Restriction map for
pFN21AE5149
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# NO CUT ENZYMES ( 197 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
Fnu4HI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI ItaI
KasI KpnI KspI MaeI MamI
MfeI MluI MseI MslI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I TseI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 65 TCCGGA T^CCGGA
AluI 150 AGCT AG^CT
AlwNI 188 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 44 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanI 199 GGTGCC G^G[CT][GA]CC
BsaWI 65 TCCGGA [AT]^CCGG[AT]
BseAI 65 TCCGGA T^CCGGA
BsmAI 144 GAGAC GTCTC(1/5)
BsmBI 144 GAGACG CGTCTC(1/5)
BsmFI 43 GGGAC GGGAC(10/14)
BspEI 65 TCCGGA T^CCGGA
BssKI 203 CCTGG ^CC[ACGT]GG
BstNI 203 CCTGG CC^[AT]GG
BstYI 158 GGATCT [GA]^GATC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 141 GAAGAG CTCTTC(1/4)
EcoRII 203 CCTGG ^CC[AT]GG
FauI 47 CCCGC CCCGC(4/6)
HapII 66 CCGG C^CGG
HpaII 66 CCGG C^CGG
HphI 75 GGTGA GGTGA(8/7)
Ksp632I 141 GAAGAG CTCTTC(1/4)
MaeIII 229 GTCAC ^GT[ACGT]AC
MflI 158 GGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MroI 65 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MspA1I 224 CCGCTG C[AC]G^C[GT]G
MspI 66 CCGG C^CGG
MvaI 203 CCTGG CC^[AT]GG
MwoI 193 GCTGGCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 180 CATG CATG^
PpuMI 43 GGGACCC [GA]G^G[AT]CC[CT]
ScrFI 203 CCTGG CC^[ACGT]GG
Tsp45I 229 GTCAC ^GT[GC]AC
Tsp509I 84 AATT ^AATT
XhoII 158 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 26 GGATC GGATC(4/5)
158 GGATC
BbsI 183 GAAGAC GAAGAC(2/6)
251 GAAGAC
BpuAI 183 GAAGAC GAAGAC(2/6)
251 GAAGAC
Cfr13I 44 GGACC G^G[ACGT]CC
56 GGGCC
DpnII 27 GATC ^GATC
159 GATC
DraII 43 GGGACCC [GA]G^G[ACGT]CC[CT]
55 GGGGCCT
EcoO109I 43 GGGACCC [GA]G^G[ACGT]CC[CT]
55 GGGGCCT
HaeIII 3 GGCC GG^CC
57 GGCC
MaeII 9 ACGT A^CGT
208 ACGT
MboI 27 GATC ^GATC
159 GATC
MnlI 59 CCTC CCTC(7/6)
63 CCTC
NdeII 27 GATC ^GATC
159 GATC
Sau3AI 27 GATC ^GATC
159 GATC
Sau96I 44 GGACC G^G[ACGT]CC
56 GGGCC
TspRI 166 CACTG CA[GC]TG[ACGT][ACGT]^
188 CAGTG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AciI AfaI BsiYI BslI Cac8I
MboII NlaIV RsaI