################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstEII BstPI BstXI CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 527 GTATAC GT^[AC][GT]AC AccII 973 CGCG CG^CG AciI 766 GCGG CCGC(-3/-1) AcsI 1349 AAATTC [GA]^AATT[CT] AocI 256 CCTAAGG CC^T[ACGT]AGG ApoI 1349 AAATTC [GA]^AATT[CT] Asp700I 1603 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2120 TGGCCA TGG^CCA BbvCI 2059 CCTCAGC CCTCAGC(-5/-2) BcgI 174 GCAGATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 8 GGATAC GTATCC(6/5) BlpI 1807 GCTCAGC GC^T[ACGT]AGC BpmI 800 CTGGAG CTGGAG(16/14) Bpu1102I 1807 GCTCAGC GC^T[ACGT]AGC BsaAI 824 CACGTA [CT]AC^GT[GA] BsgI 1436 CTGCAC GTGCAG(16/14) BsiEI 140 CGACCG CG[GA][CT]^CG BspMI 1024 GCAGGT ACCTGC(4/8) Bst1107I 527 GTATAC GTA^TAC BstUI 973 CGCG CG^CG BstZ17I 527 GTATAC GTA^TAC Bsu36I 256 CCTAAGG CC^T[ACGT]AGG CelII 1807 GCTCAGC GC^T[ACGT]AGC DraII 1391 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 2120 TGGCCA [CT]^GGCC[GA] Eco81I 256 CCTAAGG CC^T[ACGT]AGG EcoO109I 1391 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 670 GATATC GAT^ATC EcoT22I 829 ATGCAT ATGCA^T HincII 1789 GTCAAC GT[CT]^[GA]AC HindII 1789 GTCAAC GT[CT]^[GA]AC HindIII 152 AAGCTT A^AGCTT MluNI 2120 TGGCCA TGG^CCA MscI 2120 TGGCCA TGG^CCA MspA1I 1472 CAGCTG C[AC]G^C[GT]G MvnI 973 CGCG CG^CG NsiI 829 ATGCAT ATGCA^T NspI 170 GCATGC [GA]CATG^[CT] PpuMI 1391 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 1472 CAGCTG CAG^CTG SpeI 1507 ACTAGT A^CTAGT SphI 170 GCATGC GCATG^C SspI 778 AATATT AAT^ATT XmnI 1603 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 536 GTAC GT^AC 1211 GTAC AlwNI 1124 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1754 CAGGAGCTG BcnI 133 CCGGG CC^[GC]GG 719 CCCGG BspHI 195 TCATGA T^CATGA 515 TCATGA BsrDI 1362 CATTGC GCAATG(2/0) 1485 CATTGC BstBI 62 TTCGAA TT^CGAA 1253 TTCGAA BstYI 176 AGATCC [GA]^GATC[CT] 854 GGATCT HapII 133 CCGG C^CGG 720 CCGG HpaII 133 CCGG C^CGG 720 CCGG MfeI 1019 CAATTG C^AATTG 1169 CAATTG MflI 176 AGATCC [GA]^GATC[CT] 854 GGATCT MspI 133 CCGG C^CGG 720 CCGG MunI 1019 CAATTG C^AATTG 1169 CAATTG NciI 133 CCGGG CC^[GC]GG 719 CCCGG NspV 62 TTCGAA TT^CGAA 1253 TTCGAA RcaI 195 TCATGA T^CATGA 515 TCATGA RsaI 536 GTAC GT^AC 1211 GTAC SacI 1805 GAGCTC GAGCT^C 2008 GAGCTC SfuI 62 TTCGAA TT^CGAA 1253 TTCGAA StuI 23 AGGCCT AGG^CCT 33 AGGCCT XcmI 749 CCATTGATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1443 CCAAAGAAATGATGG XhoII 176 AGATCC [GA]^GATC[CT] 854 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AluI AlwI AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I