(Linear) (Six-base) MAPSORT of: KIEE2180.seq Check: 9559 from: 1 to: 1251 KIEE2180.orf With 182 enzymes: SgfI * March 22, 2012 15:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 822 1251 Size: 822 429 AccI GT'mk_AC Cuts at: 0 1010 1251 Size: 1010 241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 481 1251 Size: 351 130 770 Fragments arranged by size: 770 351 130 AfeI AGC'GCT Cuts at: 0 62 310 390 667 1171 1251 Size: 62 248 80 277 504 80 Fragments arranged by size: 504 277 248 80 80 62 AlwNI CAG_nnn'CTG Cuts at: 0 415 1244 1251 Size: 415 829 7 Fragments arranged by size: 829 415 7 ApaI G_GGCC'C Cuts at: 0 940 1251 Size: 940 311 ApaLI G'TGCA_C Cuts at: 0 424 1251 Size: 424 827 AvaI C'yCGr_G Cuts at: 0 692 841 973 1251 Size: 692 149 132 278 Fragments arranged by size: 692 278 149 132 BanI G'GyrC_C Cuts at: 0 948 1066 1251 Size: 948 118 185 Fragments arranged by size: 948 185 118 BanII G_rGCy'C Cuts at: 0 158 940 1251 Size: 158 782 311 Fragments arranged by size: 782 311 158 BbvCI CC'TCA_GC Cuts at: 0 249 1251 Size: 249 1002 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 252 286 1251 Size: 252 34 965 Fragments arranged by size: 965 252 34 Bme1580I G_kGCm'C Cuts at: 0 428 450 940 951 1069 1251 Size: 428 22 490 11 118 182 Fragments arranged by size: 490 428 182 118 22 11 BmgBI CAC'GTC Cuts at: 0 800 824 1251 Size: 800 24 427 Fragments arranged by size: 800 427 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 949 1251 Size: 349 600 302 Fragments arranged by size: 600 349 302 Bpu10I CC'TnA_GC Cuts at: 0 199 249 562 1251 Size: 199 50 313 689 Fragments arranged by size: 689 313 199 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 802 1251 Size: 306 496 449 Fragments arranged by size: 496 449 306 BsaBI GATnn'nnATC Cuts at: 0 274 1251 Size: 274 977 BsaHI Gr'CG_yC Cuts at: 0 838 1251 Size: 838 413 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 514 1251 Size: 514 737 BseYI C'CCAG_C Cuts at: 0 41 47 585 939 1251 Size: 41 6 538 354 312 Fragments arranged by size: 538 354 312 41 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 1251 Size: 505 746 BsiEI CG_ry'CG Cuts at: 0 904 1134 1251 Size: 904 230 117 Fragments arranged by size: 904 230 117 BsiHKAI G_wGCw'C Cuts at: 0 74 164 428 1251 Size: 74 90 264 823 Fragments arranged by size: 823 264 90 74 BsmI GAATG_Cn' Cuts at: 0 289 1251 Size: 289 962 Bsp1286I G_dGCh'C Cuts at: 0 74 158 164 428 450 940 951 1069 Size: 74 84 6 264 22 490 11 118 Cuts at: 1069 1251 Size: 182 Fragments arranged by size: 490 264 182 118 84 74 22 11 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 822 1251 Size: 822 429 BsrDI GCAATG_nn' Cuts at: 0 187 1251 Size: 187 1064 BsrFI r'CCGG_y Cuts at: 0 203 957 1099 1251 Size: 203 754 142 152 Fragments arranged by size: 754 203 152 142 BstAPI GCAn_nnn'nTGC Cuts at: 0 1124 1127 1251 Size: 1124 3 124 Fragments arranged by size: 1124 124 3 BstBI TT'CG_AA Cuts at: 0 1016 1251 Size: 1016 235 BstXI CCAn_nnnn'nTGG Cuts at: 0 65 1251 Size: 65 1186 BstYI r'GATC_y Cuts at: 0 344 1251 Size: 344 907 BtgI C'CryG_G Cuts at: 0 52 567 792 854 1070 1134 1251 Size: 52 515 225 62 216 64 117 Fragments arranged by size: 515 225 216 117 64 62 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 390 1251 Size: 390 861 BtsI GCAGTG_nn' Cuts at: 0 541 764 1176 1251 Size: 541 223 412 75 Fragments arranged by size: 541 412 223 75 DraIII CAC_nnn'GTG Cuts at: 0 179 432 1232 1251 Size: 179 253 800 19 Fragments arranged by size: 800 253 179 19 EaeI y'GGCC_r Cuts at: 0 55 901 921 1131 1137 1251 Size: 55 846 20 210 6 114 Fragments arranged by size: 846 210 114 55 20 6 EagI C'GGCC_G Cuts at: 0 901 1131 1251 Size: 901 230 120 Fragments arranged by size: 901 230 120 EarI CTCTTCn'nnn_ Cuts at: 0 1208 1251 Size: 1208 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 130 792 1251 Size: 130 662 459 Fragments arranged by size: 662 459 130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 351 481 949 1251 Size: 349 2 130 468 302 Fragments arranged by size: 468 349 302 130 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 510 1251 Size: 510 741 EcoO109I rG'GnC_Cy Cuts at: 0 558 573 1251 Size: 558 15 678 Fragments arranged by size: 678 558 15 FspI TGC'GCA Cuts at: 0 871 1251 Size: 871 380 HaeII r_GCGC'y Cuts at: 0 11 64 312 392 669 865 971 1100 Size: 11 53 248 80 277 196 106 129 Cuts at: 1100 1173 1251 Size: 73 78 Fragments arranged by size: 277 248 196 129 106 80 78 73 53 11 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 60 1251 Size: 60 1191 MslI CAynn'nnrTG Cuts at: 0 236 1251 Size: 236 1015 MspA1I CmG'CkG Cuts at: 0 54 415 809 927 943 1136 1251 Size: 54 361 394 118 16 193 115 Fragments arranged by size: 394 361 193 118 115 54 16 NaeI GCC'GGC Cuts at: 0 205 959 1101 1251 Size: 205 754 142 150 Fragments arranged by size: 754 205 150 142 NcoI C'CATG_G Cuts at: 0 567 1251 Size: 567 684 NgoMIV G'CCGG_C Cuts at: 0 203 957 1099 1251 Size: 203 754 142 152 Fragments arranged by size: 754 203 152 142 NotI GC'GGCC_GC Cuts at: 0 1131 1251 Size: 1131 120 NspI r_CATG'y Cuts at: 0 774 1125 1251 Size: 774 351 126 Fragments arranged by size: 774 351 126 PasI CC'CwG_GG Cuts at: 0 450 1251 Size: 450 801 PfoI T'CCnGG_A Cuts at: 0 575 1251 Size: 575 676 PpuMI rG'GwC_Cy Cuts at: 0 573 1251 Size: 573 678 PspOMI G'GGCC_C Cuts at: 0 936 1251 Size: 936 315 PstI C_TGCA'G Cuts at: 0 548 617 643 1251 Size: 548 69 26 608 Fragments arranged by size: 608 548 69 26 PvuII CAG'CTG Cuts at: 0 415 809 927 1251 Size: 415 394 118 324 Fragments arranged by size: 415 394 324 118 SacII CC_GC'GG Cuts at: 0 55 1137 1251 Size: 55 1082 114 Fragments arranged by size: 1082 114 55 SanDI GG'GwC_CC Cuts at: 0 573 1251 Size: 573 678 SbfI CC_TGCA'GG Cuts at: 0 617 1251 Size: 617 634 SexAI A'CCwGG_T Cuts at: 0 1004 1251 Size: 1004 247 SfcI C'TryA_G Cuts at: 0 544 613 639 1251 Size: 544 69 26 612 Fragments arranged by size: 612 544 69 26 SmaI CCC'GGG Cuts at: 0 694 975 1251 Size: 694 281 276 Fragments arranged by size: 694 281 276 SmlI C'TyrA_G Cuts at: 0 321 817 1251 Size: 321 496 434 Fragments arranged by size: 496 434 321 StuI AGG'CCT Cuts at: 0 197 466 505 611 1251 Size: 197 269 39 106 640 Fragments arranged by size: 640 269 197 106 39 StyI C'CwwG_G Cuts at: 0 567 1251 Size: 567 684 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 57 135 599 1251 Size: 57 78 464 652 Fragments arranged by size: 652 464 78 57 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 418 450 1251 Size: 418 32 801 Fragments arranged by size: 801 418 32 Tth111I GACn'n_nGTC Cuts at: 0 1006 1251 Size: 1006 245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 129 1251 Size: 129 1122 XmaI C'CCGG_G Cuts at: 0 692 973 1251 Size: 692 281 278 Fragments arranged by size: 692 281 278 Enzymes that do cut: AarI AccI AcuI AfeI AlwNI ApaI ApaLI AvaI BanI BanII BbvCI BcgI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII MmeI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PfoI PpuMI PspOMI PstI PvuII SacII SanDI SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI BsaI BsaAI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI