################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI ApoI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BciVI BclI BfrI BlnI BsaBI BsaI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV FbaI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflIII 995 ACATGT A^C[GA][CT]GT Aor51HI 2186 AGCGCT AGC^GCT AscI 174 GGCGCGCC GG^CGCGCC AspI 805 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1705 CACGTG CAC^GTG BbsI 1684 GTCTTC GAAGAC(2/6) BbvCI 645 CCTCAGC CCTCAGC(-5/-2) BcgI 800 GCAACGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1484 AGATCT A^GATCT BpuAI 1684 GTCTTC GAAGAC(2/6) BsmBI 1519 CGTCTC CGTCTC(1/5) BsmI 845 GCATTC GAATGC(1/-1) BspLU11I 995 ACATGT A^CATGT BssSI 883 CACGAG CACGAG(-5/-1) BstEII 877 GGTGACC G^GT[ACGT]ACC BstPI 877 GGTGACC G^GT[ACGT]ACC EarI 2092 GAAGAG CTCTTC(1/4) Eco47III 2186 AGCGCT AGC^GCT EcoO65I 877 GGTGACC G^GT[ACGT]ACC EcoT14I 1747 CCATGG C^C[AT][AT]GG FseI 679 GGCCGGCC GGCCGG^CC HindIII 1013 AAGCTT A^AGCTT HinfI 139 GACTC G^A[ACGT]TC Ksp632I 2092 GAAGAG CTCTTC(1/4) NcoI 1747 CCATGG C^CATGG PflFI 805 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 139 GACTC GAGTC(4/5) PmaCI 1705 CACGTG CAC^GTG PmlI 1705 CACGTG CAC^GTG SfiI 32 GGCCCGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 2257 CTTGAG C^T[CT][GA]AG StyI 1747 CCATGG C^C[AT][AT]GG TaqI 851 TCGA T^CGA Tsp509I 247 AATT ^AATT Tth111I 805 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 851 TCGA T^CGA XbaI 1287 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 235 GGCGCC G[GA]^CG[CT]C 2066 GGCGCC Alw44I 892 GTGCAC G^TGCAC 991 GTGCAC AlwNI 349 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1336 CAGCAGCTG ApaLI 892 GTGCAC G^TGCAC 991 GTGCAC BalI 639 TGGCCA TGG^CCA 2285 TGGCCA BbeI 235 GGCGCC GGCGC^C 2066 GGCGCC BfaI 1288 CTAG C^TAG 1540 CTAG BsaHI 235 GGCGCC G[GA]^CG[CT]C 2066 GGCGCC BsiEI 717 CGGCCG CG[GA][CT]^CG 1465 CGGCCG BsmAI 137 GAGAC GTCTC(1/5) 1520 GTCTC BspMI 527 ACCTGC ACCTGC(4/8) 548 ACCTGC BsrBI 203 GAGCGG CCGCTC(-3/-3) 1720 GAGCGG BssHII 175 GCGCGC G^CGCGC 817 GCGCGC BstF5I 1145 CATCC GGATG(2/0) 2115 GGATG BstXI 857 CCACCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1085 CCAGCCATCTGG DraIII 973 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1238 CACTGCGTG EagI 717 CGGCCG C^GGCCG 1465 CGGCCG EclXI 717 CGGCCG C^GGCCG 1465 CGGCCG Eco52I 717 CGGCCG C^GGCCG 1465 CGGCCG EcoT22I 1888 ATGCAT ATGCA^T 1924 ATGCAT FokI 1145 CATCC GGATG(9/13) 2115 GGATG Hin1I 235 GGCGCC G[GA]^CG[CT]C 2066 GGCGCC KasI 235 GGCGCC G^GCGCC 2066 GGCGCC MaeI 1288 CTAG C^TAG 1540 CTAG MluNI 639 TGGCCA TGG^CCA 2285 TGGCCA MscI 639 TGGCCA TGG^CCA 2285 TGGCCA NarI 235 GGCGCC GG^CGCC 2066 GGCGCC NsiI 1888 ATGCAT ATGCA^T 1924 ATGCAT PstI 1592 CTGCAG CTGCA^G 2182 CTGCAG SfoI 235 GGCGCC GGC^GCC 2066 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SphI StuI TseI Tsp45I TspRI Van91I XcmI XhoII XmaCI XmaI