################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwI 360 GATCC GGATC(4/5) AlwNI 90 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 458 GGTGCC G^G[CT][GA]CC BbrPI 373 CACGTG CAC^GTG BpmI 379 CTGGAG CTGGAG(16/14) BsaAI 373 CACGTG [CT]AC^GT[GA] BsmFI 143 GTCCC GGGAC(10/14) BspHI 302 TCATGA T^CATGA BsrDI 201 GCAATG GCAATG(2/0) BsrI 467 ACTGG ACTGG(1/-1) BstAPI 426 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 104 CCATCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 359 AGATCC [GA]^GATC[CT] Cac8I 490 GCAGGC GC[ACGT]^[ACGT]GC DsaI 476 CCATGG C^C[GA][CT]GG HgaI 141 GCGTC GACGC(5/10) HincII 158 GTCAAC GT[CT]^[GA]AC HindII 158 GTCAAC GT[CT]^[GA]AC HinfI 220 GACTC G^A[ACGT]TC HphI 11 TCACC GGTGA(8/7) MaeII 374 ACGT A^CGT MflI 359 AGATCC [GA]^GATC[CT] MslI 105 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 93 CCGCTG C[AC]G^C[GT]G NcoI 476 CCATGG C^CATGG PflMI 372 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 220 GACTC GAGTC(4/5) PmaCI 373 CACGTG CAC^GTG PmlI 373 CACGTG CAC^GTG PpuMI 392 GGGTCCT [GA]G^G[AT]CC[CT] PstI 76 CTGCAG CTGCA^G RcaI 302 TCATGA T^CATGA SfcI 76 CTGCAG C^T[GA][CT]AG StuI 297 AGGCCT AGG^CCT TspRI 60 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 372 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 359 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaII 339 GGACC G^G[AT]CC 393 GGTCC BbsI 286 GAAGAC GAAGAC(2/6) 319 GAAGAC BcnI 108 CCGGG CC^[GC]GG 417 CCGGG BpuAI 286 GAAGAC GAAGAC(2/6) 319 GAAGAC BsmAI 239 GTCTC GTCTC(1/5) 256 GTCTC BspMI 74 ACCTGC ACCTGC(4/8) 431 ACCTGC BsrBI 228 CCGCTC CCGCTC(-3/-3) 117 GAGCGG DpnII 9 GATC ^GATC 360 GATC EcoT14I 153 CCAAGG C^C[AT][AT]GG 476 CCATGG HapII 108 CCGG C^CGG 417 CCGG HpaII 108 CCGG C^CGG 417 CCGG MboI 9 GATC ^GATC 360 GATC MspI 108 CCGG C^CGG 417 CCGG NciI 108 CCGGG CC^[GC]GG 417 CCGGG NdeII 9 GATC ^GATC 360 GATC NlaIII 303 CATG CATG^ 477 CATG Sau3AI 9 GATC ^GATC 360 GATC StyI 153 CCAAGG C^C[AT][AT]GG 476 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AfaI AluI BbvCI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII ItaI MboII MnlI MvaI MwoI NlaIV RsaI Sau96I ScrFI SfaNI TseI