(Circular) (Six-base) MAPSORT of: pFN21AB6641.seq Check: 7684 from: 1 to: 5959 pFN21AB6641 5959 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2788 2788 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2928 3302 1937 Size: 991 374 4594 Fragments arranged by size: 4594 991 374 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 2166 4086 4459 1563 Size: 603 1920 373 3063 Fragments arranged by size: 3063 1920 603 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2484 4022 5406 5674 1632 Size: 852 1538 1384 268 1917 Fragments arranged by size: 1917 1538 1384 852 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 2687 1166 Size: 1521 4438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2705 1184 Size: 1521 4438 AlwNI CAG_nnn'CTG Cuts at: 2833 2845 3702 5274 2833 Size: 12 857 1572 3518 Fragments arranged by size: 3518 1572 857 12 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2911 3254 3268 3494 3646 5755 1493 Size: 1418 343 14 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 343 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2800 3280 4735 618 Size: 325 217 627 1013 480 1455 1842 Fragments arranged by size: 1842 1455 1013 627 480 325 217 BanII G_rGCy'C Cuts at: 1391 2184 3278 1391 Size: 793 1094 4072 Fragments arranged by size: 4072 1094 793 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2093 3851 5061 2093 Size: 1758 1210 2991 Fragments arranged by size: 2991 1758 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BfrBI ATG'CAT Cuts at: 2818 2818 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 2126 1318 Size: 808 5151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2911 3259 3690 Size: 416 53 83 399 24 1494 348 431 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 1494 958 431 416 399 348 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2944 3177 4619 1239 Size: 1705 233 1442 2579 Fragments arranged by size: 2579 1705 1442 233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2467 4084 4949 5247 2467 Size: 1617 865 298 3179 Fragments arranged by size: 3179 1617 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2934 2964 3004 3034 1178 Size: 30 165 30 1531 30 40 30 4103 Fragments arranged by size: 4103 1531 165 40 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5959 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 2513 2711 1163 Size: 573 275 502 198 4411 Fragments arranged by size: 4411 573 502 275 198 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 4020 4105 5176 2184 Size: 1836 85 1071 2967 Fragments arranged by size: 2967 1836 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 3278 4020 4105 5176 1391 Size: 399 394 1094 742 85 1071 2174 Fragments arranged by size: 2174 1094 1071 742 399 394 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2788 3314 844 Size: 1944 526 3489 Fragments arranged by size: 3489 1944 526 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2299 3185 4454 4628 65 Size: 1183 1051 886 1269 174 1396 Fragments arranged by size: 1396 1269 1183 1051 886 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3061 3289 3656 4041 4058 5510 5596 5608 Size: 1089 228 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 228 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2247 2696 513 Size: 552 1182 449 3776 Fragments arranged by size: 3776 1182 552 449 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924 Size: 54 1425 50 275 18 1576 889 1629 Cuts at: 5924 8 Size: 43 Fragments arranged by size: 1629 1576 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2194 3889 1353 Size: 841 1695 3423 Fragments arranged by size: 3423 1695 841 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2182 2182 Size: 5959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2484 2944 3177 4022 4619 5406 5674 Size: 393 852 460 233 845 597 787 268 Cuts at: 5674 1239 Size: 1524 Fragments arranged by size: 1524 852 845 787 597 460 393 268 233 EcoO109I rG'GnC_Cy Cuts at: 2451 2852 2451 Size: 401 5558 EcoRI G'AATT_C Cuts at: 3254 3268 3646 3254 Size: 14 378 5567 Fragments arranged by size: 5567 378 14 EcoRV GAT'ATC Cuts at: 2875 2875 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373 Size: 32 135 32 3028 32 42 32 2626 Fragments arranged by size: 3028 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2441 3303 3549 669 Size: 988 281 503 862 246 3079 Fragments arranged by size: 3079 988 862 503 281 246 HindIII A'AGCT_T Cuts at: 748 2310 748 Size: 1562 4397 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2535 2687 3917 4276 4435 518 Size: 648 1369 152 1230 359 159 2042 Fragments arranged by size: 2042 1369 1230 648 359 159 152 MspA1I CmG'CkG Cuts at: 1606 2394 3375 4052 5200 5445 1606 Size: 788 981 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 788 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NsiI A_TGCA'T Cuts at: 2820 2820 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2781 2818 3317 4862 1132 Size: 97 1552 37 499 1545 2229 Fragments arranged by size: 2229 1552 1545 499 97 37 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2808 2808 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2451 2852 2451 Size: 401 5558 PshAI GACnn'nnGTC Cuts at: 1613 2222 1613 Size: 609 5350 PsiI TTA'TAA Cuts at: 3168 3529 3168 Size: 361 5598 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2394 2394 Size: 5959 SacI G_AGCT'C Cuts at: 2184 2184 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 2151 4207 5919 2151 Size: 2056 1712 2191 Fragments arranged by size: 2191 2056 1712 SfcI C'TryA_G Cuts at: 826 1045 3307 4442 5123 5314 826 Size: 219 2262 1135 681 191 1471 Fragments arranged by size: 2262 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2446 4063 4964 5226 820 Size: 197 926 503 1617 901 262 1553 Fragments arranged by size: 1617 1553 926 901 503 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 2359 3883 5 Size: 47 2307 1524 2081 Fragments arranged by size: 2307 2081 1524 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 3203 3398 513 Size: 552 2138 195 3074 Fragments arranged by size: 3074 2138 552 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3003 3986 4003 4156 4341 4786 325 Size: 1255 1423 983 17 153 185 445 1498 Fragments arranged by size: 1498 1423 1255 983 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2149 4205 5917 Size: 274 80 33 51 1443 172 2056 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 2056 1712 1443 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2934 2966 2987 3019 5919 5951 2934 Size: 32 21 32 2900 32 2942 Fragments arranged by size: 2942 2900 32 32 32 21 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2115 2254 3201 2011 Size: 104 139 947 4769 Fragments arranged by size: 4769 947 139 104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI