Restriction map for
pFN21AE6528
(Circular) (Six-base) MAPSORT of: pFN21AE6528.seq Check: 5948 from: 1 to: 5230
pFN21AE6528 5230 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4677 4945 1632
Size: 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2100 2440 2973 4545 2100
Size: 340 533 1572 2785
Fragments arranged by size:
2785 1572 533 340
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2367 2539 2765 2917 5026 1493
Size: 874 172 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 226 172 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BamHI G'GATC_C
Cuts at: 2400 2560 2927 2400
Size: 160 367 4703
Fragments arranged by size:
4703 367 160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2322 2485 2549 1391
Size: 931 163 64 4072
Fragments arranged by size:
4072 931 163 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BbvCI CC'TCA_GC
Cuts at: 2219 2444 2219
Size: 225 5005
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 136
Size: 107 122 71 631 605 2169 1525
Fragments arranged by size:
2169 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2280 5225 2280
Size: 2945 2285
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3919 442
Size: 416 53 83 399 24 1544 958 1753
Fragments arranged by size:
1753 1544 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 3890 1239
Size: 1169 1482 2579
Fragments arranged by size:
2579 1482 1169
Bpu10I CC'TnA_GC
Cuts at: 2219 2444 2219
Size: 225 5005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3893 882
Size: 617 2394 2219
Fragments arranged by size:
2394 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3037 3419 4054 275
Size: 53 83 186 936 1504 382 635 1451
Fragments arranged by size:
1504 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5230
BseYI C'CCAG_C
Cuts at: 4433 4929 4433
Size: 496 4734
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4657
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 3441 4469 1540
Size: 275 186 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 3291 3376 4447 2322
Size: 969 85 1071 3105
Fragments arranged by size:
3105 1071 969 85
BsmI GAATG_Cn'
Cuts at: 2063 2741 2834 2063
Size: 678 93 4459
Fragments arranged by size:
4459 678 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2485 2549 3291 3376 4447 1391
Size: 399 532 163 64 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 163 85 64
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 844
Size: 1741 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 2235 3117 1394
Size: 429 301 111 882 3507
Fragments arranged by size:
3507 882 429 301 111
BsrDI GCAATG_nn'
Cuts at: 65 1248 2213 3725 3899 65
Size: 1183 965 1512 174 1396
Fragments arranged by size:
1512 1396 1183 965 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3874 4061 1700
Size: 110 2064 187 2869
Fragments arranged by size:
2869 2064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 794
Size: 1646 3584
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5230
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2116
Size: 5230
BstYI r'GATC_y
Cuts at: 1972 2280 2400 2560 2927 3312 3329 4781 4867
Size: 308 120 160 367 385 17 1452 86
Cuts at: 4867 4879 5225 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 308 160 120
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4491
Fragments arranged by size:
4491 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2677 3566 5195
Size: 54 1425 50 275 18 847 889 1629
Cuts at: 5195 8
Size: 43
Fragments arranged by size:
1629 1425 889 847 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 1537
Size: 275 865 4090
Fragments arranged by size:
4090 865 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3160 1353
Size: 1807 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3817 4191 4337 387
Size: 1064 138 2228 374 146 1280
Fragments arranged by size:
2228 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2320 2320
Size: 5230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3293 3890 4677 4945 1239
Size: 393 776 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 776 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2034 2034
Size: 5230
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 5230
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 2149 2268 4377 1678
Size: 471 119 2109 2531
Fragments arranged by size:
2531 2109 471 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2422 2454 3871
Size: 32 135 32 749 32 69 32 1417
Cuts at: 3871 3903 3945 3977 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 749 135 69 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2574 2820 669
Size: 988 281 636 246 3079
Fragments arranged by size:
3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4528 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3188 3547 3706 518
Size: 648 948 1074 359 159 2042
Fragments arranged by size:
2042 1074 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2397 2646 3323 4471 4716 1606
Size: 494 297 249 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 494 297 249 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2311 2331 1613
Size: 698 20 4512
Fragments arranged by size:
4512 698 20
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
PvuII CAG'CTG
Cuts at: 2397 2397
Size: 5230
SacI G_AGCT'C
Cuts at: 2322 2322
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 2171 3478 5190 2171
Size: 1307 1712 2211
Fragments arranged by size:
2211 1712 1307
SfcI C'TryA_G
Cuts at: 826 1045 2422 2578 3713 4394 4585 826
Size: 219 1377 156 1135 681 191 1471
Fragments arranged by size:
1471 1377 1135 681 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2669 513
Size: 552 939 665 3074
Fragments arranged by size:
3074 939 665 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3274 3427 3612 4057 325
Size: 1255 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 2457 3476
Size: 274 80 33 51 1443 192 288 1019
Cuts at: 3476 5188 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 288 274 192 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI