(Circular) (Six-base) MAPSORT of: pFN21AE2613.seq Check: 4850 from: 1 to: 5935 pFN21AE2613 5935 bp With 182 enzymes: SgfI * March 25, 2016 16:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3278 1937 Size: 1341 4594 Acc65I G'GTAC_C Cuts at: 3105 3256 3105 Size: 151 5784 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3998 5382 5650 1632 Size: 2366 1384 268 1917 Fragments arranged by size: 2366 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2933 1184 Size: 1749 4186 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2057 Size: 32 5903 AlwNI CAG_nnn'CTG Cuts at: 2095 2686 3102 3678 5250 2095 Size: 591 416 576 1572 2780 Fragments arranged by size: 2780 1572 591 576 416 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 3244 3470 3622 5731 1493 Size: 1751 226 152 2109 1697 Fragments arranged by size: 2109 1751 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2262 2538 3260 1870 Size: 73 319 276 722 4545 Fragments arranged by size: 4545 722 319 276 73 BamHI G'GATC_C Cuts at: 2106 3265 3632 2106 Size: 1159 367 4409 Fragments arranged by size: 4409 1159 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2325 3105 3148 3256 Size: 325 217 627 252 286 780 43 108 Cuts at: 3256 4711 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 780 627 325 286 252 217 108 43 BanII G_rGCy'C Cuts at: 1391 2022 2166 2672 2844 3146 3254 1391 Size: 631 144 506 172 302 108 4072 Fragments arranged by size: 4072 631 506 302 172 144 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2425 2930 928 Size: 971 526 505 3933 Fragments arranged by size: 3933 971 526 505 BbvCI CC'TCA_GC Cuts at: 3165 3171 3165 Size: 6 5929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4126 4160 1903 Size: 34 2189 34 3678 Fragments arranged by size: 3678 2189 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BfrBI ATG'CAT Cuts at: 2274 2274 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2653 2904 4546 Size: 107 122 71 631 605 981 251 1642 Cuts at: 4546 136 Size: 1525 Fragments arranged by size: 1642 1525 981 631 605 251 122 107 71 BglII A'GATC_T Cuts at: 2544 5930 2544 Size: 3386 2549 BlpI GC'TnA_GC Cuts at: 2558 2583 3352 2558 Size: 25 769 5141 Fragments arranged by size: 5141 769 25 Bme1580I G_kGCm'C Cuts at: 1790 2104 2475 3153 3996 5152 1790 Size: 314 371 678 843 1156 2573 Fragments arranged by size: 2573 1156 843 678 371 314 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 3666 4624 Size: 416 53 83 399 24 729 1520 958 Cuts at: 4624 442 Size: 1753 Fragments arranged by size: 1753 1520 958 729 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2152 2177 2186 2219 2660 2858 2875 3014 Size: 913 25 9 33 441 198 17 139 Cuts at: 3014 4595 1239 Size: 1581 2579 Fragments arranged by size: 2579 1581 913 441 198 139 33 25 17 9 Bpu10I CC'TnA_GC Cuts at: 3165 3171 3165 Size: 6 5929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3197 4060 4925 5223 3197 Size: 863 865 298 3909 Fragments arranged by size: 3909 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4598 882 Size: 617 3099 2219 Fragments arranged by size: 3099 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2248 3726 493 Size: 711 1044 1478 2702 Fragments arranged by size: 2702 1478 1044 711 BsaBI GATnn'nnATC Cuts at: 2520 5929 2520 Size: 3409 2526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4368 4766 5040 5187 5598 4368 Size: 398 274 147 411 4705 Fragments arranged by size: 4705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5710 Fragments arranged by size: 5710 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2435 2438 2441 2946 2979 1186 Size: 988 261 3 3 505 33 4142 Fragments arranged by size: 4142 988 505 261 33 3 3 BseYI C'CCAG_C Cuts at: 2130 2216 2456 5138 5634 2130 Size: 86 240 2682 496 2431 Fragments arranged by size: 2682 2431 496 240 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2770 1163 Size: 573 1034 4328 Fragments arranged by size: 4328 1034 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 3996 4081 5152 2166 Size: 1830 85 1071 2949 Fragments arranged by size: 2949 1830 1071 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 BsmBI CGTCTCn'nnnn_ Cuts at: 2880 2880 Size: 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2104 2166 2475 2672 2844 3146 Size: 399 232 82 62 309 197 172 302 Cuts at: 3146 3153 3254 3996 4081 5152 1391 Size: 7 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 309 302 232 197 172 101 85 82 62 7 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3056 3290 844 Size: 2212 234 3489 Fragments arranged by size: 3489 2212 234 BsrBI CCG'CTC Cuts at: 1394 1823 3822 1394 Size: 429 1999 3507 Fragments arranged by size: 3507 1999 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 2963 3182 4430 4604 65 Size: 1183 936 779 219 1248 174 1396 Fragments arranged by size: 1396 1248 1183 936 779 219 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 4579 4766 1700 Size: 110 244 2525 187 2869 Fragments arranged by size: 2869 2525 244 187 110 BsrGI T'GTAC_A Cuts at: 96 2307 96 Size: 2211 3724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 3995 5007 3995 Size: 1012 4923 BstAPI GCAn_nnn'nTGC Cuts at: 794 2186 794 Size: 1392 4543 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2389 2899 3116 2176 Size: 213 510 217 4995 Fragments arranged by size: 4995 510 217 213 BstYI r'GATC_y Cuts at: 1972 2106 2544 3265 3632 4017 4034 5486 5572 Size: 134 438 721 367 385 17 1452 86 Cuts at: 5572 5584 5930 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 721 438 385 367 346 134 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2511 513 Size: 552 939 507 3937 Fragments arranged by size: 3937 939 552 507 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 2734 3084 3451 4245 4265 2734 Size: 350 367 794 20 4404 Fragments arranged by size: 4404 794 367 350 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 3239 3239 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2189 4942 809 Size: 507 873 2753 1802 Fragments arranged by size: 2753 1802 873 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 2466 2754 Size: 54 1425 50 275 18 561 75 288 Cuts at: 2754 3382 4271 5900 8 Size: 628 889 1629 43 Fragments arranged by size: 1629 1425 889 628 561 288 275 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3003 3865 1353 Size: 1650 862 3423 Fragments arranged by size: 3423 1650 862 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2164 2164 Size: 5935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2177 2186 2219 2660 2858 2875 Size: 393 520 25 9 33 441 198 17 Cuts at: 2875 3014 3998 4595 5382 5650 1239 Size: 139 984 597 787 268 1524 Fragments arranged by size: 1524 984 787 597 520 441 393 268 198 139 33 25 17 9 EcoNI CCTnn'n_nnAGG Cuts at: 2491 2491 Size: 5935 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 4576 4608 4650 Size: 32 135 32 712 32 2260 32 42 Cuts at: 4650 4682 1373 Size: 32 2626 Fragments arranged by size: 2626 2260 712 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3279 3525 669 Size: 988 281 1341 246 3079 Fragments arranged by size: 3079 1341 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 3109 3260 3109 Size: 151 5784 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2547 5049 5233 1305 Size: 1242 2502 184 2007 Fragments arranged by size: 2502 2007 1242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 10 Size: 54 1425 343 561 3552 Fragments arranged by size: 3552 1425 561 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 3114 3893 4252 4411 518 Size: 648 1221 727 779 359 159 2042 Fragments arranged by size: 2042 1221 779 727 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2683 2895 3351 4028 5176 5421 1606 Size: 1077 212 456 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 677 456 245 212 NaeI GCC'GGC Cuts at: 2056 2056 Size: 5935 NcoI C'CATG_G Cuts at: 513 1065 2004 2511 513 Size: 552 939 507 3937 Fragments arranged by size: 3937 939 552 507 NdeI CA'TA_TG Cuts at: 387 2719 387 Size: 2332 3603 NgoMIV G'CCGG_C Cuts at: 2054 2054 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NsiI A_TGCA'T Cuts at: 2276 2276 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 2274 2354 3293 4838 1132 Size: 97 1045 80 939 1545 2229 Fragments arranged by size: 2229 1545 1045 939 97 80 PasI CC'CwG_GG Cuts at: 1348 2665 1348 Size: 1317 4618 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 2517 2686 3102 1108 Size: 1409 169 416 3941 Fragments arranged by size: 3941 1409 416 169 PfoI T'CCnGG_A Cuts at: 2061 2835 2061 Size: 774 5161 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PstI C_TGCA'G Cuts at: 830 3287 830 Size: 2457 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 PvuII CAG'CTG Cuts at: 2895 2895 Size: 5935 SacI G_AGCT'C Cuts at: 2166 2166 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SfcI C'TryA_G Cuts at: 826 1045 2149 3283 4418 5099 5290 826 Size: 219 1104 1134 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 1104 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 2264 3262 1872 Size: 392 998 4545 Fragments arranged by size: 4545 998 392 SmlI C'TyrA_G Cuts at: 820 1017 1943 3212 4039 4940 5202 820 Size: 197 926 1269 827 901 262 1553 Fragments arranged by size: 1553 1269 926 901 827 262 197 SnaBI TAC'GTA Cuts at: 493 2248 3726 493 Size: 1755 1478 2702 Fragments arranged by size: 2702 1755 1478 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 2274 3293 1132 Size: 1142 1019 3774 Fragments arranged by size: 3774 1142 1019 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 2511 2812 3137 3374 513 Size: 552 939 483 24 301 325 237 3074 Fragments arranged by size: 3074 939 552 483 325 301 237 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3962 3979 4132 4317 4762 325 Size: 1255 2382 17 153 185 445 1498 Fragments arranged by size: 2382 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 4181 5893 Size: 274 80 33 51 1443 330 1874 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1874 1712 1443 330 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5895 5927 5895 Size: 32 5903 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 2262 3260 1870 Size: 392 998 4545 Fragments arranged by size: 4545 998 392 XmnI GAAnn'nnTTC Cuts at: 4064 4064 Size: 5935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI