Restriction map for
pFN21AE2180
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BbeI BbrPI BbsI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI MaeI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SalI SapI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1009 GTCTAC GT^[AC][GT]AC
AcyI 837 GACGCC G[GA]^CG[CT]C
Alw44I 424 GTGCAC G^TGCAC
AlwI 345 GATCC GGATC(4/5)
ApaI 936 GGGCCC GGGCC^C
ApaLI 424 GTGCAC G^TGCAC
AspI 1003 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 869 TGCGCA TGC^GCA
BbvCI 248 CCTCAGC CCTCAGC(-5/-2)
BcgI 263 GCATCCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 270 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 837 GACGCC G[GA]^CG[CT]C
BseRI 499 GAGGAG GAGGAG(10/8)
BsgI 520 CTGCAC GTGCAG(16/14)
BsmI 283 GAATGC GAATGC(1/-1)
BspMI 831 GCAGGT ACCTGC(4/8)
BsrDI 188 CATTGC GCAATG(2/0)
BstBI 1015 TTCGAA TT^CGAA
BstXI 58 CCAGCGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 344 AGATCC [GA]^GATC[CT]
EarI 1213 GAAGAG CTCTTC(1/4)
EcoNI 506 CCTTCCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 567 CCATGG C^C[AT][AT]GG
FspI 869 TGCGCA TGC^GCA
Hin1I 837 GACGCC G[GA]^CG[CT]C
Ksp632I 1213 GAAGAG CTCTTC(1/4)
MamI 270 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 344 AGATCC [GA]^GATC[CT]
MslI 232 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 567 CCATGG C^CATGG
NotI 1130 GCGGCCGC GC^GGCCGC
NspV 1015 TTCGAA TT^CGAA
PflFI 1003 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 572 GGGTCCC [GA]G^G[AT]CC[CT]
SbfI 612 CCTGCAGG CCTGCA^GG
SexAI 1004 ACCTGGT A^CC[AT]GGT
SfuI 1015 TTCGAA TT^CGAA
Sse8387I 612 CCTGCAGG CCTGCA^GG
StyI 567 CCATGG C^C[AT][AT]GG
TaqI 1016 TCGA T^CGA
Tsp509I 296 AATT ^AATT
Tth111I 1003 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1016 TCGA T^CGA
XcmI 122 CCAATGACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 344 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 410 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1239 CAGCGTCTG
BanI 948 GGCACC G^G[CT][GA]CC
1066 GGCACC
BanII 154 GAGCCC G[GA]GC[CT]^C
936 GGGCCC
BpmI 328 CTGGAG CTGGAG(16/14)
928 CTGGAG
BsiEI 901 CGGCCG CG[GA][CT]^CG
1131 CGGCCG
BsrI 534 ACTGG ACTGG(1/-1)
1190 ACTGG
BstAPI 1118 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1121 GCATGTGCTGC
DpnII 270 GATC ^GATC
345 GATC
DraII 557 AGGGCCT [GA]G^G[ACGT]CC[CT]
572 GGGTCCC
EagI 901 CGGCCG C^GGCCG
1131 CGGCCG
EclXI 901 CGGCCG C^GGCCG
1131 CGGCCG
Eco52I 901 CGGCCG C^GGCCG
1131 CGGCCG
EcoO109I 557 AGGGCCT [GA]G^G[ACGT]CC[CT]
572 GGGTCCC
KspI 52 CCGCGG CCGC^GG
1134 CCGCGG
MaeII 799 ACGT A^CGT
823 ACGT
MaeIII 230 GTCAC ^GT[ACGT]AC
835 GTGAC
MboI 270 GATC ^GATC
345 GATC
NdeII 270 GATC ^GATC
345 GATC
NspI 770 ACATGC [GA]CATG^[CT]
1121 GCATGT
SacII 52 CCGCGG CCGC^GG
1134 CCGCGG
Sau3AI 270 GATC ^GATC
345 GATC
SmaI 692 CCCGGG CCC^GGG
973 CCCGGG
SmlI 321 CTCAAG C^T[CT][GA]AG
817 CTCAAG
Tsp45I 230 GTCAC ^GT[GC]AC
835 GTGAC
XmaCI 692 CCCGGG C^CCGGG
973 CCCGGG
XmaI 692 CCCGGG C^CCGGG
973 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI Aor51HI AspHI
AvaI AvaII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraIII DsaI
EaeI Eco47III EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PleI PstI
PvuII Sau96I ScrFI SfaNI SfcI
StuI TseI TspRI