################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcsI 362 AAATTC [GA]^AATT[CT] ApoI 362 AAATTC [GA]^AATT[CT] AspHI 313 GAGCTC G[AT]GC[AT]^C AvaII 342 GGACC G^G[AT]CC BamHI 395 GGATCC G^GATCC BcnI 445 CCGGG CC^[GC]GG BglII 275 AGATCT A^GATCT BpmI 382 CTGGAG CTGGAG(16/14) BsiHKAI 313 GAGCTC G[AT]GC[AT]^C BsmAI 329 GTCTC GTCTC(1/5) BsmFI 300 GGGAC GGGAC(10/14) BsmI 52 GAATGC GAATGC(1/-1) BsrDI 201 GCAATG GCAATG(2/0) BsrI 162 CCAGT ACTGG(1/-1) BstAPI 429 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 104 CCATCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 28 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 28 AGGGCCT [GA]G^G[ACGT]CC[CT] FauI 249 CCCGC CCCGC(4/6) HapII 445 CCGG C^CGG HpaII 445 CCGG C^CGG HphI 11 TCACC GGTGA(8/7) MboII 273 GAAGA GAAGA(8/7) MslI 105 CATCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 445 CCGG C^CGG NciI 445 CCGGG CC^[GC]GG NlaIII 513 CATG CATG^ PvuII 390 CAGCTG CAG^CTG SacI 313 GAGCTC GAGCT^C ScaI 164 AGTACT AGT^ACT SfaNI 408 GATGC GCATC(5/9) SfcI 417 CTACAG C^T[GA][CT]AG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 396 GATCC GGATC(4/5) 395 GGATC AlwNI 90 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 430 CAGTATCTG BanII 313 GAGCTC G[GA]GC[CT]^C 476 GGGCTC BbvCI 213 CCTCAGC CCTCAGC(-5/-2) 438 GCTGAGG BmyI 313 GAGCTC G[AGT]GC[ACT]^C 476 GGGCTC BsiYI 250 CCGCACTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 413 CCAACTACAGG BslI 250 CCGCACTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 413 CCAACTACAGG Bsp1286I 313 GAGCTC G[AGT]GC[ACT]^C 476 GGGCTC BsrBI 228 CCGCTC CCGCTC(-3/-3) 117 GAGCGG BstNI 36 CCAGG CC^[AT]GG 153 CCAGG BstYI 275 AGATCT [GA]^GATC[CT] 395 GGATCC CfoI 141 GCGC GCG^C 260 GCGC EcoRII 36 CCAGG ^CC[AT]GG 153 CCAGG HaeII 140 AGCGCC [GA]GCGC^[CT] 259 GGCGCT HhaI 141 GCGC GCG^C 260 GCGC HinP1I 141 GCGC G^CGC 260 GCGC HinfI 220 GACTC G^A[ACGT]TC 509 GAGTC MflI 275 AGATCT [GA]^GATC[CT] 395 GGATCC MspA1I 93 CCGCTG C[AC]G^C[GT]G 390 CAGCTG MvaI 36 CCAGG CC^[AT]GG 153 CCAGG NlaIV 395 GGATCC GG[ACGT]^[ACGT]CC 477 GGCTCC PleI 220 GACTC GAGTC(4/5) 509 GAGTC PshAI 302 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 322 GACGACTGTC Tsp509I 293 AATT ^AATT 363 AATT TspRI 60 CACTG CA[GC]TG[ACGT][ACGT]^ 357 CACTG XhoII 275 AGATCT [GA]^GATC[CT] 395 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BssKI BstF5I Cfr13I DdeI DpnII Fnu4HI FokI HaeIII ItaI MboI MnlI MwoI NdeII RsaI Sau3AI Sau96I ScrFI TseI