(Linear) (Six-base) MAPSORT of: KIEE2651.seq Check: 6456 from: 1 to: 2481 KIEE2651.orf With 182 enzymes: SgfI * November 16, 2016 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1191 2481 Size: 1191 1290 AatII G_ACGT'C Cuts at: 0 407 2481 Size: 407 2074 AccI GT'mk_AC Cuts at: 0 1493 2481 Size: 1493 988 Acc65I G'GTAC_C Cuts at: 0 1230 2481 Size: 1230 1251 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 727 898 2203 2481 Size: 286 441 171 1305 278 Fragments arranged by size: 1305 441 286 278 171 AflIII A'CryG_T Cuts at: 0 887 2252 2481 Size: 887 1365 229 Fragments arranged by size: 1365 887 229 AleI CACnn'nnGTG Cuts at: 0 730 985 2481 Size: 730 255 1496 Fragments arranged by size: 1496 730 255 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1930 1962 2481 Size: 1930 32 519 Fragments arranged by size: 1930 519 32 AlwNI CAG_nnn'CTG Cuts at: 0 414 834 2481 Size: 414 420 1647 Fragments arranged by size: 1647 420 414 ApaI G_GGCC'C Cuts at: 0 1538 2481 Size: 1538 943 AvaI C'yCGr_G Cuts at: 0 48 2341 2481 Size: 48 2293 140 Fragments arranged by size: 2293 140 48 AvrII C'CTAG_G Cuts at: 0 1071 2481 Size: 1071 1410 BamHI G'GATC_C Cuts at: 0 2142 2481 Size: 2142 339 BanI G'GyrC_C Cuts at: 0 87 1230 1504 1570 2481 Size: 87 1143 274 66 911 Fragments arranged by size: 1143 911 274 87 66 BanII G_rGCy'C Cuts at: 0 1096 1538 2111 2339 2481 Size: 1096 442 573 228 142 Fragments arranged by size: 1096 573 442 228 142 BbeI G_GCGC'C Cuts at: 0 1574 2481 Size: 1574 907 BbsI GAAGACnn'nnnn_ Cuts at: 0 1965 2481 Size: 1965 516 BbvCI CC'TCA_GC Cuts at: 0 2228 2481 Size: 2228 253 BfrBI ATG'CAT Cuts at: 0 1140 2481 Size: 1140 1341 BglII A'GATC_T Cuts at: 0 881 2481 Size: 881 1600 BlpI GC'TnA_GC Cuts at: 0 10 2481 Size: 10 2471 Bme1580I G_kGCm'C Cuts at: 0 722 1538 2481 Size: 722 816 943 Fragments arranged by size: 943 816 722 BmgBI CAC'GTC Cuts at: 0 2253 2481 Size: 2253 228 BmrI ACTGGGnnnn_n' Cuts at: 0 1644 1892 2481 Size: 1644 248 589 Fragments arranged by size: 1644 589 248 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 411 2481 Size: 411 2070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 470 2232 2326 2399 2481 Size: 470 1762 94 73 82 Fragments arranged by size: 1762 470 94 82 73 Bpu10I CC'TnA_GC Cuts at: 0 2228 2481 Size: 2228 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 1627 2029 2443 2481 Size: 730 897 402 414 38 Fragments arranged by size: 897 730 414 402 38 BsaI GGTCTCn'nnnn_ Cuts at: 0 382 587 1522 2068 2481 Size: 382 205 935 546 413 Fragments arranged by size: 935 546 413 382 205 BsaAI yAC'GTr Cuts at: 0 384 684 888 2481 Size: 384 300 204 1593 Fragments arranged by size: 1593 384 300 204 BsaHI Gr'CG_yC Cuts at: 0 404 1571 2481 Size: 404 1167 910 Fragments arranged by size: 1167 910 404 BsaWI w'CCGG_w Cuts at: 0 350 537 1058 2481 Size: 350 187 521 1423 Fragments arranged by size: 1423 521 350 187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1714 1744 2481 Size: 1714 30 737 Fragments arranged by size: 1714 737 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2017 2020 2347 2481 Size: 2017 3 327 134 Fragments arranged by size: 2017 327 134 3 BseYI C'CCAG_C Cuts at: 0 687 1163 1599 2056 2356 2481 Size: 687 476 436 457 300 125 Fragments arranged by size: 687 476 457 436 300 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 737 1015 1219 2015 2481 Size: 301 436 278 204 796 466 Fragments arranged by size: 796 466 436 301 278 204 BsiEI CG_ry'CG Cuts at: 0 716 1019 2481 Size: 716 303 1462 Fragments arranged by size: 1462 716 303 BsiHKAI G_wGCw'C Cuts at: 0 2339 2481 Size: 2339 142 Bsp1286I G_dGCh'C Cuts at: 0 722 1096 1538 2111 2339 2481 Size: 722 374 442 573 228 142 Fragments arranged by size: 722 573 442 374 228 142 BspEI T'CCGG_A Cuts at: 0 350 2481 Size: 350 2131 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 842 1191 1289 1331 2481 Size: 842 349 98 42 1150 Fragments arranged by size: 1150 842 349 98 42 BsrBI CCG'CTC Cuts at: 0 603 1698 2481 Size: 603 1095 783 Fragments arranged by size: 1095 783 603 BsrFI r'CCGG_y Cuts at: 0 738 2481 Size: 738 1743 BssSI C'ACGA_G Cuts at: 0 844 2104 2481 Size: 844 1260 377 Fragments arranged by size: 1260 844 377 BstAPI GCAn_nnn'nTGC Cuts at: 0 87 1323 2029 2481 Size: 87 1236 706 452 Fragments arranged by size: 1236 706 452 87 BstXI CCAn_nnnn'nTGG Cuts at: 0 84 252 532 1264 1567 2088 2481 Size: 84 168 280 732 303 521 393 Fragments arranged by size: 732 521 393 303 280 168 84 BstYI r'GATC_y Cuts at: 0 587 881 1427 1904 2142 2481 Size: 587 294 546 477 238 339 Fragments arranged by size: 587 546 477 339 294 238 Bsu36I CC'TnA_GG Cuts at: 0 1065 1151 2481 Size: 1065 86 1330 Fragments arranged by size: 1330 1065 86 BtgI C'CryG_G Cuts at: 0 1097 2481 Size: 1097 1384 BtsI GCAGTG_nn' Cuts at: 0 2027 2222 2481 Size: 2027 195 259 Fragments arranged by size: 2027 259 195 DraIII CAC_nnn'GTG Cuts at: 0 1089 2481 Size: 1089 1392 EaeI y'GGCC_r Cuts at: 0 198 578 713 817 1016 1100 1706 1895 Size: 198 380 135 104 199 84 606 189 Cuts at: 1895 2481 Size: 586 Fragments arranged by size: 606 586 380 199 198 189 135 104 84 EagI C'GGCC_G Cuts at: 0 713 1016 2481 Size: 713 303 1465 Fragments arranged by size: 1465 713 303 EarI CTCTTCn'nnn_ Cuts at: 0 466 1039 1928 2481 Size: 466 573 889 553 Fragments arranged by size: 889 573 553 466 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2203 2481 Size: 2203 278 EcoICRI GAG'CTC Cuts at: 0 2337 2481 Size: 2337 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 470 727 898 2203 2232 2326 2399 Size: 286 184 257 171 1305 29 94 73 Cuts at: 2399 2481 Size: 82 Fragments arranged by size: 1305 286 257 184 171 94 82 73 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 865 2481 Size: 865 1616 EcoO109I rG'GnC_Cy Cuts at: 0 831 873 1434 1535 1596 1803 2178 2481 Size: 831 42 561 101 61 207 375 303 Fragments arranged by size: 831 561 375 303 207 101 61 42 HaeII r_GCGC'y Cuts at: 0 17 1574 1890 2481 Size: 17 1557 316 591 Fragments arranged by size: 1557 591 316 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1117 1149 1714 1746 2481 Size: 1117 32 565 32 735 Fragments arranged by size: 1117 735 565 32 32 HindIII A'AGCT_T Cuts at: 0 114 514 2481 Size: 114 400 1967 Fragments arranged by size: 1967 400 114 KasI G'GCGC_C Cuts at: 0 1570 2481 Size: 1570 911 KpnI G_GTAC'C Cuts at: 0 1234 2481 Size: 1234 1247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1079 1898 2481 Size: 1079 819 583 Fragments arranged by size: 1079 819 583 MscI TGG'CCA Cuts at: 0 200 580 1102 1708 1897 2481 Size: 200 380 522 606 189 584 Fragments arranged by size: 606 584 522 380 200 189 MslI CAynn'nnrTG Cuts at: 0 82 250 550 730 985 1135 1262 2086 Size: 82 168 300 180 255 150 127 824 Cuts at: 2086 2089 2481 Size: 3 392 Fragments arranged by size: 824 392 300 255 180 168 150 127 82 3 MspA1I CmG'CkG Cuts at: 0 232 2060 2419 2481 Size: 232 1828 359 62 Fragments arranged by size: 1828 359 232 62 NaeI GCC'GGC Cuts at: 0 740 2481 Size: 740 1741 NarI GG'CG_CC Cuts at: 0 1571 2481 Size: 1571 910 NgoMIV G'CCGG_C Cuts at: 0 738 2481 Size: 738 1743 NsiI A_TGCA'T Cuts at: 0 1142 2481 Size: 1142 1339 NspI r_CATG'y Cuts at: 0 424 1144 1996 2481 Size: 424 720 852 485 Fragments arranged by size: 852 720 485 424 PasI CC'CwG_GG Cuts at: 0 1791 2481 Size: 1791 690 PflMI CCAn_nnn'nTGG Cuts at: 0 687 1089 1781 2481 Size: 687 402 692 700 Fragments arranged by size: 700 692 687 402 PfoI T'CCnGG_A Cuts at: 0 466 500 860 1394 1732 2481 Size: 466 34 360 534 338 749 Fragments arranged by size: 749 534 466 360 338 34 PmlI CAC'GTG Cuts at: 0 384 684 2481 Size: 384 300 1797 Fragments arranged by size: 1797 384 300 PpuMI rG'GwC_Cy Cuts at: 0 831 1434 1596 2178 2481 Size: 831 603 162 582 303 Fragments arranged by size: 831 603 582 303 162 PspOMI G'GGCC_C Cuts at: 0 1534 2481 Size: 1534 947 PstI C_TGCA'G Cuts at: 0 59 2481 Size: 59 2422 PvuII CAG'CTG Cuts at: 0 232 2419 2481 Size: 232 2187 62 Fragments arranged by size: 2187 232 62 SacI G_AGCT'C Cuts at: 0 2339 2481 Size: 2339 142 SanDI GG'GwC_CC Cuts at: 0 1434 2481 Size: 1434 1047 SapI GCTCTTCn'nnn_ Cuts at: 0 1039 2481 Size: 1039 1442 SexAI A'CCwGG_T Cuts at: 0 1841 1967 2481 Size: 1841 126 514 Fragments arranged by size: 1841 514 126 SfcI C'TryA_G Cuts at: 0 55 1828 2327 2481 Size: 55 1773 499 154 Fragments arranged by size: 1773 499 154 55 SfoI GGC'GCC Cuts at: 0 1572 2481 Size: 1572 909 SmaI CCC'GGG Cuts at: 0 50 2481 Size: 50 2431 SmlI C'TyrA_G Cuts at: 0 745 1642 2044 2458 2481 Size: 745 897 402 414 23 Fragments arranged by size: 897 745 414 402 23 StuI AGG'CCT Cuts at: 0 316 341 1070 1588 2401 2481 Size: 316 25 729 518 813 80 Fragments arranged by size: 813 729 518 316 80 25 StyI C'CwwG_G Cuts at: 0 507 581 1071 1583 2481 Size: 507 74 490 512 898 Fragments arranged by size: 898 512 507 490 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1783 2481 Size: 1783 698 TatI w'GTAC_w Cuts at: 0 1406 2481 Size: 1406 1075 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1231 1263 1472 1504 1535 1567 2481 Size: 1231 32 209 32 31 32 914 Fragments arranged by size: 1231 914 209 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 0 1050 1650 1969 2481 Size: 1050 600 319 512 Fragments arranged by size: 1050 600 512 319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 193 1408 1778 1782 2301 2481 Size: 193 1215 370 4 519 180 Fragments arranged by size: 1215 519 370 193 180 4 XmaI C'CCGG_G Cuts at: 0 48 2481 Size: 48 2433 XmnI GAAnn'nnTTC Cuts at: 0 475 2481 Size: 475 2006 ZraI GAC'GTC Cuts at: 0 405 2481 Size: 405 2076 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflIII AleI AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI BaeI BcgI BciVI BclI BglI BmtI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI MfeI MluI NcoI NdeI NheI NotI NruI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI