################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BbeI BbrPI BcgI BglI BsaAI BsaHI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1916 GTCTAC GT^[AC][GT]AC AflII 1774 CTTAAG C^TTAAG AflIII 1145 ACATGT A^C[GA][CT]GT AlwNI 800 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 2189 AGCGCT AGC^GCT ApaI 101 GGGCCC GGGCC^C AvrII 382 CCTAGG C^CTAGG BalI 966 TGGCCA TGG^CCA BamHI 38 GGATCC G^GATCC BanII 101 GGGCCC G[GA]GC[CT]^C BbsI 1731 GTCTTC GAAGAC(2/6) BclI 2184 TGATCA T^GATCA BfrI 1774 CTTAAG C^TTAAG BglII 601 AGATCT A^GATCT BlnI 382 CCTAGG C^CTAGG BpuAI 1731 GTCTTC GAAGAC(2/6) BsaBI 2179 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2172 GAGACC GGTCTC(1/5) BsaWI 81 ACCGGA [AT]^CCGG[AT] BseRI 1280 GAGGAG GAGGAG(10/8) BsgI 2196 CTGCAC GTGCAG(16/14) BsiEI 123 CGACCG CG[GA][CT]^CG BsmI 1016 GCATTC GAATGC(1/-1) BspLU11I 1145 ACATGT A^CATGT BspMI 2089 GCAGGT ACCTGC(4/8) BsrBI 42 CCGCTC CCGCTC(-3/-3) BsrFI 26 ACCGGC [GA]^CCGG[CT] BstBI 332 TTCGAA TT^CGAA Cfr10I 26 ACCGGC [GA]^CCGG[CT] DrdI 980 GACTTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 74 CCATGG C^C[GA][CT]GG EaeI 966 TGGCCA [CT]^GGCC[GA] Eco47III 2189 AGCGCT AGC^GCT FbaI 2184 TGATCA T^GATCA HaeII 2189 AGCGCT [GA]GCGC^[CT] HgaI 67 GCGTC GACGC(5/10) MamI 2179 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 966 TGGCCA TGG^CCA MscI 966 TGGCCA TGG^CCA MspA1I 1996 CAGCTG C[AC]G^C[GT]G NcoI 74 CCATGG C^CATGG NdeI 536 CATATG CA^TATG NheI 1660 GCTAGC G^CTAGC NspV 332 TTCGAA TT^CGAA PvuII 1996 CAGCTG CAG^CTG SexAI 1173 ACCAGGT A^CC[AT]GGT SfuI 332 TTCGAA TT^CGAA SmlI 1774 CTTAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaII 1702 GGACC G^G[AT]CC 1966 GGTCC BbvCI 1232 CCTCAGC CCTCAGC(-5/-2) 1655 CCTCAGC BciVI 272 GGATAC GTATCC(6/5) 718 GTATCC BlpI 523 GCTCAGC GC^T[ACGT]AGC 847 GCTGAGC Bpu1102I 523 GCTCAGC GC^T[ACGT]AGC 847 GCTGAGC BsmFI 989 GTCCC GGGAC(10/14) 1229 GTCCC BstAPI 1047 GCAAACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2161 GCATACCATGC CelII 523 GCTCAGC GC^T[ACGT]AGC 847 GCTGAGC EcoRI 420 GAATTC G^AATTC 1156 GAATTC EcoT14I 74 CCATGG C^C[AT][AT]GG 382 CCTAGG MslI 346 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1094 CACCTTCATG PstI 563 CTGCAG CTGCA^G 2087 CTGCAG StyI 74 CCATGG C^C[AT][AT]GG 382 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI BanI BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII