################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AluI 404 AGCT AG^CT AlwI 266 GGATC GGATC(4/5) AspI 220 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 153 CTCGGG C^[CT]CG[GA]G BbsI 332 GTCTTC GAAGAC(2/6) BbvCI 213 CCTCAGC CCTCAGC(-5/-2) BcnI 27 CCGGG CC^[GC]GG BpuAI 332 GTCTTC GAAGAC(2/6) BseRI 501 GAGGAG GAGGAG(10/8) BsmFI 446 GGGAC GGGAC(10/14) BsoBI 153 CTCGGG C^[CT]CG[GA]G BsrBI 228 CCGCTC CCGCTC(-3/-3) BssHII 106 GCGCGC G^CGCGC BstAPI 426 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 106 GCGCGC GC[ACGT]^[ACGT]GC CpoI 224 CGGTCCG CG^G[AT]CCG DraII 392 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 177 CCGCGG C^C[GA][CT]GG EcoNI 15 CCTCTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 392 GGGTCCT [GA]G^G[ACGT]CC[CT] HaeII 455 GGCGCT [GA]GCGC^[CT] HapII 27 CCGG C^CGG HpaII 27 CCGG C^CGG HphI 11 TCACC GGTGA(8/7) KspI 177 CCGCGG CCGC^GG MaeII 374 ACGT A^CGT MspI 27 CCGG C^CGG NciI 27 CCGGG CC^[GC]GG PflFI 220 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 392 GGGTCCT [GA]G^G[AT]CC[CT] PstI 76 CTGCAG CTGCA^G RsrII 224 CGGTCCG CG^G[AT]CCG SacII 177 CCGCGG CCGC^GG ScaI 164 AGTACT AGT^ACT SmlI 87 CTTGAG C^T[CT][GA]AG Tth111I 220 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 165 GTAC GT^AC 429 GTAC AlwNI 117 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 427 CAGTACCTG BanII 384 GGGCTC G[GA]GC[CT]^C 492 GGGCTC BmyI 384 GGGCTC G[AGT]GC[ACT]^C 492 GGGCTC BpmI 160 CTCCAG CTGGAG(16/14) 379 CTGGAG BsmI 52 GAATGC GAATGC(1/-1) 480 GAATGC Bsp1286I 384 GGGCTC G[AGT]GC[ACT]^C 492 GGGCTC BspMI 74 ACCTGC ACCTGC(4/8) 431 ACCTGC BsrI 467 ACTGG ACTGG(1/-1) 162 CCAGT DpnII 9 GATC ^GATC 267 GATC MboI 9 GATC ^GATC 267 GATC MboII 6 GAAGA GAAGA(8/7) 333 TCTTC NdeII 9 GATC ^GATC 267 GATC RsaI 165 GTAC GT^AC 429 GTAC Sau3AI 9 GATC ^GATC 267 GATC SfaNI 129 GATGC GCATC(5/9) 438 GATGC TspRI 202 CAGTG CA[GC]TG[ACGT][ACGT]^ 315 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AccII AciI AvaII BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MnlI MspA1I MvaI MvnI MwoI NlaIV PleI Sau96I ScrFI SfcI TseI