(Circular) (Six-base) MAPSORT of: pFN21AE2180.seq Check: 930 from: 1 to: 5956 pFN21AE2180 5956 bp With 182 enzymes: SgfI * March 28, 2012 13:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2827 2827 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3015 3299 1937 Size: 1078 284 4594 Fragments arranged by size: 4594 1078 284 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2356 2486 4019 5403 5671 1632 Size: 724 130 1533 1384 268 1917 Fragments arranged by size: 1917 1533 1384 724 268 130 AfeI AGC'GCT Cuts at: 2067 2315 2395 2672 3176 2067 Size: 248 80 277 504 4847 Fragments arranged by size: 4847 504 277 248 80 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 2420 3249 3699 5271 2420 Size: 829 450 1572 3105 Fragments arranged by size: 3105 1572 829 450 ApaI G_GGCC'C Cuts at: 2945 2945 Size: 5956 ApaLI G'TGCA_C Cuts at: 2429 4013 5169 2429 Size: 1584 1156 3216 Fragments arranged by size: 3216 1584 1156 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2697 2846 2978 3281 1870 Size: 73 754 149 132 303 4545 Fragments arranged by size: 4545 754 303 149 132 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2953 3071 3277 4732 618 Size: 325 217 627 1166 118 206 1455 1842 Fragments arranged by size: 1842 1455 1166 627 325 217 206 118 BanII G_rGCy'C Cuts at: 1391 2163 2945 3275 1391 Size: 772 782 330 4072 Fragments arranged by size: 4072 782 772 330 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BbvCI CC'TCA_GC Cuts at: 2254 2254 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2257 2291 4147 4181 1903 Size: 34 320 34 1856 34 3678 Fragments arranged by size: 3678 1856 320 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2433 2455 2945 2956 3074 4017 5173 1790 Size: 643 22 490 11 118 943 1156 2573 Fragments arranged by size: 2573 1156 943 643 490 118 22 11 BmgBI CAC'GTC Cuts at: 1318 2805 2829 1318 Size: 1487 24 4445 Fragments arranged by size: 4445 1487 24 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2954 4616 1239 Size: 1115 600 1662 2579 Fragments arranged by size: 2579 1662 1115 600 Bpu10I CC'TnA_GC Cuts at: 2204 2254 2567 2204 Size: 50 313 5593 Fragments arranged by size: 5593 313 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2311 2807 4081 4946 5244 2311 Size: 496 1274 865 298 3023 Fragments arranged by size: 3023 1274 865 496 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 2279 5950 2279 Size: 3671 2285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2843 3763 4145 4780 Size: 53 83 186 936 1310 920 382 635 Cuts at: 4780 275 Size: 1451 Fragments arranged by size: 1451 1310 936 920 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 1186 Size: 1333 4623 BseYI C'CCAG_C Cuts at: 2046 2052 2590 2944 5159 5655 2046 Size: 6 538 354 2215 496 2347 Fragments arranged by size: 2347 2215 538 496 354 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 1163 Size: 573 774 4609 Fragments arranged by size: 4609 774 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2909 3139 3406 4167 5195 1540 Size: 275 186 908 230 267 761 1028 2301 Fragments arranged by size: 2301 1028 908 761 275 267 230 186 BsiHKAI G_wGCw'C Cuts at: 2079 2169 2433 4017 4102 5173 2079 Size: 90 264 1584 85 1071 2862 Fragments arranged by size: 2862 1584 1071 264 90 85 BsmI GAATG_Cn' Cuts at: 2294 3467 3560 2294 Size: 1173 93 4690 Fragments arranged by size: 4690 1173 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2163 2169 2433 2455 2945 2956 Size: 399 289 84 6 264 22 490 11 Cuts at: 2956 3074 3275 4017 4102 5173 1391 Size: 118 201 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 289 264 201 118 85 84 22 11 6 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2827 3311 844 Size: 1983 484 3489 Fragments arranged by size: 3489 1983 484 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2192 4451 4625 65 Size: 1183 944 2259 174 1396 Fragments arranged by size: 2259 1396 1183 944 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 2962 3104 4600 4787 1700 Size: 110 398 754 142 1496 187 2869 Fragments arranged by size: 2869 1496 754 398 187 142 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 3129 3132 794 Size: 2335 3 3618 Fragments arranged by size: 3618 2335 3 BstBI TT'CG_AA Cuts at: 3021 4760 3021 Size: 1739 4217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2070 2070 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2349 3286 3653 4038 4055 5507 5593 5605 Size: 377 937 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 937 385 377 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2057 2572 2797 2859 3075 3139 Size: 552 939 53 515 225 62 216 64 Cuts at: 3139 513 Size: 3330 Fragments arranged by size: 3330 939 552 515 225 216 64 62 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2395 487 Size: 683 56 1169 4048 Fragments arranged by size: 4048 1169 683 56 BtsI GCAGTG_nn' Cuts at: 2546 2769 3181 3472 4266 4286 2546 Size: 223 412 291 794 20 4216 Fragments arranged by size: 4216 794 412 291 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DraIII