(Circular) (Six-base) MAPSORT of: pFN21AB5790.seq Check: 6178 from: 1 to: 7036 pFN21AB5790 7036 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4085 4085 Size: 7036 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4846 278 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3922 4379 1937 Size: 1985 457 4594 Fragments arranged by size: 4594 1985 457 Acc65I G'GTAC_C Cuts at: 4357 4357 Size: 7036 AclI AA'CG_TT Cuts at: 1563 5163 5536 1563 Size: 3600 373 3063 Fragments arranged by size: 3600 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 2853 3446 3723 3987 5099 6483 6751 Size: 840 381 593 277 264 1112 1384 268 Cuts at: 6751 1632 Size: 1917 Fragments arranged by size: 1917 1384 1112 840 593 381 277 268 264 AfeI AGC'GCT Cuts at: 4196 4196 Size: 7036 AflII C'TTAA_G Cuts at: 820 1017 3779 820 Size: 197 2762 4077 Fragments arranged by size: 4077 2762 197 AflIII A'CryG_T Cuts at: 1203 3150 5935 6912 1203 Size: 1947 2785 977 1327 Fragments arranged by size: 2785 1947 1327 977 AgeI A'CCGG_T Cuts at: 5867 5867 Size: 7036 AhdI GACnn_n'nnGTC Cuts at: 5765 5765 Size: 7036 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7036 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7036 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2268 2236 Size: 32 7004 AlwNI CAG_nnn'CTG Cuts at: 2810 4779 6351 2810 Size: 1969 1572 3495 Fragments arranged by size: 3495 1969 1572 ApaI G_GGCC'C Cuts at: 2110 2110 Size: 7036 ApaLI G'TGCA_C Cuts at: 5093 6249 5093 Size: 1156 5880 ApoI r'AATT_y Cuts at: 1493 2341 2425 2546 2659 2687 3161 3899 3960 Size: 848 84 121 113 28 474 738 61 Cuts at: 3960 4345 4571 4723 6832 1493 Size: 385 226 152 2109 1697 Fragments arranged by size: 2109 1697 848 738 474 385 226 152 121 113 84 61 28 AseI AT'TA_AT Cuts at: 160 1032 5590 160 Size: 872 4558 1606 Fragments arranged by size: 4558 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 AvaI C'yCGr_G Cuts at: 1870 1943 4361 1870 Size: 73 2418 4545 Fragments arranged by size: 4545 2418 73 AvrII C'CTAG_G Cuts at: 2387 2387 Size: 7036 BamHI G'GATC_C Cuts at: 2043 4366 4733 2043 Size: 2323 367 4346 Fragments arranged by size: 4346 2323 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 3058 3420 4357 5812 Size: 325 217 627 312 959 362 937 1455 Cuts at: 5812 618 Size: 1842 Fragments arranged by size: 1842 1455 959 937 627 362 325 312 217 BanII G_rGCy'C Cuts at: 1391 2110 4355 1391 Size: 719 2245 4072 Fragments arranged by size: 4072 2245 719 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3729 928 Size: 971 1830 4235 Fragments arranged by size: 4235 1830 971 BbvCI CC'TCA_GC Cuts at: 3238 3661 3238 Size: 423 6613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5227 5261 1903 Size: 34 50 34 3206 34 3678 Fragments arranged by size: 3678 3206 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2271 2734 4928 6138 2271 Size: 463 2194 1210 3169 Fragments arranged by size: 3169 2194 1210 463 BclI T'GATC_A Cuts at: 1548 4189 1548 Size: 2641 4395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5647 136 Size: 107 122 71 631 605 3975 1525 Fragments arranged by size: 3975 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2606 7031 2606 Size: 4425 2611 BlpI GC'TnA_GC Cuts at: 2529 2853 4453 2529 Size: 324 1600 5112 Fragments arranged by size: 5112 1600 324 Bme1580I G_kGCm'C Cuts at: 1790 2110 3063 3423 5097 6253 1790 Size: 320 953 360 1674 1156 2573 Fragments arranged by size: 2573 1674 1156 953 360 320 BmgBI CAC'GTC Cuts at: 1318 4141 1318 Size: 2823 4213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3699 4767 5725 Size: 416 53 83 399 24 2282 1068 958 Cuts at: 5725 442 Size: 1753 Fragments arranged by size: 2282 1753 1068 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3669 1056 Size: 2613 4423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2737 2853 3431 3544 5696 1239 Size: 917 581 116 578 113 2152 2579 Fragments arranged by size: 2579 2152 917 581 578 116 113 Bpu10I CC'TnA_GC Cuts at: 3238 3661 3238 Size: 423 6613 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5161 6026 6324 5161 Size: 865 298 5873 Fragments arranged by size: 5873 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4171 5699 882 Size: 617 2672 1528 2219 Fragments arranged by size: 2672 2219 1528 617 BsaAI yAC'GTr Cuts at: 493 1204 4827 493 Size: 711 3623 2702 Fragments arranged by size: 3623 2702 711 BsaBI GATnn'nnATC Cuts at: 4188 7030 4188 Size: 2842 4194 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4843 5225 5860 275 Size: 53 83 186 936 3310 382 635 1451 Fragments arranged by size: 3310 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2086 5469 5867 6141 6288 6699 2086 Size: 3383 398 274 147 411 2423 Fragments arranged by size: 3383 2423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3030 3060 1178 Size: 30 165 30 1627 30 5154 Fragments arranged by size: 5154 1627 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3300 1186 Size: 2114 4922 BseYI C'CCAG_C Cuts at: 2499 6239 6735 2499 Size: 3740 496 2800 Fragments arranged by size: 3740 2800 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4186 1163 Size: 573 2450 4013 Fragments arranged by size: 4013 2450 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2131 4486 5247 6275 1540 Size: 275 186 130 2355 761 1028 2301 Fragments arranged by size: 2355 2301 1028 761 275 186 130 BsiHKAI G_wGCw'C Cuts at: 2208 2530 3032 5097 5182 6253 2208 Size: 322 502 2065 85 1071 2991 Fragments arranged by size: 2991 2065 1071 502 322 85 BsmI GAATG_Cn' Cuts at: 3021 4547 4640 3021 Size: 1526 93 5417 Fragments arranged by size: 5417 1526 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2208 2530 3032 3063 3423 4355 Size: 399 320 98 322 502 31 360 932 Cuts at: 4355 5097 5182 6253 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 932 742 502 399 360 322 320 98 85 31 BspEI T'CCGG_A Cuts at: 6699 6699 Size: 7036 BspHI T'CATG_A Cuts at: 4925 6655 4925 Size: 1730 5306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4085 4391 844 Size: 3241 306 3489 Fragments arranged by size: 3489 3241 306 BsrBI CCG'CTC Cuts at: 1394 1823 2049 4923 1394 Size: 429 226 2874 3507 Fragments arranged by size: 3507 2874 429 226 BsrDI GCAATG_nn' Cuts at: 65 1248 3077 3249 3395 5531 5705 65 Size: 1183 1829 172 146 2136 174 1396 Fragments arranged by size: 2136 1829 1396 1183 174 172 146 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 5680 5867 1700 Size: 110 221 3649 187 2869 Fragments arranged by size: 3649 2869 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7036 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7036 BssSI C'ACGA_G Cuts at: 5096 6108 5096 Size: 1012 6024 BstAPI GCAn_nnn'nTGC Cuts at: 794 3058 4172 794 Size: 2264 1114 3658 Fragments arranged by size: 3658 2264 1114 BstBI TT'CG_AA Cuts at: 2338 5840 2338 Size: 3502 3534 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7036 BstYI r'GATC_y Cuts at: 1972 2043 2606 4107 4366 4733 5118 5135 6587 Size: 71 563 1501 259 367 385 17 1452 Cuts at: 6587 6673 6685 7031 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1501 1452 563 385 367 346 259 86 71 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 513 Size: 552 1014 5470 Fragments arranged by size: 5470 1014 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6297 Fragments arranged by size: 6297 683 56 BtsI GCAGTG_nn' Cuts at: 3670 3733 4089 4552 5346 5366 3670 Size: 63 356 463 794 20 5340 Fragments arranged by size: 5340 794 463 356 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7036 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7001 DraI TTT'AAA Cuts at: 4340 4340 Size: 7036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2991 6043 809 Size: 507 1675 3052 1802 Fragments arranged by size: 3052 1802 1675 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2971 4483 5372 Size: 54 1425 50 275 18 1141 1512 889 Cuts at: 5372 7001 8 Size: 1629 43 Fragments arranged by size: 1629 1512 1425 1141 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4483 1537 Size: 275 2671 4090 Fragments arranged by size: 4090 2671 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4966 1353 Size: 3613 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 5623 5997 6143 387 Size: 1064 138 476 3558 374 146 1280 Fragments arranged by size: 3558 1280 1064 476 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2472 2737 2853 3431 3446 3544 Size: 393 524 316 265 116 578 15 98 Cuts at: 3544 3723 3987 5099 5696 6483 6751 1239 Size: 179 264 1112 597 787 268 1524 Fragments arranged by size: 1524 1112 787 597 578 524 393 316 268 265 264 179 116 98 15 EcoRI G'AATT_C Cuts at: 2425 3161 4345 4723 2425 Size: 736 1184 378 4738 Fragments arranged by size: 4738 1184 736 378 FspI TGC'GCA Cuts at: 5542 5542 Size: 7036 HaeII r_GCGC'y Cuts at: 1678 4198 6183 1678 Size: 2520 1985 2531 Fragments arranged by size: 2531 2520 1985 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3217 3249 5677 5709 5751 Size: 32 135 32 1645 32 2428 32 42 Cuts at: 5751 5783 1373 Size: 32 2626 Fragments arranged by size: 2626 2428 1645 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4380 4626 669 Size: 988 281 2442 246 3079 Fragments arranged by size: 3079 2442 988 281 246 HindIII A'AGCT_T Cuts at: 748 2333 2484 3405 748 Size: 1585 151 921 4379 Fragments arranged by size: 4379 1585 921 151 HpaI GTT'AAC Cuts at: 4626 4626 Size: 7036 KpnI G_GTAC'C Cuts at: 4361 4361 Size: 7036 MfeI C'AATT_G Cuts at: 4635 4635 Size: 7036 MluI A'CGCG_T Cuts at: 6912 6912 Size: 7036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6150 6334 1305 Size: 4845 184 2007 Fragments arranged by size: 4845 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2973 10 Size: 54 1425 343 1141 4073 Fragments arranged by size: 4073 1425 1141 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 3103 4994 5353 5512 518 Size: 648 1189 748 1891 359 159 2042 Fragments arranged by size: 2042 1891 1189 748 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4003 4452 5129 6277 6522 1606 Size: 2397 449 677 1148 245 2120 Fragments arranged by size: 2397 2120 1148 677 449 245 NcoI C'CATG_G Cuts at: 513 1065 2079 513 Size: 552 1014 5470 Fragments arranged by size: 5470 1014 552 NdeI CA'TA_TG Cuts at: 387 2542 387 Size: 2155 4881 NheI G'CTAG_C Cuts at: 1052 3665 1052 Size: 2613 4423 NotI GC'GGCC_GC Cuts at: 4483 4483 Size: 7036 NspI r_CATG'y Cuts at: 1132 1229 3154 3524 3921 4394 5939 1132 Size: 97 1925 370 397 473 1545 2229 Fragments arranged by size: 2229 1925 1545 473 397 370 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7036 PciI A'CATG_T Cuts at: 3150 5935 3150 Size: 2785 4251 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7036 PfoI T'CCnGG_A Cuts at: 2051 2051 Size: 7036 PmeI GTTT'AAAC Cuts at: 4340 4340 Size: 7036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5105 5137 6618 6650 5105 Size: 32 1481 32 5491 Fragments arranged by size: 5491 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7036 PsiI TTA'TAA Cuts at: 4606 4606 Size: 7036 PspOMI G'GGCC_C Cuts at: 2106 2106 Size: 7036 PstI C_TGCA'G Cuts at: 830 2572 4096 4388 830 Size: 1742 1524 292 3478 Fragments arranged by size: 3478 1742 1524 292 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7036 PvuII CAG'CTG Cuts at: 4003 4003 Size: 7036 SalI G'TCGA_C Cuts at: 1936 4378 1936 Size: 2442 4594 SbfI CC_TGCA'GG Cuts at: 4388 4388 Size: 7036 ScaI AGT'ACT Cuts at: 5284 6996 5284 Size: 1712 5324 SexAI A'CCwGG_T Cuts at: 3178 3178 Size: 7036 SfcI C'TryA_G Cuts at: 826 1045 2215 2568 2731 4092 4384 5519 6200 Size: 219 1170 353 163 1361 292 1135 681 Cuts at: 6200 6391 826 Size: 191 1471 Fragments arranged by size: 1471 1361 1170 1135 681 353 292 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7036 SmaI CCC'GGG Cuts at: 1872 4363 1872 Size: 2491 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3779 5140 6041 6303 820 Size: 197 926 1836 1361 901 262 1553 Fragments arranged by size: 1836 1553 1361 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4827 493 Size: 4334 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7036 SphI G_CATG'C Cuts at: 1132 4394 1132 Size: 3262 3774 SspI AAT'ATT Cuts at: 5 52 4960 5 Size: 47 4908 2081 Fragments arranged by size: 4908 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7036 StyI C'CwwG_G Cuts at: 513 1065 2079 2387 4475 513 Size: 552 1014 308 2088 3074 Fragments arranged by size: 3074 2088 1014 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4257 5063 5080 5233 5418 5863 325 Size: 1255 2677 806 17 153 185 445 1498 Fragments arranged by size: 2677 1498 1255 806 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2162 3715 3809 Size: 274 80 33 51 1443 185 1553 94 Cuts at: 3809 5282 6994 96 Size: 1473 1712 138 Fragments arranged by size: 1712 1553 1473 1443 274 185 138 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2241 2273 6996 7028 2241 Size: 32 4723 32 2249 Fragments arranged by size: 4723 2249 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7036 XbaI T'CTAG_A Cuts at: 4372 4372 Size: 7036 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7036 XmaI C'CCGG_G Cuts at: 1870 4361 1870 Size: 2491 4545 XmnI GAAnn'nnTTC Cuts at: 5165 5165 Size: 7036 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4844 276 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI