(Linear) (Six-base) MAPSORT of: KIEE1960.seq Check: 1501 from: 1 to: 1566 KIEE1960.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AccI GT'mk_AC Cuts at: 0 602 1091 1566 Size: 602 489 475 Fragments arranged by size: 602 489 475 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1417 1566 Size: 910 507 149 Fragments arranged by size: 910 507 149 AlwNI CAG_nnn'CTG Cuts at: 0 1383 1566 Size: 1383 183 ApaLI G'TGCA_C Cuts at: 0 740 1566 Size: 740 826 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 515 548 1566 Size: 515 33 1018 Fragments arranged by size: 1018 515 33 BanI G'GyrC_C Cuts at: 0 1263 1566 Size: 1263 303 BbsI GAAGACnn'nnnn_ Cuts at: 0 227 1427 1566 Size: 227 1200 139 Fragments arranged by size: 1200 227 139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1225 1259 1566 Size: 1225 34 307 Fragments arranged by size: 1225 307 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1350 1566 Size: 1350 216 BlpI GC'TnA_GC Cuts at: 0 168 1566 Size: 168 1398 Bme1580I G_kGCm'C Cuts at: 0 744 1159 1547 1566 Size: 744 415 388 19 Fragments arranged by size: 744 415 388 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 529 741 1566 Size: 175 354 212 825 Fragments arranged by size: 825 354 212 175 Bpu10I CC'TnA_GC Cuts at: 0 1256 1566 Size: 1256 310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 1566 Size: 100 1466 BsaI GGTCTCn'nnnn_ Cuts at: 0 759 1566 Size: 759 807 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1117 1147 1566 Size: 1117 30 419 Fragments arranged by size: 1117 419 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 235 1566 Size: 235 1331 BseYI C'CCAG_C Cuts at: 0 1502 1566 Size: 1502 64 BsiHKAI G_wGCw'C Cuts at: 0 744 1566 Size: 744 822 Bsp1286I G_dGCh'C Cuts at: 0 744 1159 1547 1566 Size: 744 415 388 19 Fragments arranged by size: 744 415 388 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 359 700 1566 Size: 359 341 866 Fragments arranged by size: 866 359 341 BsrGI T'GTAC_A Cuts at: 0 824 1566 Size: 824 742 BstXI CCAn_nnnn'nTGG Cuts at: 0 949 1566 Size: 949 617 BstYI r'GATC_y Cuts at: 0 204 923 1566 Size: 204 719 643 Fragments arranged by size: 719 643 204 BtgI C'CryG_G Cuts at: 0 1071 1566 Size: 1071 495 BtsI GCAGTG_nn' Cuts at: 0 1159 1439 1566 Size: 1159 280 127 Fragments arranged by size: 1159 280 127 EaeI y'GGCC_r Cuts at: 0 1319 1566 Size: 1319 247 EarI CTCTTCn'nnn_ Cuts at: 0 1101 1205 1461 1566 Size: 1101 104 256 105 Fragments arranged by size: 1101 256 105 104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 529 741 910 1417 1566 Size: 175 354 212 169 507 149 Fragments arranged by size: 507 354 212 175 169 149 EcoO109I rG'GnC_Cy Cuts at: 0 513 1107 1566 Size: 513 594 459 Fragments arranged by size: 594 513 459 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 439 684 1566 Size: 439 245 882 Fragments arranged by size: 882 439 245 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 915 947 1117 1119 1149 1151 1414 1446 Size: 915 32 170 2 30 2 263 32 Cuts at: 1446 1566 Size: 120 Fragments arranged by size: 915 263 170 120 32 32 30 2 2 HincII GTy'rAC Cuts at: 0 16 867 1449 1566 Size: 16 851 582 117 Fragments arranged by size: 851 582 117 16 HindIII A'AGCT_T Cuts at: 0 67 1566 Size: 67 1499 MscI TGG'CCA Cuts at: 0 1321 1566 Size: 1321 245 MslI CAynn'nnrTG Cuts at: 0 914 974 1566 Size: 914 60 592 Fragments arranged by size: 914 592 60 MspA1I CmG'CkG Cuts at: 0 820 1367 1566 Size: 820 547 199 Fragments arranged by size: 820 547 199 NcoI C'CATG_G Cuts at: 0 1071 1566 Size: 1071 495 NspI r_CATG'y Cuts at: 0 506 709 1354 1566 Size: 506 203 645 212 Fragments arranged by size: 645 506 212 203 PflMI CCAn_nnn'nTGG Cuts at: 0 105 1566 Size: 105 1461 PpuMI rG'GwC_Cy Cuts at: 0 513 1566 Size: 513 1053 PstI C_TGCA'G Cuts at: 0 278 1010 1154 1280 1566 Size: 278 732 144 126 286 Fragments arranged by size: 732 286 278 144 126 PvuII CAG'CTG Cuts at: 0 820 1367 1566 Size: 820 547 199 Fragments arranged by size: 820 547 199 SbfI CC_TGCA'GG Cuts at: 0 1280 1566 Size: 1280 286 SfcI C'TryA_G Cuts at: 0 274 599 1006 1150 1276 1566 Size: 274 325 407 144 126 290 Fragments arranged by size: 407 325 290 274 144 126 SmlI C'TyrA_G Cuts at: 0 8 115 1566 Size: 8 107 1451 Fragments arranged by size: 1451 107 8 SpeI A'CTAG_T Cuts at: 0 639 1530 1566 Size: 639 891 36 Fragments arranged by size: 891 639 36 SphI G_CATG'C Cuts at: 0 709 1566 Size: 709 857 StuI AGG'CCT Cuts at: 0 851 1198 1566 Size: 851 347 368 Fragments arranged by size: 851 368 347 StyI C'CwwG_G Cuts at: 0 1071 1203 1566 Size: 1071 132 363 Fragments arranged by size: 1071 363 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1281 1566 Size: 1281 285 TatI w'GTAC_w Cuts at: 0 824 1566 Size: 824 742 XbaI T'CTAG_A Cuts at: 0 465 1566 Size: 465 1101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 150 300 1078 1566 Size: 150 150 778 488 Fragments arranged by size: 778 488 150 150 Enzymes that do cut: AccI AcuI AlwNI ApaLI BaeI BanI BbsI BcgI BciVI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrGI BstXI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII MscI MslI MspA1I NcoI NspI PflMI PpuMI PstI PvuII SbfI SfcI SmlI SpeI SphI StuI StyI TaqII TatI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanII BbeI BbvCI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI TstI Tth111I XhoI XmaI XmnI ZraI