CAC_nnn'GTG Cuts at: 2184 2437 3237 2184 Size: 253 800 4903 Fragments arranged by size: 4903 800 253 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2060 2906 2926 Size: 54 1425 50 275 18 230 846 20 Cuts at: 2926 3136 3142 3403 4292 5921 8 Size: 210 6 261 889 1629 43 Fragments arranged by size: 1629 1425 889 846 275 261 230 210 54 50 43 20 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2906 3136 3403 1537 Size: 275 1094 230 267 4090 Fragments arranged by size: 4090 1094 275 267 230 EarI CTCTTCn'nnn_ Cuts at: 1353 3213 3886 1353 Size: 1860 673 3423 Fragments arranged by size: 3423 1860 673 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2135 2797 4543 4917 5063 387 Size: 1064 138 546 662 1746 374 146 1280 Fragments arranged by size: 1746 1280 1064 662 546 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2356 2486 2954 4019 4616 5403 Size: 393 722 2 130 468 1065 597 787 Cuts at: 5403 5671 1239 Size: 268 1524 Fragments arranged by size: 1524 1065 787 722 597 468 393 268 130 2 EcoNI CCTnn'n_nnAGG Cuts at: 2515 2515 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2563 2578 2563 Size: 15 5941 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 2876 4462 2876 Size: 1586 4370 HaeII r_GCGC'y Cuts at: 1678 2016 2069 2317 2397 2674 2870 2976 3105 Size: 338 53 248 80 277 196 106 129 Cuts at: 3105 3178 5103 1678 Size: 73 1925 2531 Fragments arranged by size: 2531 1925 338 277 248 196 129 106 80 73 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4597 4629 4671 4703 1373 Size: 32 135 32 3025 32 42 32 2626 Fragments arranged by size: 3025 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2065 5070 5254 1305 Size: 760 3005 184 2007 Fragments arranged by size: 3005 2007 760 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2241 3914 4273 4432 518 Size: 648 1075 1673 359 159 2042 Fragments arranged by size: 2042 1673 1075 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2420 2814 2932 2948 3141 3372 4049 Size: 453 361 394 118 16 193 231 677 Cuts at: 4049 5197 5442 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 453 394 361 245 231 193 118 16 NaeI GCC'GGC Cuts at: 2210 2964 3106 2210 Size: 754 142 5060 Fragments arranged by size: 5060 754 142 NcoI C'CATG_G Cuts at: 513 1065 2004 2572 513 Size: 552 939 568 3897 Fragments arranged by size: 3897 939 568 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2208 2962 3104 2208 Size: 754 142 5060 Fragments arranged by size: 5060 754 142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3136 3403 3136 Size: 267 5689 NspI r_CATG'y Cuts at: 1132 1229 2779 3130 3314 4859 1132 Size: 97 1550 351 184 1545 2229 Fragments arranged by size: 2229 1550 1545 351 184 97 PasI CC'CwG_GG Cuts at: 1348 2455 1348 Size: 1107 4849 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PfoI T'CCnGG_A Cuts at: 2580 2580 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 2578 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2941 2941 Size: 5956 PstI C_TGCA'G Cuts at: 830 2553 2622 2648 3308 830 Size: 1723 69 26 660 3478 Fragments arranged by size: 3478 1723 660 69 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2420 2814 2932 2420 Size: 394 118 5444 Fragments arranged by size: 5444 394 118 SacII CC_GC'GG Cuts at: 2060 3142 2060 Size: 1082 4874 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SanDI GG'GwC_CC Cuts at: 2578 2578 Size: 5956 SbfI CC_TGCA'GG Cuts at: 2622 3308 2622 Size: 686 5270 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SexAI A'CCwGG_T Cuts at: 3009 3009 Size: 5956 SfcI C'TryA_G Cuts at: 826 1045 2549 2618 2644 3304 4439 5120 5311 Size: 219 1504 69 26 660 1135 681 191 Cuts at: 5311 826 Size: 1471 Fragments arranged by size: 1504 1471 1135 681 660 219 191 69 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 2699 2980 3283 1872 Size: 827 281 303 4545 Fragments arranged by size: 4545 827 303 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 2326 2822 4060 4961 5223 820 Size: 197 926 383 496 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 496 383 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 2202 2471 2510 2616 1517 Size: 685 269 39 106 4857 Fragments arranged by size: 4857 685 269 106 39 StyI C'CwwG_G Cuts at: 513 1065 2004 2572 3395 513 Size: 552 939 568 823 3074 Fragments arranged by size: 3074 939 823 568 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 2140 2604 3983 4000 4153 4338 Size: 1255 482 78 464 1379 17 153 185 Cuts at: 4338 4783 325 Size: 445 1498 Fragments arranged by size: 1498 1379 1255 482 464 445 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2423 2455 5916 5948 2423 Size: 32 3461 32 2431 Fragments arranged by size: 3461 2431 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3011 1316 Size: 1695 4261 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2134 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 2697 2978 3281 1870 Size: 827 281 303 4545 Fragments arranged by size: 4545 827 303 281 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI