(Linear) (Six-base) MAPSORT of: KIEE2573.seq Check: 8966 from: 1 to: 2325 KIEE2573.orf With 182 enzymes: SgfI * May 7, 2015 14:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 536 557 2325 Size: 536 21 1768 Fragments arranged by size: 1768 536 21 AfeI AGC'GCT Cuts at: 0 2188 2325 Size: 2188 137 AflIII A'CryG_T Cuts at: 0 995 2325 Size: 995 1330 AleI CACnn'nnGTG Cuts at: 0 97 862 2325 Size: 97 765 1463 Fragments arranged by size: 1463 765 97 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 781 2325 Size: 781 1544 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1813 1845 2325 Size: 1813 32 480 Fragments arranged by size: 1813 480 32 AlwNI CAG_nnn'CTG Cuts at: 0 354 1341 2325 Size: 354 987 984 Fragments arranged by size: 987 984 354 ApaI G_GGCC'C Cuts at: 0 44 477 1976 2199 2325 Size: 44 433 1499 223 126 Fragments arranged by size: 1499 433 223 126 44 ApaLI G'TGCA_C Cuts at: 0 892 991 2325 Size: 892 99 1334 Fragments arranged by size: 1334 892 99 AscI GG'CGCG_CC Cuts at: 0 175 2325 Size: 175 2150 AvaI C'yCGr_G Cuts at: 0 220 1022 1373 1739 2325 Size: 220 802 351 366 586 Fragments arranged by size: 802 586 366 351 220 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1027 1060 2325 Size: 1027 33 1265 Fragments arranged by size: 1265 1027 33 BanI G'GyrC_C Cuts at: 0 235 425 591 1735 1743 2066 2325 Size: 235 190 166 1144 8 323 259 Fragments arranged by size: 1144 323 259 235 190 166 8 BanII G_rGCy'C Cuts at: 0 44 477 710 919 1976 2199 2325 Size: 44 433 233 209 1057 223 126 Fragments arranged by size: 1057 433 233 223 209 126 44 BbeI G_GCGC'C Cuts at: 0 239 2070 2325 Size: 239 1831 255 Fragments arranged by size: 1831 255 239 BbsI GAAGACnn'nnnn_ Cuts at: 0 1677 2325 Size: 1677 648 BbvCI CC'TCA_GC Cuts at: 0 646 2325 Size: 646 1679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 789 823 2325 Size: 789 34 1502 Fragments arranged by size: 1502 789 34 BfrBI ATG'CAT Cuts at: 0 1890 1926 2325 Size: 1890 36 399 Fragments arranged by size: 1890 399 36 BglI GCCn_nnn'nGGC Cuts at: 0 39 304 638 734 1708 2026 2325 Size: 39 265 334 96 974 318 299 Fragments arranged by size: 974 334 318 299 265 96 39 BglII A'GATC_T Cuts at: 0 1484 2325 Size: 1484 841 BlpI GC'TnA_GC Cuts at: 0 85 913 1471 1550 2325 Size: 85 828 558 79 775 Fragments arranged by size: 828 775 558 85 79 Bme1580I G_kGCm'C Cuts at: 0 44 428 477 896 963 995 1499 1740 Size: 44 384 49 419 67 32 504 241 Cuts at: 1740 1976 2199 2325 Size: 236 223 126 Fragments arranged by size: 504 419 384 241 236 223 126 67 49 44 32 BmgBI CAC'GTC Cuts at: 0 1519 2204 2325 Size: 1519 685 121 Fragments arranged by size: 1519 685 121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 454 574 928 1312 1426 1621 1738 Size: 155 299 120 354 384 114 195 117 Cuts at: 1738 2125 2127 2230 2325 Size: 387 2 103 95 Fragments arranged by size: 387 384 354 299 195 155 120 117 114 103 95 2 Bpu10I CC'TnA_GC Cuts at: 0 76 646 2325 Size: 76 570 1679 Fragments arranged by size: 1679 570 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2278 2325 Size: 2278 47 BsaAI yAC'GTr Cuts at: 0 1043 1707 2052 2325 Size: 1043 664 345 273 Fragments arranged by size: 1043 664 345 273 BsaHI Gr'CG_yC Cuts at: 0 236 2067 2325 Size: 236 1831 258 Fragments arranged by size: 1831 258 236 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 94 124 1051 1081 1192 1222 2325 Size: 94 30 927 30 111 30 1103 Fragments arranged by size: 1103 927 111 94 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 118 523 1306 2110 2325 Size: 118 405 783 804 215 Fragments arranged by size: 804 783 405 215 118 BseYI C'CCAG_C Cuts at: 0 336 396 420 501 834 1178 1661 1944 Size: 336 60 24 81 333 344 483 283 Cuts at: 1944 1983 2279 2325 Size: 39 296 46 Fragments arranged by size: 483 344 336 333 296 283 81 60 46 39 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 263 514 855 952 991 1474 1780 Size: 214 49 251 341 97 39 483 306 Cuts at: 1780 2226 2325 Size: 446 99 Fragments arranged by size: 483 446 341 306 251 214 99 97 49 39 BsiEI CG_ry'CG Cuts at: 0 720 1468 2325 Size: 720 748 857 Fragments arranged by size: 857 748 720 BsiHKAI G_wGCw'C Cuts at: 0 896 995 1859 2325 Size: 896 99 864 466 Fragments arranged by size: 896 864 466 99 BsmI GAATG_Cn' Cuts at: 0 845 2325 Size: 845 1480 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1525 2325 Size: 1525 800 Bsp1286I G_dGCh'C Cuts at: 0 44 428 477 710 896 919 963 995 Size: 44 384 49 233 186 23 44 32 Cuts at: 995 1499 1740 1859 1976 2199 2325 Size: 504 241 119 117 223 126 Fragments arranged by size: 504 384 241 233 223 186 126 119 117 49 44 44 32 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 536 557 2325 Size: 536 21 1768 Fragments arranged by size: 1768 536 21 BsrBI CCG'CTC Cuts at: 0 205 1722 2325 Size: 205 1517 603 Fragments arranged by size: 1517 603 205 BsrFI r'CCGG_y Cuts at: 0 179 226 428 680 1433 2172 2325 Size: 179 47 202 252 753 739 153 Fragments arranged by size: 753 739 252 202 179 153 47 BssHII G'CGCG_C Cuts at: 0 175 817 2325 Size: 175 642 1508 Fragments arranged by size: 1508 642 175 BssSI C'ACGA_G Cuts at: 0 883 2325 Size: 883 1442 BstAPI GCAn_nnn'nTGC Cuts at: 0 354 774 2157 2325 Size: 354 420 1383 168 Fragments arranged by size: 1383 420 354 168 BstEII G'GTnAC_C Cuts at: 0 877 2325 Size: 877 1448 BstXI CCAn_nnnn'nTGG Cuts at: 0 864 1092 2325 Size: 864 228 1233 Fragments arranged by size: 1233 864 228 BstYI r'GATC_y Cuts at: 0 1052 1073 1484 1794 2325 Size: 1052 21 411 310 531 Fragments arranged by size: 1052 531 411 310 21 BtgI C'CryG_G Cuts at: 0 44 50 729 1127 1747 2061 2325 Size: 44 6 679 398 620 314 264 Fragments arranged by size: 679 620 398 314 264 44 6 BtsI GCAGTG_nn' Cuts at: 0 497 1237 1589 1780 2325 Size: 497 740 352 191 545 Fragments arranged by size: 740 545 497 352 191 DraIII CAC_nnn'GTG Cuts at: 0 978 1243 2325 Size: 978 265 1082 Fragments arranged by size: 1082 978 265 EaeI y'GGCC_r Cuts at: 0 47 288 404 639 717 1094 1265 1465 Size: 47 241 116 235 78 377 171 200 Cuts at: 1465 1838 1998 2285 2325 Size: 373 160 287 40 Fragments arranged by size: 377 373 287 241 235 200 171 160 116 78 47 40 EagI C'GGCC_G Cuts at: 0 717 1465 2325 Size: 717 748 860 Fragments arranged by size: 860 748 717 EarI CTCTTCn'nnn_ Cuts at: 0 2087 2325 Size: 2087 238 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 648 716 2325 Size: 648 68 1609 Fragments arranged by size: 1609 648 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 155 454 574 928 1312 1426 1621 1738 Size: 155 299 120 354 384 114 195 117 Cuts at: 1738 2125 2127 2230 2325 Size: 387 2 103 95 Fragments arranged by size: 387 384 354 299 195 155 120 117 114 103 95 2 EcoO109I rG'GnC_Cy Cuts at: 0 40 314 474 942 1966 1973 2226 2262 Size: 40 274 160 468 1024 7 253 36 Cuts at: 2262 2325 Size: 63 Fragments arranged by size: 1024 468 274 253 160 63 40 36 7 FseI GG_CCGG'CC Cuts at: 0 684 2325 Size: 684 1641 HaeII r_GCGC'y Cuts at: 0 239 541 2070 2179 2190 2325 Size: 239 302 1529 109 11 135 Fragments arranged by size: 1529 302 239 135 109 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1786 1818 2325 Size: 1786 32 507 Fragments arranged by size: 1786 507 32 HindIII A'AGCT_T Cuts at: 0 1013 2325 Size: 1013 1312 KasI G'GCGC_C Cuts at: 0 235 2066 2325 Size: 235 1831 259 Fragments arranged by size: 1831 259 235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2069 2325 Size: 2069 256 MscI TGG'CCA Cuts at: 0 641 2287 2325 Size: 641 1646 38 Fragments arranged by size: 1646 641 38 MslI CAynn'nnrTG Cuts at: 0 97 490 862 1679 2325 Size: 97 393 372 817 646 Fragments arranged by size: 817 646 393 372 97 MspA1I CmG'CkG Cuts at: 0 1005 1117 1232 1341 1404 1458 1470 1818 Size: 1005 112 115 109 63 54 12 348 Cuts at: 1818 1880 1948 2240 2283 2325 Size: 62 68 292 43 42 Fragments arranged by size: 1005 348 292 115 112 109 68 63 62 54 43 42 12 NaeI GCC'GGC Cuts at: 0 181 228 682 1435 2174 2325 Size: 181 47 454 753 739 151 Fragments arranged by size: 753 739 454 181 151 47 NarI GG'CG_CC Cuts at: 0 236 2067 2325 Size: 236 1831 258 Fragments arranged by size: 1831 258 236 NcoI C'CATG_G Cuts at: 0 1747 2325 Size: 1747 578 NgoMIV G'CCGG_C Cuts at: 0 179 226 680 1433 2172 2325 Size: 179 47 454 753 739 153 Fragments arranged by size: 753 739 454 179 153 47 NsiI A_TGCA'T Cuts at: 0 1892 1928 2325 Size: 1892 36 397 Fragments arranged by size: 1892 397 36 NspI r_CATG'y Cuts at: 0 762 999 1890 1894 2155 2325 Size: 762 237 891 4 261 170 Fragments arranged by size: 891 762 261 237 170 4 PasI CC'CwG_GG Cuts at: 0 63 919 1422 2325 Size: 63 856 503 903 Fragments arranged by size: 903 856 503 63 PciI A'CATG_T Cuts at: 0 995 2325 Size: 995 1330 PflMI CCAn_nnn'nTGG Cuts at: 0 552 1243 1668 2325 Size: 552 691 425 657 Fragments arranged by size: 691 657 552 425 PfoI T'CCnGG_A Cuts at: 0 1055 2325 Size: 1055 1270 PmlI CAC'GTG Cuts at: 0 1707 2325 Size: 1707 618 PpuMI rG'GwC_Cy Cuts at: 0 314 942 2226 2325 Size: 314 628 1284 99 Fragments arranged by size: 1284 628 314 99 PspOMI G'GGCC_C Cuts at: 0 40 473 1972 2195 2325 Size: 40 433 1499 223 130 Fragments arranged by size: 1499 433 223 130 40 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1291 1323 2113 2145 2325 Size: 1291 32 790 32 180 Fragments arranged by size: 1291 790 180 32 32 PstI C_TGCA'G Cuts at: 0 1596 2186 2325 Size: 1596 590 139 Fragments arranged by size: 1596 590 139 PvuII CAG'CTG Cuts at: 0 1005 1117 1341 1404 1458 1948 2283 2325 Size: 1005 112 224 63 54 490 335 42 Fragments arranged by size: 1005 490 335 224 112 63 54 42 SanDI GG'GwC_CC Cuts at: 0 942 2325 Size: 942 1383 SfcI C'TryA_G Cuts at: 0 897 1592 2182 2325 Size: 897 695 590 143 Fragments arranged by size: 897 695 590 143 SfiI GGCCn_nnn'nGGCC Cuts at: 0 39 2325 Size: 39 2286 SfoI GGC'GCC Cuts at: 0 237 2068 2325 Size: 237 1831 257 Fragments arranged by size: 1831 257 237 SmaI CCC'GGG Cuts at: 0 222 1375 1741 2325 Size: 222 1153 366 584 Fragments arranged by size: 1153 584 366 222 SmlI C'TyrA_G Cuts at: 0 2257 2325 Size: 2257 68 SphI G_CATG'C Cuts at: 0 762 1890 1894 2325 Size: 762 1128 4 431 Fragments arranged by size: 1128 762 431 4 StuI AGG'CCT Cuts at: 0 1513 1582 1912 2029 2325 Size: 1513 69 330 117 296 Fragments arranged by size: 1513 330 296 117 69 StyI C'CwwG_G Cuts at: 0 1747 2325 Size: 1747 578 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 87 1958 2325 Size: 87 1871 367 Fragments arranged by size: 1871 367 87 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 94 126 1051 1083 1192 1224 2325 Size: 94 32 925 32 109 32 1101 Fragments arranged by size: 1101 925 109 94 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 808 2325 Size: 808 1517 XbaI T'CTAG_A Cuts at: 0 1287 2325 Size: 1287 1038 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 399 736 1596 2325 Size: 399 337 860 729 Fragments arranged by size: 860 729 399 337 XmaI C'CCGG_G Cuts at: 0 220 1373 1739 2325 Size: 220 1153 366 586 Fragments arranged by size: 1153 586 366 220 Enzymes that do cut: AarI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI AscI AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FseI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SanDI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AhdI ApoI AseI AsiSI AvrII BamHI BciVI BclI BmrI BmtI BplI BsaI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI HincII HpaI KpnI MfeI MluI NdeI NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI XhoI XmnI ZraI