(Circular) (Six-base) MAPSORT of: pFN21AE2651.seq Check: 9795 from: 1 to: 7186 pFN21AE2651 7186 bp With 182 enzymes: SgfI * November 16, 2016 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3196 3196 Size: 7186 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2412 4996 278 Size: 53 83 186 936 876 2584 2468 Fragments arranged by size: 2584 2468 936 876 186 83 53 AccI GT'mk_AC Cuts at: 1937 3498 4529 1937 Size: 1561 1031 4594 Fragments arranged by size: 4594 1561 1031 Acc65I G'GTAC_C Cuts at: 3235 4507 3235 Size: 1272 5914 AclI AA'CG_TT Cuts at: 1563 5313 5686 1563 Size: 3750 373 3063 Fragments arranged by size: 3750 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 2732 2903 4208 5249 6633 6901 1632 Size: 659 441 171 1305 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1305 1041 659 441 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6989 AflIII A'CryG_T Cuts at: 1203 2892 4257 6085 7062 1203 Size: 1689 1365 1828 977 1327 Fragments arranged by size: 1828 1689 1365 1327 977 AgeI A'CCGG_T Cuts at: 6017 6017 Size: 7186 AhdI GACnn_n'nnGTC Cuts at: 5915 5915 Size: 7186 AleI CACnn'nnGTG Cuts at: 1166 2735 2990 1166 Size: 1569 255 5362 Fragments arranged by size: 5362 1569 255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7186 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3935 3967 3935 Size: 32 7154 AlwNI CAG_nnn'CTG Cuts at: 2419 2839 4929 6501 2419 Size: 420 2090 1572 3104 Fragments arranged by size: 3104 2090 1572 420 ApaI G_GGCC'C Cuts at: 3543 3543 Size: 7186 ApaLI G'TGCA_C Cuts at: 5243 6399 5243 Size: 1156 6030 ApoI r'AATT_y Cuts at: 1493 4495 4721 4873 6982 1493 Size: 3002 226 152 2109 1697 Fragments arranged by size: 3002 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5740 160 Size: 872 4708 1606 Fragments arranged by size: 4708 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7186 AvaI C'yCGr_G Cuts at: 1870 1943 2053 4346 4511 1870 Size: 73 110 2293 165 4545 Fragments arranged by size: 4545 2293 165 110 73 AvrII C'CTAG_G Cuts at: 3076 3076 Size: 7186 BamHI G'GATC_C Cuts at: 4147 4516 4883 4147 Size: 369 367 6450 Fragments arranged by size: 6450 369 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 3235 3509 3575 4507 Size: 325 217 627 305 1143 274 66 932 Cuts at: 4507 5962 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1143 932 627 325 305 274 217 66 BanII G_rGCy'C Cuts at: 1391 3101 3543 4116 4344 4505 1391 Size: 1710 442 573 228 161 4072 Fragments arranged by size: 4072 1710 573 442 228 161 BbeI G_GCGC'C Cuts at: 3579 3579 Size: 7186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3970 928 Size: 971 2071 4144 Fragments arranged by size: 4144 2071 971 BbvCI CC'TCA_GC Cuts at: 4233 4233 Size: 7186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5377 5411 1903 Size: 34 3440 34 3678 Fragments arranged by size: 3678 3440 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5078 6288 5078 Size: 1210 5976 BclI T'GATC_A Cuts at: 1548 1548 Size: 7186 BfrBI ATG'CAT Cuts at: 3145 3145 Size: 7186 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5797 136 Size: 107 122 71 631 605 4125 1525 Fragments arranged by size: 4125 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2886 7181 2886 Size: 4295 2891 BlpI GC'TnA_GC Cuts at: 2015 4603 2015 Size: 2588 4598 Bme1580I G_kGCm'C Cuts at: 1790 2727 3543 5247 6403 1790 Size: 937 816 1704 1156 2573 Fragments arranged by size: 2573 1704 1156 937 816 BmgBI CAC'GTC Cuts at: 1318 4258 1318 Size: 2940 4246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3649 3897 4917 Size: 416 53 83 399 24 2232 248 1020 Cuts at: 4917 5875 442 Size: 958 1753 Fragments arranged by size: 2232 1753 1020 958 416 399 248 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7186 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 7186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 4237 4331 4404 5846 1239 Size: 1236 1762 94 73 1442 2579 Fragments arranged by size: 2579 1762 1442 1236 94 73 Bpu10I CC'TnA_GC Cuts at: 4233 4233 Size: 7186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2735 3632 4034 4448 5311 6176 6474 2735 Size: 897 402 414 863 865 298 3447 Fragments arranged by size: 3447 897 865 863 414 402 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2387 2592 3527 4073 5849 882 Size: 617 888 205 935 546 1776 2219 Fragments arranged by size: 2219 1776 935 888 617 546 205 BsaAI yAC'GTr Cuts at: 493 1204 2389 2689 2893 4977 493 Size: 711 1185 300 204 2084 2702 Fragments arranged by size: 2702 2084 1185 711 300 204 BsaBI GATnn'nnATC Cuts at: 7180 7180 Size: 7186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2409 3576 4993 5375 Size: 53 83 186 936 876 1167 1417 382 Cuts at: 5375 6010 275 Size: 635 1451 Fragments arranged by size: 1451 1417 1167 936 876 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2355 2542 3063 5619 6017 6291 6438 6849 2355 Size: 187 521 2556 398 274 147 411 2692 Fragments arranged by size: 2692 2556 521 411 398 274 187 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3719 3749 1178 Size: 30 165 30 2316 30 4615 Fragments arranged by size: 4615 2316 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4022 4025 4352 1186 Size: 2836 3 327 4020 Fragments arranged by size: 4020 2836 327 3 BseYI C'CCAG_C Cuts at: 2692 3168 3604 4061 4361 6389 6885 2692 Size: 476 436 457 300 2028 496 2993 Fragments arranged by size: 2993 2028 496 476 457 436 300 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2742 3020 3224 4020 1163 Size: 573 570 436 278 204 796 4329 Fragments arranged by size: 4329 796 573 570 436 278 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2721 3024 4636 5397 6425 1540 Size: 275 186 720 303 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 720 303 275 186 BsiHKAI G_wGCw'C Cuts at: 4344 5247 5332 6403 4344 Size: 903 85 1071 5127 Fragments arranged by size: 5127 1071 903 85 BsmI GAATG_Cn' Cuts at: 4697 4790 4697 Size: 93 7093 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2727 3101 3543 4116 4344 4505 5247 Size: 399 937 374 442 573 228 161 742 Cuts at: 5247 5332 6403 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 573 442 399 374 228 161 85 BspEI T'CCGG_A Cuts at: 2355 6849 2355 Size: 4494 2692 BspHI T'CATG_A Cuts at: 5075 6805 5075 Size: 1730 5456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2847 3196 3294 3336 4541 844 Size: 2003 349 98 42 1205 3489 Fragments arranged by size: 3489 2003 1205 349 98 42 BsrBI CCG'CTC Cuts at: 1394 1823 2608 3703 5073 1394 Size: 429 785 1095 1370 3507 Fragments arranged by size: 3507 1370 1095 785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5681 5855 65 Size: 1183 4433 174 1396 Fragments arranged by size: 4433 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2743 5830 6017 1700 Size: 110 933 3087 187 2869 Fragments arranged by size: 3087 2869 933 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7186 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7186 BssSI C'ACGA_G Cuts at: 2849 4109 5246 6258 2849 Size: 1260 1137 1012 3777 Fragments arranged by size: 3777 1260 1137 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 3328 4034 794 Size: 1298 1236 706 3946 Fragments arranged by size: 3946 1298 1236 706 BstBI TT'CG_AA Cuts at: 5990 5990 Size: 7186 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7186 BstXI CCAn_nnnn'nTGG Cuts at: 2089 2257 2537 3269 3572 4093 2089 Size: 168 280 732 303 521 5182 Fragments arranged by size: 5182 732 521 303 280 168 BstYI r'GATC_y Cuts at: 1972 2592 2886 3432 3909 4147 4516 4883 5268 Size: 620 294 546 477 238 369 367 385 Cuts at: 5268 5285 6737 6823 6835 7181 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 620 546 477 385 369 367 346 294 238 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3070 3156 3070 Size: 86 7100 BtgI C'CryG_G Cuts at: 513 1065 3102 513 Size: 552 2037 4597 Fragments arranged by size: 4597 2037 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6447 Fragments arranged by size: 6447 683 56 BtsI GCAGTG_nn' Cuts at: 4032 4227 4702 5496 5516 4032 Size: 195 475 794 20 5702 Fragments arranged by size: 5702 794 475 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7186 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7151 DraI TTT'AAA Cuts at: 4490 4490 Size: 7186 DraIII CAC_nnn'GTG Cuts at: 3094 3094 Size: 7186 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6193 809 Size: 507 4877 1802 Fragments arranged by size: 4877 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 2583 2718 Size: 54 1425 50 275 18 373 380 135 Cuts at: 2718 2822 3021 3105 3711 3900 4633 5522 7151 Size: 104 199 84 606 189 733 889 1629 Cuts at: 7151 8 Size: 43 Fragments arranged by size: 1629 1425 889 733 606 380 373 275 199 189 135 104 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2718 3021 4633 1537 Size: 275 906 303 1612 4090 Fragments arranged by size: 4090 1612 906 303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2471 3044 3933 5116 1353 Size: 1118 573 889 1183 3423 Fragments arranged by size: 3423 1183 1118 889 573 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4208 5773 6147 6293 387 Size: 1064 138 2619 1565 374 146 1280 Fragments arranged by size: 2619 1565 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4342 4342 Size: 7186 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2475 2732 2903 4208 4237 4331 Size: 393 659 184 257 171 1305 29 94 Cuts at: 4331 4404 5249 5846 6633 6901 1239 Size: 73 845 597 787 268 1524 Fragments arranged by size: 1524 1305 845 787 659 597 393 268 257 184 171 94 73 29 EcoNI CCTnn'n_nnAGG Cuts at: 2870 2870 Size: 7186 EcoO109I rG'GnC_Cy Cuts at: 2836 2878 3439 3540 3601 3808 4183 2836 Size: 42 561 101 61 207 375 5839 Fragments arranged by size: 5839 561 375 207 101 61 42 EcoRI G'AATT_C Cuts at: 4495 4873 4495 Size: 378 6808 FspI TGC'GCA Cuts at: 5692 5692 Size: 7186 HaeII r_GCGC'y Cuts at: 1678 2022 3579 3895 6333 1678 Size: 344 1557 316 2438 2531 Fragments arranged by size: 2531 2438 1557 344 316 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3122 3154 3719 3751 5827 Size: 32 135 32 1550 32 565 32 2076 Cuts at: 5827 5859 5901 5933 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2076 1550 565 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4530 4776 669 Size: 988 281 2592 246 3079 Fragments arranged by size: 3079 2592 988 281 246 HindIII A'AGCT_T Cuts at: 748 2119 2519 748 Size: 1371 400 5415 Fragments arranged by size: 5415 1371 400 HpaI GTT'AAC Cuts at: 4776 4776 Size: 7186 KasI G'GCGC_C Cuts at: 3575 3575 Size: 7186 KpnI G_GTAC'C Cuts at: 3239 4511 3239 Size: 1272 5914 MfeI C'AATT_G Cuts at: 4785 4785 Size: 7186 MluI A'CGCG_T Cuts at: 7062 7062 Size: 7186 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3084 3903 6300 6484 1305 Size: 1779 819 2397 184 2007 Fragments arranged by size: 2397 2007 1779 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2205 2585 3107 3713 3902 Size: 54 1425 343 373 380 522 606 189 Cuts at: 3902 10 Size: 3294 Fragments arranged by size: 3294 1425 606 522 380 373 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2255 2555 2735 2990 3140 3267 Size: 648 921 168 300 180 255 150 127 Cuts at: 3267 4091 4094 5144 5503 5662 518 Size: 824 3 1050 359 159 2042 Fragments arranged by size: 2042 1050 921 824 648 359 300 255 180 168 159 150 127 3 MspA1I CmG'CkG Cuts at: 1606 2237 4065 4424 4602 5279 6427 6672 1606 Size: 631 1828 359 178 677 1148 245 2120 Fragments arranged by size: 2120 1828 1148 677 631 359 245 178 NaeI GCC'GGC Cuts at: 2745 2745 Size: 7186 NarI GG'CG_CC Cuts at: 3576 3576 Size: 7186 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6634 NdeI CA'TA_TG Cuts at: 387 387 Size: 7186 NgoMIV G'CCGG_C Cuts at: 2743 2743 Size: 7186 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7186 NotI GC'GGCC_GC Cuts at: 4633 4633 Size: 7186 NsiI A_TGCA'T Cuts at: 3147 3147 Size: 7186 NspI r_CATG'y Cuts at: 1132 1229 2429 3149 4001 4544 6089 1132 Size: 97 1200 720 852 543 1545 2229 Fragments arranged by size: 2229 1545 1200 852 720 543 97 PasI CC'CwG_GG Cuts at: 1348 3796 1348 Size: 2448 4738 PciI A'CATG_T Cuts at: 6085 6085 Size: 7186 PflMI CCAn_nnn'nTGG Cuts at: 1108 2692 3094 3786 1108 Size: 1584 402 692 4508 Fragments arranged by size: 4508 1584 692 402 PfoI T'CCnGG_A Cuts at: 2471 2505 2865 3399 3737 2471 Size: 34 360 534 338 5920 Fragments arranged by size: 5920 534 360 338 34 PmeI GTTT'AAAC Cuts at: 4490 4490 Size: 7186 PmlI CAC'GTG Cuts at: 2389 2689 2389 Size: 300 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5255 5287 6768 6800 5255 Size: 32 1481 32 5641 Fragments arranged by size: 5641 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2836 3439 3601 4183 2836 Size: 603 162 582 5839 Fragments arranged by size: 5839 603 582 162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7186 PsiI TTA'TAA Cuts at: 4756 4756 Size: 7186 PspOMI G'GGCC_C Cuts at: 3539 3539 Size: 7186 PstI C_TGCA'G Cuts at: 830 2064 4538 830 Size: 1234 2474 3478 Fragments arranged by size: 3478 2474 1234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7186 PvuII CAG'CTG Cuts at: 2237 4424 2237 Size: 2187 4999 SacI G_AGCT'C Cuts at: 4344 4344 Size: 7186 SalI G'TCGA_C Cuts at: 1936 4528 1936 Size: 2592 4594 SanDI GG'GwC_CC Cuts at: 3439 3439 Size: 7186 SapI GCTCTTCn'nnn_ Cuts at: 3044 3044 Size: 7186 SbfI CC_TGCA'GG Cuts at: 4538 4538 Size: 7186 ScaI AGT'ACT Cuts at: 5434 7146 5434 Size: 1712 5474 SexAI A'CCwGG_T Cuts at: 3846 3972 3846 Size: 126 7060 SfcI C'TryA_G Cuts at: 826 1045 2060 3833 4332 4534 5669 6350 6541 Size: 219 1015 1773 499 202 1135 681 191 Cuts at: 6541 826 Size: 1471 Fragments arranged by size: 1773 1471 1135 1015 681 499 219 202 191 SfoI GGC'GCC Cuts at: 3577 3577 Size: 7186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7186 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7186 SmaI CCC'GGG Cuts at: 1872 2055 4513 1872 Size: 183 2458 4545 Fragments arranged by size: 4545 2458 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2750 3647 4049 4463 5290 6191 Size: 197 926 807 897 402 414 827 901 Cuts at: 6191 6453 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 897 827 807 414 402 262 197 SnaBI TAC'GTA Cuts at: 493 4977 493 Size: 4484 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7186 SphI G_CATG'C Cuts at: 1132 4544 1132 Size: 3412 3774 SspI AAT'ATT Cuts at: 5 52 5110 5 Size: 47 5058 2081 Fragments arranged by size: 5058 2081 47 StuI AGG'CCT Cuts at: 1517 2321 2346 3075 3593 4406 1517 Size: 804 25 729 518 813 4297 Fragments arranged by size: 4297 813 804 729 518 25 StyI C'CwwG_G Cuts at: 513 1065 2512 2586 3076 3588 4625 513 Size: 552 1447 74 490 512 1037 3074 Fragments arranged by size: 3074 1447 1037 552 512 490 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 5213 5230 5383 5568 6013 325 Size: 1255 2208 1425 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1425 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3411 5432 7144 Size: 274 80 33 51 1443 1434 2021 1712 Cuts at: 7144 96 Size: 138 Fragments arranged by size: 2021 1712 1443 1434 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3236 3268 3477 3509 3540 3572 7146 7178 3236 Size: 32 209 32 31 32 3574 32 3244 Fragments arranged by size: 3574 3244 209 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 3055 3655 3974 1316 Size: 1739 600 319 4528 Fragments arranged by size: 4528 1739 600 319 XbaI T'CTAG_A Cuts at: 4522 4522 Size: 7186 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2198 3413 3783 3787 4306 2198 Size: 1215 370 4 519 5078 Fragments arranged by size: 5078 1215 519 370 4 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7186 XmaI C'CCGG_G Cuts at: 1870 2053 4511 1870 Size: 183 2458 4545 Fragments arranged by size: 4545 2458 183 XmnI GAAnn'nnTTC Cuts at: 2480 5315 2480 Size: 2835 4351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2410 4994 276 Size: 53 83 186 936 876 2584 2468 Fragments arranged by size: 2584 2468 936 876 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI