 32 528 32 1566 32 42 32 2626 Fragments arranged by size: 2626 1566 1163 820 528 527 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2707 3022 4630 5007 5253 669 Size: 988 281 769 315 1608 377 246 3079 Fragments arranged by size: 3079 1608 988 769 377 315 281 246 HindIII A'AGCT_T Cuts at: 748 2826 748 Size: 2078 5585 HpaI GTT'AAC Cuts at: 5253 5253 Size: 7663 KpnI G_GTAC'C Cuts at: 2771 4988 2771 Size: 2217 5446 MfeI C'AATT_G Cuts at: 4389 5262 4389 Size: 873 6790 MluI A'CGCG_T Cuts at: 7539 7539 Size: 7663 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4617 4692 6777 6961 1305 Size: 3312 75 2085 184 2007 Fragments arranged by size: 3312 2085 2007 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5841 Fragments arranged by size: 5841 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2471 4262 4775 5621 5980 6139 518 Size: 648 1305 1791 513 846 359 159 2042 Fragments arranged by size: 2042 1791 1305 846 648 513 359 159 MspA1I CmG'CkG Cuts at: 1606 3485 3560 4171 4570 4805 5079 5756 6904 Size: 1879 75 611 399 235 274 677 1148 Cuts at: 6904 7149 1606 Size: 245 2120 Fragments arranged by size: 2120 1879 1148 677 611 399 274 245 235 75 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6172 Fragments arranged by size: 6172 939 552 NdeI CA'TA_TG Cuts at: 387 3790 387 Size: 3403 4260 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7663 NotI GC'GGCC_GC Cuts at: 5110 5110 Size: 7663 NsiI A_TGCA'T Cuts at: 4132 4132 Size: 7663 NspI r_CATG'y Cuts at: 1132 1229 4183 4306 4636 5021 6566 1132 Size: 97 2954 123 330 385 1545 2229 Fragments arranged by size: 2954 2229 1545 385 330 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7663 PciI A'CATG_T Cuts at: 4632 6562 4632 Size: 1930 5733 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7663 PfoI T'CCnGG_A Cuts at: 2514 3417 3614 2514 Size: 903 197 6563 Fragments arranged by size: 6563 903 197 PmeI GTTT'AAAC Cuts at: 4967 4967 Size: 7663 PmlI CAC'GTG Cuts at: 3541 4840 3541 Size: 1299 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5732 5764 7245 7277 5732 Size: 32 1481 32 6118 Fragments arranged by size: 6118 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4852 4852 Size: 7663 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7663 PsiI TTA'TAA Cuts at: 5233 5233 Size: 7663 PstI C_TGCA'G Cuts at: 830 5015 830 Size: 4185 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7663 PvuII CAG'CTG Cuts at: 3485 3560 4171 4570 4805 3485 Size: 75 611 399 235 6343 Fragments arranged by size: 6343 611 399 235 75 SacI G_AGCT'C Cuts at: 4691 4691 Size: 7663 SalI G'TCGA_C Cuts at: 1936 5005 1936 Size: 3069 4594 SbfI CC_TGCA'GG Cuts at: 5015 5015 Size: 7663 ScaI AGT'ACT Cuts at: 5911 7623 5911 Size: 1712 5951 SfcI C'TryA_G Cuts at: 826 1045 5011 6146 6827 7018 826 Size: 219 3966 1135 681 191 1471 Fragments arranged by size: 3966 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7663 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7663 SmaI CCC'GGG Cuts at: 1872 4990 1872 Size: 3118 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2317 4316 5767 6668 6930 820 Size: 197 926 374 1999 1451 901 262 1553 Fragments arranged by size: 1999 1553 1451 926 901 374 262 197 SnaBI TAC'GTA Cuts at: 493 3807 5454 493 Size: 3314 1647 2702 Fragments arranged by size: 3314 2702 1647 SpeI A'CTAG_T Cuts at: 152 152 Size: 7663 SphI G_CATG'C Cuts at: 1132 4183 5021 1132 Size: 3051 838 3774 Fragments arranged by size: 3774 3051 838 SspI AAT'ATT Cuts at: 5 52 5587 5 Size: 47 5535 2081 Fragments arranged by size: 5535 2081 47 StuI AGG'CCT Cuts at: 1517 3510 4609 1517 Size: 1993 1099 4571 Fragments arranged by size: 4571 1993 1099 StyI C'CwwG_G Cuts at: 513 1065 2004 3585 4122 5102 513 Size: 552 939 1581 537 980 3074 Fragments arranged by size: 3074 1581 980 939 552 537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2124 2483 2949 5690 5707 5860 6045 Size: 1255 544 359 466 2741 17 153 185 Cuts at: 6045 6490 325 Size: 445 1498 Fragments arranged by size: 2741 1498 1255 544 466 445 359 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 2739 4104 Size: 274 80 33 51 1443 399 363 1365 Cuts at: 4104 5909 7621 96 Size: 1805 1712 138 Fragments arranged by size: 1805 1712 1443 1365 399 363 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2147 2179 2610 2642 4242 4274 7623 7655 2147 Size: 32 431 32 1600 32 3349 32 2155 Fragments arranged by size: 3349 2155 1600 431 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2060 1316 Size: 744 6919 XbaI T'CTAG_A Cuts at: 4999 4999 Size: 7663 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2477 3649 2477 Size: 1172 6491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7663 XmaI C'CCGG_G Cuts at: 1870 4988 1870 Size: 3118 4545 XmnI GAAnn'nnTTC Cuts at: 5792 5792 Size: 7663 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5471 276 Size: 53 83 186 936 3937 2468 Fragments arranged by size: 3937 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI