Restriction map for
pFN21AE2573
(Circular) (Six-base) MAPSORT of: pFN21AE2573.seq Check: 9692 from: 1 to: 7030
pFN21AE2573 7030 bp
With 182 enzymes: SgfI *
May 7, 2015 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2541 2562 2541
Size: 21 7009
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4840 278
Size: 53 83 186 936 3304 2468
Fragments arranged by size:
3304 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4373 1937
Size: 2436 4594
Acc65I G'GTAC_C
Cuts at: 4351 4351
Size: 7030
AclI AA'CG_TT
Cuts at: 1563 5157 5530 1563
Size: 3594 373 3063
Fragments arranged by size:
3594 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5093 6477 6745 1632
Size: 3461 1384 268 1917
Fragments arranged by size:
3461 1917 1384 268
AfeI AGC'GCT
Cuts at: 4193 4193
Size: 7030
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6833
AflIII A'CryG_T
Cuts at: 1203 3000 5929 6906 1203
Size: 1797 2929 977 1327
Fragments arranged by size:
2929 1797 1327 977
AgeI A'CCGG_T
Cuts at: 5861 5861
Size: 7030
AhdI GACnn_n'nnGTC
Cuts at: 5759 5759
Size: 7030
AleI CACnn'nnGTG
Cuts at: 1166 2102 2867 1166
Size: 936 765 5329
Fragments arranged by size:
5329 936 765
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2786 1184
Size: 1602 5428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 6998
AlwNI CAG_nnn'CTG
Cuts at: 2359 3346 4773 6345 2359
Size: 987 1427 1572 3044
Fragments arranged by size:
3044 1572 1427 987
ApaI G_GGCC'C
Cuts at: 2049 2482 3981 4204 2049
Size: 433 1499 223 4875
Fragments arranged by size:
4875 1499 433 223
ApaLI G'TGCA_C
Cuts at: 2897 2996 5087 6243 2897
Size: 99 2091 1156 3684
Fragments arranged by size:
3684 2091 1156 99
ApoI r'AATT_y
Cuts at: 1493 4339 4565 4717 6826 1493
Size: 2846 226 152 2109 1697
Fragments arranged by size:
2846 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2180 2180
Size: 7030
AseI AT'TA_AT
Cuts at: 160 1032 5584 160
Size: 872 4552 1606
Fragments arranged by size:
4552 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7030
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 3027 3378 3744 4355 1870
Size: 73 282 802 351 366 611 4545
Fragments arranged by size:
4545 802 611 366 351 282 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3032 3065 3032
Size: 33 6997
BamHI G'GATC_C
Cuts at: 4360 4727 4360
Size: 367 6663
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 2430 2596 3740 3748
Size: 325 217 627 453 190 166 1144 8
Cuts at: 3748 4071 4351 5806 618
Size: 323 280 1455 1842
Fragments arranged by size:
1842 1455 1144 627 453 325 323 280
217 190 166 8
BanII G_rGCy'C
Cuts at: 1391 2049 2482 2715 2924 3981 4204 4349 1391
Size: 658 433 233 209 1057 223 145 4072
Fragments arranged by size:
4072 1057 658 433 233 223 209 145
BbeI G_GCGC'C
Cuts at: 2244 4075 2244
Size: 1831 5199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3682 928
Size: 971 1783 4276
Fragments arranged by size:
4276 1783 971
BbvCI CC'TCA_GC
Cuts at: 2651 2651
Size: 7030
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2794 2828 5221 5255 1903
Size: 34 50 34 773 34 2393 34 3678
Fragments arranged by size:
3678 2393 773 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4922 6132 4922
Size: 1210 5820
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7030
BfrBI ATG'CAT
Cuts at: 3895 3931 3895
Size: 36 6994
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2044 2309
Size: 107 122 71 631 605 337 35 265
Cuts at: 2309 2643 2739 3713 4031 5641 136
Size: 334 96 974 318 1610 1525
Fragments arranged by size:
1610 1525 974 631 605 337 334 318
265 122 107 96 71 35
BglII A'GATC_T
Cuts at: 3489 7025 3489
Size: 3536 3494
BlpI GC'TnA_GC
Cuts at: 2090 2918 3476 3555 4447 2090
Size: 828 558 79 892 4673
Fragments arranged by size:
4673 892 828 558 79
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2433 2482 2901 2968 3000 3504 3745
Size: 259 384 49 419 67 32 504 241
Cuts at: 3745 3981 4204 5091 6247 1790
Size: 236 223 887 1156 2573
Fragments arranged by size:
2573 1156 887 504 419 384 259 241
236 223 67 49 32
BmgBI CAC'GTC
Cuts at: 1318 3524 4209 1318
Size: 2206 685 4139
Fragments arranged by size:
4139 2206 685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4761 5719 442
Size: 416 53 83 399 24 3344 958 1753
Fragments arranged by size:
3344 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 2459 2579 2933 3317 3431 3626 3743
Size: 921 299 120 354 384 114 195 117
Cuts at: 3743 4130 4132 4235 5690 1239
Size: 387 2 103 1455 2579
Fragments arranged by size:
2579 1455 921 387 384 354 299 195
120 117 114 103 2
Bpu10I CC'TnA_GC
Cuts at: 2081 2651 2081
Size: 570 6460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4283 5155 6020 6318 4283
Size: 872 865 298 4995
Fragments arranged by size:
4995 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5693 882
Size: 617 4194 2219
Fragments arranged by size:
4194 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3048 3712 4057 4821 493
Size: 711 1844 664 345 764 2702
Fragments arranged by size:
2702 1844 764 711 664 345
BsaBI GATnn'nnATC
Cuts at: 7024 7024
Size: 7030
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 4072 4837 5219
Size: 53 83 186 936 708 1831 765 382
Cuts at: 5219 5854 275
Size: 635 1451
Fragments arranged by size:
1831 1451 936 765 708 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5463 5861 6135 6282 6693 5463
Size: 398 274 147 411 5800
Fragments arranged by size:
5800 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 3056 3086 3197
Size: 30 165 30 696 30 927 30 111
Cuts at: 3197 3227 1178
Size: 30 4981
Fragments arranged by size:
4981 927 696 165 111 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2528 3311 4115 1186
Size: 937 405 783 804 4101
Fragments arranged by size:
4101 937 804 783 405
BseYI C'CCAG_C
Cuts at: 2341 2401 2425 2506 2839 3183 3666 3949 3988
Size: 60 24 81 333 344 483 283 39
Cuts at: 3988 4284 6233 6729 2341
Size: 296 1949 496 2642
Fragments arranged by size:
2642 1949 496 483 344 333 296 283
81 60 39 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2268 2519 2860 2957 2996 3479
Size: 573 483 49 251 341 97 39 483
Cuts at: 3479 3785 4231 1163
Size: 306 446 3962
Fragments arranged by size:
3962 573 483 483 446 341 306 251
97 49 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 3473 4480 5241 6269 1540
Size: 275 186 724 748 1007 761 1028 2301
Fragments arranged by size:
2301 1028 1007 761 748 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2901 3000 3864 5091 5176 6247 2901
Size: 99 864 1227 85 1071 3684
Fragments arranged by size:
3684 1227 1071 864 99 85
BsmI GAATG_Cn'
Cuts at: 2850 4541 4634 2850
Size: 1691 93 5246
Fragments arranged by size:
5246 1691 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3530 3530
Size: 7030
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2433 2482 2715 2901 2924 2968
Size: 399 259 384 49 233 186 23 44
Cuts at: 2968 3000 3504 3745 3864 3981 4204 4349 5091
Size: 32 504 241 119 117 223 145 742
Cuts at: 5091 5176 6247 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 384 259 241
233 223 186 145 119 117 85 49
44 32 23
BspEI T'CCGG_A
Cuts at: 6693 6693
Size: 7030
BspHI T'CATG_A
Cuts at: 4919 6649 4919
Size: 1730 5300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 2562 4385 844
Size: 1697 21 1823 3489
Fragments arranged by size:
3489 1823 1697 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2210 3727 4917 1394
Size: 429 387 1517 1190 3507
Fragments arranged by size:
3507 1517 1190 429 387
BsrDI GCAATG_nn'
Cuts at: 65 1248 5525 5699 65
Size: 1183 4277 174 1396
Fragments arranged by size:
4277 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 2231 2433 2685 3438 4177 5674
Size: 110 374 47 202 252 753 739 1497
Cuts at: 5674 5861 1700
Size: 187 2869
Fragments arranged by size:
2869 1497 753 739 374 252 202 187
110 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7030
BssHII G'CGCG_C
Cuts at: 1925 2180 2822 1925
Size: 255 642 6133
Fragments arranged by size:
6133 642 255
BssSI C'ACGA_G
Cuts at: 2888 5090 6102 2888
Size: 2202 1012 3816
Fragments arranged by size:
3816 2202 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2359 2779 4162 794
Size: 1565 420 1383 3662
Fragments arranged by size:
3662 1565 1383 420
BstBI TT'CG_AA
Cuts at: 5834 5834
Size: 7030
BstEII G'GTnAC_C
Cuts at: 1184 2882 1184
Size: 1698 5332
BstXI CCAn_nnnn'nTGG
Cuts at: 2869 3097 2869
Size: 228 6802
BstYI r'GATC_y
Cuts at: 1972 3057 3078 3489 3799 4360 4727 5112 5129
Size: 1085 21 411 310 561 367 385 17
Cuts at: 5129 6581 6667 6679 7025 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1085 561 411 385 367 346
310 86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2049 2055 2734 3132 3752 4066 513
Size: 552 984 6 679 398 620 314 3477
Fragments arranged by size:
3477 984 679 620 552 398 314 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1998 487
Size: 683 56 772 5519
Fragments arranged by size:
5519 772 683 56
BtsI GCAGTG_nn'
Cuts at: 2502 3242 3594 3785 4546 5340 5360 2502
Size: 740 352 191 761 794 20 4172
Fragments arranged by size:
4172 794 761 740 352 191 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7030
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6995
DraI TTT'AAA
Cuts at: 4334 4334
Size: 7030
DraIII CAC_nnn'GTG
Cuts at: 2983 3248 2983
Size: 265 6765
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6037 809
Size: 507 4721 1802
Fragments arranged by size:
4721 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2293 2409
Size: 54 1425 50 275 18 222 241 116
Cuts at: 2409 2644 2722 3099 3270 3470 3843 4003 4290
Size: 235 78 377 171 200 373 160 287
Cuts at: 4290 4477 5366 6995 8
Size: 187 889 1629 43
Fragments arranged by size:
1629 1425 889 377 373 287 275 241
235 222 200 187 171 160 116 78
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 3470 4477 1537
Size: 275 910 748 1007 4090
Fragments arranged by size:
4090 1007 910 748 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4092 4960 1353
Size: 2739 868 3423
Fragments arranged by size:
3423 2739 868
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2653 2721 5617 5991 6137 387
Size: 1064 138 1064 68 2896 374 146 1280
Fragments arranged by size:
2896 1280 1064 1064 374 146 138 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2459 2579 2933 3317 3431 3626
Size: 393 528 299 120 354 384 114 195
Cuts at: 3626 3743 4130 4132 4235 5093 5690 6477 6745
Size: 117 387 2 103 858 597 787 268
Cuts at: 6745 1239
Size: 1524
Fragments arranged by size:
1524 858 787 597 528 393 387 384
354 299 268 195 120 117 114 103
2
EcoO109I rG'GnC_Cy
Cuts at: 2045 2319 2479 2947 3971 3978 4231 4267 2045
Size: 274 160 468 1024 7 253 36 4808
Fragments arranged by size:
4808 1024 468 274 253 160 36 7
EcoRI G'AATT_C
Cuts at: 4339 4717 4339
Size: 378 6652
FseI GG_CCGG'CC
Cuts at: 2689 2689
Size: 7030
FspI TGC'GCA
Cuts at: 5536 5536
Size: 7030
HaeII r_GCGC'y
Cuts at: 1678 2244 2546 4075 4184 4195 6177 1678
Size: 566 302 1529 109 11 1982 2531
Fragments arranged by size:
2531 1982 1529 566 302 109 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3791 3823 5671 5703 5745
Size: 32 135 32 2219 32 1848 32 42
Cuts at: 5745 5777 1373
Size: 32 2626
Fragments arranged by size:
2626 2219 1848 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4374 4620 669
Size: 988 281 2436 246 3079
Fragments arranged by size:
3079 2436 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3018 748
Size: 2270 4760
HpaI GTT'AAC
Cuts at: 4620 4620
Size: 7030
KasI G'GCGC_C
Cuts at: 2240 4071 2240
Size: 1831 5199
KpnI G_GTAC'C
Cuts at: 4355 4355
Size: 7030
MfeI C'AATT_G
Cuts at: 4629 4629
Size: 7030
MluI A'CGCG_T
Cuts at: 6906 6906
Size: 7030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4074 6144 6328 1305
Size: 2769 2070 184 2007
Fragments arranged by size:
2769 2070 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2646 4292 10
Size: 54 1425 343 814 1646 2748
Fragments arranged by size:
2748 1646 1425 814 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2495 2867 3684 4988 5347 5506
Size: 648 936 393 372 817 1304 359 159
Cuts at: 5506 518
Size: 2042
Fragments arranged by size:
2042 1304 936 817 648 393 372 359
159
MspA1I CmG'CkG
Cuts at: 1606 3010 3122 3237 3346 3409 3463 3475 3823
Size: 1404 112 115 109 63 54 12 348
Cuts at: 3823 3885 3953 4245 4288 4446 5123 6271 6516
Size: 62 68 292 43 158 677 1148 245
Cuts at: 6516 1606
Size: 2120
Fragments arranged by size:
2120 1404 1148 677 348 292 245 158
115 112 109 68 63 62 54 43
12
NaeI GCC'GGC
Cuts at: 2186 2233 2687 3440 4179 2186
Size: 47 454 753 739 5037
Fragments arranged by size:
5037 753 739 454 47
NarI GG'CG_CC
Cuts at: 2241 4072 2241
Size: 1831 5199
NcoI C'CATG_G
Cuts at: 513 1065 3752 513
Size: 552 2687 3791
Fragments arranged by size:
3791 2687 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7030
NgoMIV G'CCGG_C
Cuts at: 2184 2231 2685 3438 4177 2184
Size: 47 454 753 739 5037
Fragments arranged by size:
5037 753 739 454 47
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7030
NotI GC'GGCC_GC
Cuts at: 4477 4477
Size: 7030
NsiI A_TGCA'T
Cuts at: 3897 3933 3897
Size: 36 6994
NspI r_CATG'y
Cuts at: 1132 1229 2767 3004 3895 3899 4160 4388 5933
Size: 97 1538 237 891 4 261 228 1545
Cuts at: 5933 1132
Size: 2229
Fragments arranged by size:
2229 1545 1538 891 261 237 228 97
4
PasI CC'CwG_GG
Cuts at: 1348 2068 2924 3427 1348
Size: 720 856 503 4951
Fragments arranged by size:
4951 856 720 503
PciI A'CATG_T
Cuts at: 3000 5929 3000
Size: 2929 4101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 3248 3673 1108
Size: 1449 691 425 4465
Fragments arranged by size:
4465 1449 691 425
PfoI T'CCnGG_A
Cuts at: 3060 3060
Size: 7030
PmeI GTTT'AAAC
Cuts at: 4334 4334
Size: 7030
PmlI CAC'GTG
Cuts at: 3712 3712
Size: 7030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5099 5131 6612 6644 5099
Size: 32 1481 32 5485
Fragments arranged by size:
5485 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2319 2947 4231 2319
Size: 628 1284 5118
Fragments arranged by size:
5118 1284 628
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7030
PsiI TTA'TAA
Cuts at: 4600 4600
Size: 7030
PspOMI G'GGCC_C
Cuts at: 2045 2478 3977 4200 2045
Size: 433 1499 223 4875
Fragments arranged by size:
4875 1499 433 223
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3296 3328 4118 4150 3296
Size: 32 790 32 6176
Fragments arranged by size:
6176 790 32 32
PstI C_TGCA'G
Cuts at: 830 3601 4191 4382 830
Size: 2771 590 191 3478
Fragments arranged by size:
3478 2771 590 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7030
PvuII CAG'CTG
Cuts at: 3010 3122 3346 3409 3463 3953 4288 3010
Size: 112 224 63 54 490 335 5752
Fragments arranged by size:
5752 490 335 224 112 63 54
SalI G'TCGA_C
Cuts at: 1936 4372 1936
Size: 2436 4594
SanDI GG'GwC_CC
Cuts at: 2947 2947
Size: 7030
SbfI CC_TGCA'GG
Cuts at: 4382 4382
Size: 7030
ScaI AGT'ACT
Cuts at: 5278 6990 5278
Size: 1712 5318
SfcI C'TryA_G
Cuts at: 826 1045 2902 3597 4187 4378 5513 6194 6385
Size: 219 1857 695 590 191 1135 681 191
Cuts at: 6385 826
Size: 1471
Fragments arranged by size:
1857 1471 1135 695 681 590 219 191
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2044 2044
Size: 7030
SfoI GGC'GCC
Cuts at: 2242 4073 2242
Size: 1831 5199
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7030
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7030
SmaI CCC'GGG
Cuts at: 1872 2227 3380 3746 4357 1872
Size: 355 1153 366 611 4545
Fragments arranged by size:
4545 1153 611 366 355
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4262 5134 6035 6297 820
Size: 197 926 2319 872 901 262 1553
Fragments arranged by size:
2319 1553 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 4821 493
Size: 4328 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7030
SphI G_CATG'C
Cuts at: 1132 2767 3895 3899 4388 1132
Size: 1635 1128 4 489 3774
Fragments arranged by size:
3774 1635 1128 489 4
SspI AAT'ATT
Cuts at: 5 52 4954 5
Size: 47 4902 2081
Fragments arranged by size:
4902 2081 47
StuI AGG'CCT
Cuts at: 1517 3518 3587 3917 4034 1517
Size: 2001 69 330 117 4513
Fragments arranged by size:
4513 2001 330 117 69
StyI C'CwwG_G
Cuts at: 513 1065 3752 4469 513
Size: 552 2687 717 3074
Fragments arranged by size:
3074 2687 717 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3963 5057 5074 5227 5412 5857
Size: 1255 512 1871 1094 17 153 185 445
Cuts at: 5857 325
Size: 1498
Fragments arranged by size:
1871 1498 1255 1094 512 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5276 6988 96
Size: 274 80 33 51 1443 3299 1712 138
Fragments arranged by size:
3299 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 3056 3088 3197 3229 6990 7022 2099
Size: 32 925 32 109 32 3761 32 2107
Fragments arranged by size:
3761 2107 925 109 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2813 1316
Size: 1497 5533
XbaI T'CTAG_A
Cuts at: 3292 4366 3292
Size: 1074 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2404 2741 3601 2404
Size: 337 860 5833
Fragments arranged by size:
5833 860 337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7030
XmaI C'CCGG_G
Cuts at: 1870 2225 3378 3744 4355 1870
Size: 355 1153 366 611 4545
Fragments arranged by size:
4545 1153 611 366 355
XmnI GAAnn'nnTTC
Cuts at: 5159 5159
Size: 7030
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4838 276
Size: 53 83 186 936 3304 2468
Fragments arranged by size:
3304 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FspAI NruI PacI PspXI RsrII SacI SacII
SapI SexAI SrfI SwaI