(Circular) (Six-base) MAPSORT of: pFN21AE3151.seq Check: 6900 from: 1 to: 5806 pFN21AE3151 5806 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2414 2485 2804 2414 Size: 71 319 5416 Fragments arranged by size: 5416 319 71 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3869 5253 5521 1632 Size: 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 268 AfeI AGC'GCT Cuts at: 2879 2891 2879 Size: 12 5794 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AlwNI CAG_nnn'CTG Cuts at: 2929 3549 5121 2929 Size: 620 1572 3614 Fragments arranged by size: 3614 1572 620 ApaI G_GGCC'C Cuts at: 2279 3017 2279 Size: 738 5068 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 3115 3341 3493 5602 1493 Size: 1622 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 226 152 AscI GG'CGCG_CC Cuts at: 3030 3030 Size: 5806 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2452 2620 3131 1870 Size: 73 126 383 168 511 4545 Fragments arranged by size: 4545 511 383 168 126 73 BamHI G'GATC_C Cuts at: 2332 3136 3503 2332 Size: 804 367 4635 Fragments arranged by size: 4635 804 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2125 2351 2371 2500 Size: 325 217 627 299 39 226 20 129 Cuts at: 2500 2509 3127 4582 618 Size: 9 618 1455 1842 Fragments arranged by size: 1842 1455 627 618 325 299 226 217 129 39 20 9 BanII G_rGCy'C Cuts at: 1391 2279 2453 2621 2868 3017 3125 1391 Size: 888 174 168 247 149 108 4072 Fragments arranged by size: 4072 888 247 174 168 149 108 BbeI G_GCGC'C Cuts at: 2090 2129 2504 2090 Size: 39 375 5392 Fragments arranged by size: 5392 375 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2062 3047 Size: 107 122 71 631 605 367 23 985 Cuts at: 3047 4417 136 Size: 1370 1525 Fragments arranged by size: 1525 1370 985 631 605 367 122 107 71 23 BglII A'GATC_T Cuts at: 2832 5801 2832 Size: 2969 2837 BlpI GC'TnA_GC Cuts at: 2439 2615 2915 3223 2439 Size: 176 300 308 5022 Fragments arranged by size: 5022 308 300 176 Bme1580I G_kGCm'C Cuts at: 1790 2012 2279 2382 2391 3017 3867 5023 1790 Size: 222 267 103 9 626 850 1156 2573 Fragments arranged by size: 2573 1156 850 626 267 222 103 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4466 1239 Size: 3227 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 2591 2831 2963 3931 4796 5094 2150 Size: 441 240 132 968 865 298 2862 Fragments arranged by size: 2862 968 865 441 298 240 132 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4469 882 Size: 617 2970 2219 Fragments arranged by size: 2970 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3597 493 Size: 711 2393 2702 Fragments arranged by size: 2702 2393 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2087 2126 2501 2546 Size: 53 83 186 936 554 39 375 45 Cuts at: 2546 2762 3613 3995 4630 275 Size: 216 851 382 635 1451 Fragments arranged by size: 1451 936 851 635 554 382 375 216 186 83 53 45 39 BsaWI w'CCGG_w Cuts at: 4239 4637 4911 5058 5469 4239 Size: 398 274 147 411 4576 Fragments arranged by size: 4576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2819 2849 1178 Size: 30 165 30 1416 30 4135 Fragments arranged by size: 4135 1416 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2087 3034 1186 Size: 840 61 947 3958 Fragments arranged by size: 3958 947 840 61 BseYI C'CCAG_C Cuts at: 2110 2485 5009 5505 2110 Size: 375 2524 496 2411 Fragments arranged by size: 2524 2411 496 375 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2561 1163 Size: 573 825 4408 Fragments arranged by size: 4408 825 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2174 2436 2769 3051 3072 3256 Size: 275 186 173 262 333 282 21 184 Cuts at: 3256 4017 5045 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 333 282 275 262 186 184 173 21 BsiHKAI G_wGCw'C Cuts at: 2184 3867 3952 5023 2184 Size: 1683 85 1071 2967 Fragments arranged by size: 2967 1683 1071 85 BsmI GAATG_Cn' Cuts at: 3317 3410 3317 Size: 93 5713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2184 2279 2382 2391 2453 2621 Size: 399 222 172 95 103 9 62 168 Cuts at: 2621 2868 3017 3125 3867 3952 5023 1391 Size: 247 149 108 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 247 222 172 168 149 108 103 95 85 62 9 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2414 2485 2733 2804 2953 3161 844 Size: 1570 71 248 71 149 208 3489 Fragments arranged by size: 3489 1570 248 208 149 71 71 BsrBI CCG'CTC Cuts at: 1394 1823 2438 2658 3024 3693 1394 Size: 429 615 220 366 669 3507 Fragments arranged by size: 3507 669 615 429 366 220 BsrDI GCAATG_nn' Cuts at: 65 1248 4301 4475 65 Size: 1183 3053 174 1396 Fragments arranged by size: 3053 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2263 2506 2552 4450 4637 1700 Size: 110 453 243 46 1898 187 2869 Fragments arranged by size: 2869 1898 453 243 187 110 46 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 3030 1925 Size: 1105 4701 BssSI C'ACGA_G Cuts at: 2858 3866 4878 2858 Size: 1008 1012 3786 Fragments arranged by size: 3786 1012 1008 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 794 Size: 2135 3671 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 5806 BstYI r'GATC_y Cuts at: 1972 2332 2832 3136 3503 3888 3905 5357 5443 Size: 360 500 304 367 385 17 