################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AviII BcgI BciVI BclI BfrI BglI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KspI MamI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 403 GACGTC GACGT^C Acc65I 1230 GGTACC G^GTACC AccI 1492 GTCTAC GT^[AC][GT]AC AccIII 350 TCCGGA T^CCGGA ApaI 1534 GGGCCC GGGCC^C Asp700I 471 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1230 GGTACC G^GTACC AspHI 2335 GAGCTC G[AT]GC[AT]^C AvrII 1071 CCTAGG C^CTAGG BamHI 2142 GGATCC G^GATCC BbeI 1570 GGCGCC GGCGC^C BbsI 1972 GTCTTC GAAGAC(2/6) BbvCI 2227 GCTGAGG CCTCAGC(-5/-2) BglII 881 AGATCT A^GATCT BlnI 1071 CCTAGG C^CTAGG BlpI 9 GCTGAGC GC^T[ACGT]AGC Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC BpuAI 1972 GTCTTC GAAGAC(2/6) BseAI 350 TCCGGA T^CCGGA BsiHKAI 2335 GAGCTC G[AT]GC[AT]^C BspEI 350 TCCGGA T^CCGGA BsrFI 738 GCCGGC [GA]^CCGG[CT] CelII 9 GCTGAGC GC^T[ACGT]AGC Cfr10I 738 GCCGGC [GA]^CCGG[CT] DraIII 1084 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1097 CCGTGG C^C[GA][CT]GG EcoNI 861 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1138 ATGCAT ATGCA^T KasI 1570 GGCGCC G^GCGCC KpnI 1230 GGTACC GGTAC^C MroI 350 TCCGGA T^CCGGA NaeI 738 GCCGGC GCC^GGC NarI 1570 GGCGCC GG^CGCC NgoMIV 738 GCCGGC G^CCGGC NsiI 1138 ATGCAT ATGCA^T PstI 55 CTGCAG CTGCA^G SacI 2335 GAGCTC GAGCT^C SapI 1032 GCTCTTC GCTCTTC(1/4) SfoI 1570 GGCGCC GGC^GCC SmaI 48 CCCGGG CCC^GGG XmaCI 48 CCCGGG C^CCGGG XmaI 48 CCCGGG C^CCGGG XmnI 471 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 289 CGCG CG^CG 1604 CGCG AcyI 403 GACGTC G[GA]^CG[CT]C 1570 GGCGCC AflIII 887 ACGTGT A^C[GA][CT]GT 2252 ACGTGT AlwNI 409 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 829 CAGGACCTG AocI 1064 CCTCAGG CC^T[ACGT]AGG 1150 CCTCAGG AvaI 48 CCCGGG C^[CT]CG[GA]G 2341 CCCGAG BbrPI 382 CACGTG CAC^GTG 682 CACGTG BsaHI 403 GACGTC G[GA]^CG[CT]C 1570 GGCGCC BsiEI 713 CGGCCG CG[GA][CT]^CG 1016 CGGCCG BsoBI 48 CCCGGG C^[CT]CG[GA]G 2341 CCCGAG BsrBI 601 GAGCGG CCGCTC(-3/-3) 1696 GAGCGG BssSI 844 CACGAG CACGAG(-5/-1) 2104 CACGAG BstUI 289 CGCG CG^CG 1604 CGCG Bsu36I 1064 CCTCAGG CC^T[ACGT]AGG 1150 CCTCAGG EagI 713 CGGCCG C^GGCCG 1016 CGGCCG EclXI 713 CGGCCG C^GGCCG 1016 CGGCCG Eco52I 713 CGGCCG C^GGCCG 1016 CGGCCG Eco81I 1064 CCTCAGG CC^T[ACGT]AGG 1150 CCTCAGG HgaI 1481 GACGC GACGC(5/10) 1051 GCGTC Hin1I 403 GACGTC G[GA]^CG[CT]C 1570 GGCGCC HindIII 114 AAGCTT A^AGCTT 514 AAGCTT MvnI 289 CGCG CG^CG 1604 CGCG PmaCI 382 CACGTG CAC^GTG 682 CACGTG PmlI 382 CACGTG CAC^GTG 682 CACGTG PvuII 230 CAGCTG CAG^CTG 2417 CAGCTG SexAI 1841 ACCTGGT A^CC[AT]GGT 1967 ACCAGGT TfiI 789 GAATC G^A[AT]TC 1240 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AfaI AluI AlwI AspI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII