################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 922 GTAGAC GT^[AC][GT]AC AclI 160 AACGTT AA^CGTT AlwI 1057 GATCC GGATC(4/5) AspHI 175 GAGCTC G[AT]GC[AT]^C BanI 795 GGTGCC G^G[CT][GA]CC BanII 175 GAGCTC G[GA]GC[CT]^C BciVI 77 GTATCC GTATCC(6/5) BmyI 175 GAGCTC G[AGT]GC[ACT]^C BsiHKAI 175 GAGCTC G[AT]GC[AT]^C Bsp1286I 175 GAGCTC G[AGT]GC[ACT]^C BspMI 792 GCAGGT ACCTGC(4/8) BstYI 1056 AGATCC [GA]^GATC[CT] DpnII 1057 GATC ^GATC EarI 194 GAAGAG CTCTTC(1/4) EcoRI 1249 GAATTC G^AATTC EcoRV 868 GATATC GAT^ATC EcoT14I 1198 CCTTGG C^C[AT][AT]GG EcoT22I 811 ATGCAT ATGCA^T HapII 342 CCGG C^CGG HincII 434 GTCAAC GT[CT]^[GA]AC HindII 434 GTCAAC GT[CT]^[GA]AC HindIII 305 AAGCTT A^AGCTT HpaII 342 CCGG C^CGG HphI 580 GGTGA GGTGA(8/7) Ksp632I 194 GAAGAG CTCTTC(1/4) MboI 1057 GATC ^GATC MflI 1056 AGATCC [GA]^GATC[CT] MspA1I 387 CAGCTG C[AC]G^C[GT]G MspI 342 CCGG C^CGG NdeII 1057 GATC ^GATC NsiI 811 ATGCAT ATGCA^T PshAI 213 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 160 AACGTT AA^CGTT PvuII 387 CAGCTG CAG^CTG SacI 175 GAGCTC GAGCT^C Sau3AI 1057 GATC ^GATC ScaI 144 AGTACT AGT^ACT SmlI 441 CTTGAG C^T[CT][GA]AG SspI 352 AATATT AAT^ATT StyI 1198 CCTTGG C^C[AT][AT]GG XhoII 1056 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 906 AAATTC [GA]^AATT[CT] 1249 GAATTC AfaI 145 GTAC GT^AC 1036 GTAC AlwNI 823 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 835 CAGAAACTG ApoI 906 AAATTC [GA]^AATT[CT] 1249 GAATTC AvaII 446 GGACC G^G[AT]CC 847 GGTCC BbvI 386 GCAGC GCAGC(8/12) 687 GCAGC BfaI 131 CTAG C^TAG 589 CTAG BpmI 954 CTCCAG CTGGAG(16/14) 1187 CTCCAG BsiYI 107 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 503 CCGTGAGAGGG BslI 107 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 503 CCGTGAGAGGG BsmFI 609 GTCCC GGGAC(10/14) 1092 GTCCC BsrDI 1181 CATTGC GCAATG(2/0) 287 GCAATG Cfr13I 446 GGACC G^G[ACGT]CC 847 GGTCC DraII 445 AGGACCT [GA]G^G[ACGT]CC[CT] 846 AGGTCCT DsaI 242 CCACGG C^C[GA][CT]GG 691 CCGTGG EcoO109I 445 AGGACCT [GA]G^G[ACGT]CC[CT] 846 AGGTCCT Fnu4HI 386 GCAGC GC^[ACGT]GC 687 GCAGC ItaI 386 GCAGC GC^[ACGT]GC 687 GCAGC MaeI 131 CTAG C^TAG 589 CTAG MaeII 120 ACGT A^CGT 161 ACGT MslI 526 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 678 CACATCAGTG MwoI 687 GCAGCCGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1041 GCTCTGTTTGC NlaIV 499 GGAGCC GG[ACGT]^[ACGT]CC 795 GGTGCC NspI 772 GCATGT [GA]CATG^[CT] 809 ACATGC PpuMI 445 AGGACCT [GA]G^G[AT]CC[CT] 846 AGGTCCT RsaI 145 GTAC GT^AC 1036 GTAC Sau96I 446 GGACC G^G[ACGT]CC 847 GGTCC SfaNI 825 GATGC GCATC(5/9) 345 GCATC TseI 386 GCAGC G^C[AT]GC 687 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI BsaJI BsgI BsmAI BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII FokI HaeIII HinfI MaeIII MboII MnlI MseI MvaI NlaIII PleI ScrFI TfiI Tru9I Tsp45I Tsp509I TspRI XcmI