################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KspI MaeI MfeI MluI MroI MseI MunI MvnI NarI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1100 GGTACC G^GTACC Asp718I 1100 GGTACC G^GTACC AspHI 157 GAGCTC G[AT]GC[AT]^C AvaII 3 GGACC G^G[AT]CC BalI 386 TGGCCA TGG^CCA BamHI 101 GGATCC G^GATCC BglII 539 AGATCT A^GATCT BsaAI 241 TACGTA [CT]AC^GT[GA] BsaBI 511 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 780 CTGCAC GTGCAG(16/14) BsiHKAI 157 GAGCTC G[AT]GC[AT]^C BsmBI 869 CGTCTC CGTCTC(1/5) Bsp1407I 302 TGTACA T^GTACA BspMI 1042 ACCTGC ACCTGC(4/8) BsrFI 49 GCCGGC [GA]^CCGG[CT] BsrGI 302 TGTACA T^GTACA BstAPI 175 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 353 CATCC GGATG(2/0) CfoI 1084 GCGC GCG^C Cfr10I 49 GCCGGC [GA]^CCGG[CT] DrdI 178 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 506 CCATGG C^C[GA][CT]GG EarI 992 CTCTTC CTCTTC(1/4) EcoNI 482 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 267 ATGCAT ATGCA^T FauI 874 CCCGC CCCGC(4/6) FokI 353 CATCC GGATG(9/13) HhaI 1084 GCGC GCG^C HinP1I 1084 GCGC G^CGC KpnI 1100 GGTACC GGTAC^C Ksp632I 992 CTCTTC CTCTTC(1/4) MaeIII 918 GTCAC ^GT[ACGT]AC MamI 511 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 386 TGGCCA TGG^CCA MscI 386 TGGCCA TGG^CCA NaeI 49 GCCGGC GCC^GGC NcoI 506 CCATGG C^CATGG NdeI 713 CATATG CA^TATG NgoMIV 49 GCCGGC G^CCGGC NsiI 267 ATGCAT ATGCA^T PvuII 888 CAGCTG CAG^CTG SacI 157 GAGCTC GAGCT^C SfcI 144 CTGTAG C^T[GA][CT]AG SmaI 257 CCCGGG CCC^GGG SmlI 1207 CTCAAG C^T[CT][GA]AG SnaBI 241 TACGTA TAC^GTA SphI 265 GCATGC GCATG^C SspBI 302 TGTACA T^GTACA TaqI 188 TCGA T^CGA Tsp45I 918 GTCAC ^GT[GC]AC TthHB8I 188 TCGA T^CGA XmaCI 257 CCCGGG C^CCGGG XmaI 257 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 102 GATCC GGATC(4/5) 101 GGATC AvaI 257 CCCGGG C^[CT]CG[GA]G 533 CTCGGG BbsI 427 GTCTTC GAAGAC(2/6) 932 GTCTTC BbvCI 1159 CCTCAGC CCTCAGC(-5/-2) 1165 CCTCAGC BglI 642 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 893 GCCTGGAAGGC BlpI 552 GCTGAGC GC^T[ACGT]AGC 577 GCTGAGC Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC 577 GCTGAGC BpuAI 427 GTCTTC GAAGAC(2/6) 932 GTCTTC BsoBI 257 CCCGGG C^[CT]CG[GA]G 533 CTCGGG BsrI 131 ACTGG ACTGG(1/-1) 389 CCAGT BstYI 101 GGATCC [GA]^GATC[CT] 539 AGATCT CelII 552 GCTGAGC GC^T[ACGT]AGC 577 GCTGAGC MaeII 67 ACGT A^CGT 242 ACGT MflI 101 GGATCC [GA]^GATC[CT] 539 AGATCT MslI 378 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1105 CATACCTATG MspA1I 676 CAGCGG C[AC]G^C[GT]G 888 CAGCTG NspI 265 GCATGC [GA]CATG^[CT] 345 GCATGT XhoII 101 GGATCC [GA]^GATC[CT] 539 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AlwNI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BssKI BstNI BstXI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI Van91I