1452 86 Cuts at: 5443 5455 5801 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 500 385 367 360 346 304 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2271 2391 2525 2644 513 Size: 552 939 90 177 120 134 119 3675 Fragments arranged by size: 3675 939 552 177 134 120 119 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 3322 4116 4136 3322 Size: 794 20 4992 Fragments arranged by size: 4992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2288 4813 809 Size: 507 972 2525 1802 Fragments arranged by size: 2525 1802 972 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2171 2214 Size: 54 1425 50 275 18 210 131 43 Cuts at: 2214 2433 2766 3048 3069 3253 4142 5771 8 Size: 219 333 282 21 184 889 1629 43 Fragments arranged by size: 1629 1425 889 333 282 275 219 210 184 131 54 50 43 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2171 2433 2766 3048 3069 3253 1537 Size: 275 359 262 333 282 21 184 4090 Fragments arranged by size: 4090 359 333 282 275 262 184 21 EarI CTCTTCn'nnn_ Cuts at: 1353 3736 1353 Size: 2383 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2212 2460 4393 4767 4913 387 Size: 1064 138 623 248 1933 374 146 1280 Fragments arranged by size: 1933 1280 1064 623 374 248 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3869 4466 5253 5521 1239 Size: 393 2237 597 787 268 1524 Fragments arranged by size: 2237 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2276 3013 2276 Size: 737 5069 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2896 2896 Size: 5806 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2090 2111 2129 2195 2209 2504 2881 2893 Size: 412 21 18 66 14 295 377 12 Cuts at: 2893 4953 1678 Size: 2060 2531 Fragments arranged by size: 2531 2060 412 377 295 66 21 18 14 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2747 2779 4447 Size: 32 135 32 830 32 313 32 1668 Cuts at: 4447 4479 4521 4553 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1668 830 313 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KasI G'GCGC_C Cuts at: 2086 2125 2500 2086 Size: 39 375 5392 Fragments arranged by size: 5392 375 39 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4920 5104 1305 Size: 3615 184 2007 Fragments arranged by size: 3615 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3764 4123 4282 518 Size: 648 2598 359 159 2042 Fragments arranged by size: 2598 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2393 2527 2646 2911 3006 3063 3222 Size: 490 297 134 119 265 95 57 159 Cuts at: 3222 3899 5047 5292 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 490 297 265 245 159 134 119 95 57 NaeI GCC'GGC Cuts at: 2265 2265 Size: 5806 NarI GG'CG_CC Cuts at: 2087 2126 2501 2087 Size: 39 375 5392 Fragments arranged by size: 5392 375 39 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4315 Fragments arranged by size: 4315 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NgoMIV G'CCGG_C Cuts at: 2263 2263 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 2433 3253 2433 Size: 820 4986 NspI r_CATG'y Cuts at: 1132 1229 3164 4709 1132 Size: 97 1935 1545 2229 Fragments arranged by size: 2229 1935 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 3084 1108 Size: 1976 3830 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PspOMI G'GGCC_C Cuts at: 2275 3013 2275 Size: 738 5068 PstI C_TGCA'G Cuts at: 830 2673 2781 3158 830 Size: 1843 108 377 3478 Fragments arranged by size: 3478 1843 377 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 3063 3063 Size: 5806 SacII CC_GC'GG Cuts at: 2097 2394 2528 2647 2097 Size: 297 134 119 5256 Fragments arranged by size: 5256 297 134 119 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SexAI A'CCwGG_T Cuts at: 2709 2709 Size: 5806 SfcI C'TryA_G Cuts at: 826 1045 2669 2777 3154 4289 4970 5161 826 Size: 219 1624 108 377 1135 681 191 1471 Fragments arranged by size: 1624 1471 1135 681 377 219 191 108 SfoI GGC'GCC Cuts at: 2088 2127 2502 2088 Size: 39 375 5392 Fragments arranged by size: 5392 375 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 2506 2552 1700 Size: 806 46 4954 Fragments arranged by size: 4954 806 46 SmaI CCC'GGG Cuts at: 1872 2622 3133 1872 Size: 750 511 4545 Fragments arranged by size: 4545 750 511 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 2606 2846 2978 3910 4811 Size: 197 926 222 441 240 132 932 901 Cuts at: 4811 5073 820 Size: 262 1553 Fragments arranged by size: 1553 932 926 901 441 262 240 222 197 132 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SrfI GCCC'GGGC Cuts at: 2622 2622 Size: 5806 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 2690 1517 Size: 1173 4633 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 3245 513 Size: 552 939 39 1202 3074 Fragments arranged by size: 3074 1202 939 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2272 3833 3850 4003 4188 4633 325 Size: 1255 692 1561 17 153 185 445 1498 Fragments arranged by size: 1561 1498 1255 692 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5766 5798 5766 Size: 32 5774 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2620 3131 1870 Size: 750 511 4545 Fragments arranged by size: 4545 750 511 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SwaI XcmI