(Circular) (Six-base) MAPSORT of: pFN21AE2220.seq Check: 460 from: 1 to: 5227 pFN21AE2220 5227 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3037 278 Size: 53 83 186 936 1501 2468 Fragments arranged by size: 2468 1501 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2570 1937 Size: 633 4594 Acc65I G'GTAC_C Cuts at: 2548 2548 Size: 5227 AclI AA'CG_TT Cuts at: 1563 3354 3727 1563 Size: 1791 373 3063 Fragments arranged by size: 3063 1791 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3290 4674 4942 1632 Size: 1658 1384 268 1917 Fragments arranged by size: 1917 1658 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5030 AflIII A'CryG_T Cuts at: 1203 4126 5103 1203 Size: 2923 977 1327 Fragments arranged by size: 2923 1327 977 AgeI A'CCGG_T Cuts at: 4058 4058 Size: 5227 AhdI GACnn_n'nnGTC Cuts at: 3956 3956 Size: 5227 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5227 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5227 AlwNI CAG_nnn'CTG Cuts at: 2100 2970 4542 2100 Size: 870 1572 2785 Fragments arranged by size: 2785 1572 870 ApaLI G'TGCA_C Cuts at: 3284 4440 3284 Size: 1156 4071 ApoI r'AATT_y Cuts at: 1493 2536 2762 2914 5023 1493 Size: 1043 226 152 2109 1697 Fragments arranged by size: 2109 1697 1043 226 152 AseI AT'TA_AT Cuts at: 160 1032 3781 160 Size: 872 2749 1606 Fragments arranged by size: 2749 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5227 AvaI C'yCGr_G Cuts at: 1870 1943 2552 1870 Size: 73 609 4545 Fragments arranged by size: 4545 609 73 BamHI G'GATC_C Cuts at: 2557 2924 2557 Size: 367 4860 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2463 2548 4003 618 Size: 325 217 627 676 85 1455 1842 Fragments arranged by size: 1842 1455 676 627 325 217 85 BanII G_rGCy'C Cuts at: 1391 2546 1391 Size: 1155 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2298 2331 928 Size: 971 399 33 3824 Fragments arranged by size: 3824 971 399 33 BbvCI CC'TCA_GC Cuts at: 2219 2357 2441 2219 Size: 138 84 5005 Fragments arranged by size: 5005 138 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3418 3452 1903 Size: 34 1481 34 3678 Fragments arranged by size: 3678 1481 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3119 4329 3119 Size: 1210 4017 BclI T'GATC_A Cuts at: 1548 1548 Size: 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3838 136 Size: 107 122 71 631 605 2166 1525 Fragments arranged by size: 2166 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5222 5222 Size: 5227 BlpI GC'TnA_GC Cuts at: 2644 2644 Size: 5227 Bme1580I G_kGCm'C Cuts at: 1790 3288 4444 1790 Size: 1498 1156 2573 Fragments arranged by size: 2573 1498 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2482 2958 3916 Size: 416 53 83 399 24 1065 476 958 Cuts at: 3916 442 Size: 1753 Fragments arranged by size: 1753 1065 958 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5227 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 3887 1239 Size: 1166 1482 2579 Fragments arranged by size: 2579 1482 1166 Bpu10I CC'TnA_GC Cuts at: 2219 2357 2441 2490 2219 Size: 138 84 49 4956 Fragments arranged by size: 4956 138 84 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3352 4217 4515 3352 Size: 865 298 4064 Fragments arranged by size: 4064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3890 882 Size: 617 2391 2219 Fragments arranged by size: 2391 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2380 3018 493 Size: 711 1176 638 2702 Fragments arranged by size: 2702 1176 711 638 BsaBI GATnn'nnATC Cuts at: 5221 5221 Size: 5227 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3034 3416 4051 275 Size: 53 83 186 936 1501 382 635 1451 Fragments arranged by size: 1501 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3660 4058 4332 4479 4890 3660 Size: 398 274 147 411 3997 Fragments arranged by size: 3997 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5002 Fragments arranged by size: 5002 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5227 BseYI C'CCAG_C Cuts at: 2333 2394 4430 4926 2333 Size: 61 2036 496 2634 Fragments arranged by size: 2634 2036 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4654 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2677 3438 4466 1540 Size: 275 186 676 761 1028 2301 Fragments arranged by size: 2301 1028 761 676 275 186 BsiHKAI G_wGCw'C Cuts at: 3288 3373 4444 3288 Size: 85 1071 4071 Fragments arranged by size: 4071 1071 85 BsmI GAATG_Cn' Cuts at: 2738 2831 2738 Size: 93 5134 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2546 3288 3373 4444 1391 Size: 399 756 742 85 1071 2174 Fragments arranged by size: 2174 1071 756 742 399 85 BspEI T'CCGG_A Cuts at: 4890 4890 Size: 5227 BspHI T'CATG_A Cuts at: 2307 3116 4846 2307 Size: 809 1730 2688 Fragments arranged by size: 2688 1730 809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2445 2582 844 Size: 1244 357 137 3489 Fragments arranged by size: 3489 1244 357 137 BsrBI CCG'CTC Cuts at: 1394 1823 2124 2235 3114 1394 Size: 429 301 111 879 3507 Fragments arranged by size: 3507 879 429 301 111 BsrDI GCAATG_nn' Cuts at: 65 1248 2213 3722 3896 65 Size: 1183 965 1509 174 1396 Fragments arranged by size: 1509 1396 1183 965 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3871 4058 1700 Size: 110 2061 187 2869 Fragments arranged by size: 2869 2061 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5227 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5227 BssSI C'ACGA_G Cuts at: 3287 4299 3287 Size: 1012 4215 BstAPI GCAn_nnn'nTGC Cuts at: 794 2437 794 Size: 1643 3584 BstBI TT'CG_AA Cuts at: 4031 4031 Size: 5227 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5227 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2116 Size: 5227 BstYI r'GATC_y Cuts at: 1972 2364 2557 2924 3309 3326 4778 4864 4876 Size: 392 193 367 385 17 1452 86 12 Cuts at: 4876 5222 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 392 385 367 346 193 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2481 513 Size: 552 939 477 3259 Fragments arranged by size: 3259 939 552 477 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4488 Fragments arranged by size: 4488 683 56 BtsI GCAGTG_nn' Cuts at: 2743 3537 3557 2743 Size: 794 20 4413 Fragments arranged by size: 4413 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5227 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5192 DraI TTT'AAA Cuts at: 2531 2531 Size: 5227 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4234 809 Size: 507 2918 1802 Fragments arranged by size: 2918 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2674 3563 5192 Size: 54 1425 50 275 18 844 889 1629 Cuts at: 5192 8 Size: 43 Fragments arranged by size: 1629 1425 889 844 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2674 1537 Size: 275 862 4090 Fragments arranged by size: 4090 862 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3157 1353 Size: 1804 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3814 4188 4334 387 Size: 1064 138 2225 374 146 1280 Fragments arranged by size: 2225 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 3290 3887 4674 4942 1239 Size: 393 773 885 597 787 268 1524 Fragments arranged by size: 1524 885 787 773 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2034 2398 2445 2034 Size: 364 47 4816 Fragments arranged by size: 4816 364 47 EcoRI G'AATT_C Cuts at: 2536 2914 2536 Size: 378 4849 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2035 2035 Size: 5227 FspI TGC'GCA Cuts at: 3733 3733 Size: 5227 HaeII r_GCGC'y Cuts at: 1678 4374 1678 Size: 2696 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3868 3900 3942 3974 1373 Size: 32 135 32 2296 32 42 32 2626 Fragments arranged by size: 2626 2296 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2165 2571 2817 669 Size: 988 281 227 406 246 3079 Fragments arranged by size: 3079 988 406 281 246 227 HindIII A'AGCT_T Cuts at: 748 748 Size: 5227 HpaI GTT'AAC Cuts at: 2817 2817 Size: 5227 KpnI G_GTAC'C Cuts at: 2552 2552 Size: 5227 MfeI C'AATT_G Cuts at: 2826 2826 Size: 5227 MluI A'CGCG_T Cuts at: 5103 5103 Size: 5227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4341 4525 1305 Size: 3036 184 2007 Fragments arranged by size: 3036 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3405 Fragments arranged by size: 3405 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 3185 3544 3703 518 Size: 648 948 1071 359 159 2042 Fragments arranged by size: 2042 1071 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2643 3320 4468 4713 1606 Size: 494 543 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 494 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2481 513 Size: 552 939 477 3259 Fragments arranged by size: 3259 939 552 477 NdeI CA'TA_TG Cuts at: 387 387 Size: 5227 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5227 NotI GC'GGCC_GC Cuts at: 2674 2674 Size: 5227 NspI r_CATG'y Cuts at: 1132 1229 2585 4130 1132 Size: 97 1356 1545 2229 Fragments arranged by size: 2229 1545 1356 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5227 PciI A'CATG_T Cuts at: 4126 4126 Size: 5227 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 1108 Size: 1275 3952 PfoI T'CCnGG_A Cuts at: 2340 2340 Size: 5227 PmeI GTTT'AAAC Cuts at: 2531 2531 Size: 5227 PmlI CAC'GTG Cuts at: 2380 2380 Size: 5227 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3296 3328 4809 4841 3296 Size: 32 1481 32 3682 Fragments arranged by size: 3682 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2398 2398 Size: 5227 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5227 PsiI TTA'TAA Cuts at: 2797 2797 Size: 5227 PstI C_TGCA'G Cuts at: 830 2085 2579 830 Size: 1255 494 3478 Fragments arranged by size: 3478 1255 494 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5227 SalI G'TCGA_C Cuts at: 1936 2569 1936 Size: 633 4594 SbfI CC_TGCA'GG Cuts at: 2579 2579 Size: 5227 ScaI AGT'ACT Cuts at: 3475 5187 3475 Size: 1712 3515 SfcI C'TryA_G Cuts at: 826 1045 2081 2575 3710 4391 4582 826 Size: 219 1036 494 1135 681 191 1471 Fragments arranged by size: 1471 1135 1036 681 494 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5227 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5227 SmaI CCC'GGG Cuts at: 1872 2554 1872 Size: 682 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3331 4232 4494 820 Size: 197 926 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3018 493 Size: 2525 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5227 SphI G_CATG'C Cuts at: 1132 2585 1132 Size: 1453 3774 SspI AAT'ATT Cuts at: 5 52 3151 5 Size: 47 3099 2081 Fragments arranged by size: 3099 2081 47 StuI AGG'CCT Cuts at: 1517 2304 1517 Size: 787 4440 StyI C'CwwG_G Cuts at: 513 1065 2004 2158 2481 2666 513 Size: 552 939 154 323 185 3074 Fragments arranged by size: 3074 939 552 323 185 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3254 3271 3424 3609 4054 325 Size: 1255 1674 17 153 185 445 1498 Fragments arranged by size: 1674 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2454 3473 5185 Size: 274 80 33 51 1443 477 1019 1712 Cuts at: 5185 96 Size: 138 Fragments arranged by size: 1712 1443 1019 477 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5187 5219 5187 Size: 32 5195 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5227 XbaI T'CTAG_A Cuts at: 2563 2563 Size: 5227 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5227 XmaI C'CCGG_G Cuts at: 1870 2552 1870 Size: 682 4545 XmnI GAAnn'nnTTC Cuts at: 3356 3356 Size: 5227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3035 276 Size: 53 83 186 936 1501 2468 Fragments arranged by size: 2468 1501 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI